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Cold Spring Harbor Protocols, 2023
The method described here allows editing of the bacterial genome without leaving any secondary changes (scars) behind. This method uses a tripartite selectable and counterselectable cassette comprising an antibiotic-resistance gene (catorkan) and thetetRrepressor gene linked to a Ptetpromoter-ccdBtoxin gene fusion.
Nara Figueroa-Bossi +2 more
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The method described here allows editing of the bacterial genome without leaving any secondary changes (scars) behind. This method uses a tripartite selectable and counterselectable cassette comprising an antibiotic-resistance gene (catorkan) and thetetRrepressor gene linked to a Ptetpromoter-ccdBtoxin gene fusion.
Nara Figueroa-Bossi +2 more
openaire +2 more sources
DNA Recombineering Applications
Cold Spring Harbor Protocols, 2023The ability to manipulate the bacterial genome is an obligatory premise for the study of gene function and regulation in bacterial cells. The λ red recombineering technique allows modification of chromosomal sequences with base-pair precision without the need of intermediate molecular cloning steps.
Nara Figueroa-Bossi +2 more
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Hiding Messages Based on DNA Sequence and Recombinant DNA Technique
IEEE transactions on nanotechnology, 2019Data hiding unnoticeably conceals message to prevent such message from being leaked to the existing media so as to ensure the confidentiality of the relevant information, which plays an important role in DNA cryptography. A data hiding model based on DNA
Yanfeng Wang +3 more
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The Lancet, 1981
Publisher Summary Plasmids are widely used as vectors and methods of their isolation can vary depending upon the host organism. On the basis of phenotypic effect and organization in the chromosomes, genes can be subdivided into various groups, such as simple genes, complex genes, operons, regulons, and multiple regulons.
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Publisher Summary Plasmids are widely used as vectors and methods of their isolation can vary depending upon the host organism. On the basis of phenotypic effect and organization in the chromosomes, genes can be subdivided into various groups, such as simple genes, complex genes, operons, regulons, and multiple regulons.
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Introduction to recombinant DNA
Journal of Inherited Metabolic Disease, 1986AbstractThis paper describes the current state of knowledge of methods for analysing gene structure and localization. Illustrations are given of the preparation of complementary DNA libraries and their screening by positive‐negative selection, the use of synthetic oligodeoxynucleotides and the use of antibodies. Analysis of the EGF precursor is used as
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A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD
Nature, 1998M. Enari +5 more
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