TREATMENT OF HEMOPHILIA WITH HUMAN FACTORIX PRODUCED IN MAMIMARY TISSUE OF TRANSGENIC MAMMALS [PDF]
, 2008 Recombinant Factor IX characterized by a high percentage of active protein can be obtained in the milk of transgenic animals that incorporate chimeric DNA molecules according to the present invention.
Drohan, William N. +4 more
core +1 more source
Isolation of β-globin related genes from a human cosmid library. [PDF]
, 1981 A human gene library was constructed using an improved cloning technique for cosmid vectors. Human placental DNA was partially digested with restriction endonuclease MboI; size-fractionated and ligated to BamHI-cut and phosphatase-treated cosmid vector ...
Boer, E. (Ernie) de +3 more
core +2 more sources
Light‐Triggered Protease‐Mediated Release of Actin‐Bound Cargo from Synthetic Cells
Advanced Biology, EarlyView.TEV Prtoease‐mediated Releasable Actin‐binding Protein (TRAP) is a protein‐based platform consisting of a cargo tightly bound to reconstituted actin networks in synthetic cells which can be proteolyticly released from the bound actin, followed by its secretion through membrane translocation mediated by a cell‐penetrating peptide.
Mousumi Akter +3 more
wiley +1 more source
Cloning of cDNA Encoding GRA1 Protein of Tachyzoite Toxoplasma Gondii Local Isolate
Indonesian Journal of Biotechnology, 2015 Gene encoding GRA1 protein is potent DNA-vaccine candidate against toxoplasmosis. The aim of the researchwas to clone the gene encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate by DNA recombinanttechnology.
Erma Sulistyaningsih +3 more
doaj
Virology Journal, 2011 Background Hepatitis B vaccine adjuvant, alum, is generally used for vaccination although it does not stimulate Th1 immunity and 10% of the population has low or no antibody response.
Wang Xingtai +4 more
doaj +1 more source
Commentary: A tribute to Dr. Paul Berg (1926-2023) American biochemist, Nobel Laureate and discoverer of recombinant DNA technology, vaccine and genetic engineering. [PDF]
Front Cell Dev Biol, 2023 Lukiw WJ.
europepmc +1 more source
Nonlocal Structural Effects of Water on DNA Homology Recognition [PDF]
The mechanism behind mutual recognition of homologous DNA sequences prior to genetic recombination is one of the remaining puzzles in molecular biology. Leading models of homology recognition, based on classical electrostatics, neglect the short-range nonlocal screening effects arising from structured water around DNA, and hence may only provide ...
arxiv +1 more source
Comparison of Plasmid yield between L.B. and using Molasses as a medium [PDF]
, 2009 During the initial studies I have found that the molasses may be a good alternative to costly L.B media where one can manipulate the C/N ratio as per requirement of cell.
Nitin Sharma, Purshottum Kaushik
core +2 more sources
Dissection of Mycobacterium tuberculosis antigens using recombinant DNA.
Proceedings of the National Academy of Sciences of the United States of America, 1985 A recombinant DNA strategy has been used systematically to survey the Mycobacterium tuberculosis genome for sequences that encode specific antigens detected by monoclonal antibodies. M. tuberculosis genomic DNA fragments with randomly generated endpoints
R. Young +5 more
semanticscholar +1 more source
Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
Advanced Biology, EarlyView.Bifunctional microgels carrying a linear DNA template and Ni2+‐activated NTA moieties are used as platform for cell‐free protein synthesis and in situ protein immobilization. By varying the concentration of NTA moieties in the microgels, the amount of GFP‐His immobilized inside the microgel and released to the microgel environment can be regulated ...
Anika Kaufmann +2 more
wiley +1 more source