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Parenteral viral hepatitis among students in Da Nang, Vietnam, and insights into new hepatitis B virus genotype B classification. [PDF]

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Kalinina OV   +7 more
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RECOMBINANT DNA TECHNOLOGY

The Lancet, 1981
Publisher Summary Plasmids are widely used as vectors and methods of their isolation can vary depending upon the host organism. On the basis of phenotypic effect and organization in the chromosomes, genes can be subdivided into various groups, such as simple genes, complex genes, operons, regulons, and multiple regulons.
openaire   +6 more sources

Introduction to recombinant DNA

Journal of Inherited Metabolic Disease, 1986
AbstractThis paper describes the current state of knowledge of methods for analysing gene structure and localization. Illustrations are given of the preparation of complementary DNA libraries and their screening by positive‐negative selection, the use of synthetic oligodeoxynucleotides and the use of antibodies. Analysis of the EGF precursor is used as
openaire   +3 more sources

Scarless DNA Recombineering

Cold Spring Harbor Protocols, 2023
The method described here allows editing of the bacterial genome without leaving any secondary changes (scars) behind. This method uses a tripartite selectable and counterselectable cassette comprising an antibiotic-resistance gene (catorkan) and thetetRrepressor gene linked to a Ptetpromoter-ccdBtoxin gene fusion.
Nara Figueroa-Bossi   +2 more
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Recombinant DNA

2003
Publisher Summary Recombinant DNA is the method of joining two or more DNA molecules to create a hybrid. The technology is made possible by two types of enzymes, restriction endonucleases and ligase. A restriction endonuclease recognizes a specific sequence of DNA and cuts within, or close to, that sequence.
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The Recombinant DNA Debate

1978
The debate over recombinant DNA research is a unique event, perhaps a turning point, in the history of science. For the first time in modern history there has been widespread public discussion about whether and how a promising though potentially dangerous line of research shall be pursued.
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Recombination in yeast and the recombinant DNA technology

Genome, 1989
The development of methods to isolate eukaryotic genes, alter these genes in vitro and reintroduce them into the cell has had a major impact on the study of recombination in the yeast Saccharomyces cerevisiae. In this paper we discuss how recombinant DNA techniques have been employed in the study of recombination in yeast and the results that have ...
Martin Kupiec   +9 more
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The recombinant DNA technology

Journal of Veterinary Pharmacology and Therapeutics, 1983
The recombinant DNA technology or DNA cloning permits the isolation, amplification, and precise manipulation of specific DNA fragments. This is generally accomplished by linking or recombining the desired DNA fragment with a DNA molecule, termed the vector, which is capable of directing the replication of itself in a suitable host cell and any DNA ...
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Recombinant DNA and the pediatrician

The Journal of Pediatrics, 1981
It is our opinion that the basic science clearly explained and illustrated in this article is surely worth understanding, in view of the several advances already established, the prospects which are imminent, and probably many other evolutions not yet anticipated.
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