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Protein Engineering and Drug Discovery: Importance, Methodologies, Challenges, and Prospects. [PDF]
BiomoleculesMohammed A, Ibrahim NA, Basher NS.
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Studies on recombinant DNA techniques for cyanobacterium Spirulina platensis
, 2006 Yoshikazu Kawata
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A single donor cassette enables site-specific knock-in at either the αAmy3 or αAmy8 locus in rice cells via CRISPR/Cas9. [PDF]
Appl Microbiol BiotechnolSinaga DS +4 more
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Cold Spring Harbor Protocols, 2023
The method described here allows editing of the bacterial genome without leaving any secondary changes (scars) behind. This method uses a tripartite selectable and counterselectable cassette comprising an antibiotic-resistance gene (catorkan) and thetetRrepressor gene linked to a Ptetpromoter-ccdBtoxin gene fusion.
Nara Figueroa-Bossi +2 more
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The method described here allows editing of the bacterial genome without leaving any secondary changes (scars) behind. This method uses a tripartite selectable and counterselectable cassette comprising an antibiotic-resistance gene (catorkan) and thetetRrepressor gene linked to a Ptetpromoter-ccdBtoxin gene fusion.
Nara Figueroa-Bossi +2 more
openaire +2 more sources
DNA Recombineering Applications
Cold Spring Harbor Protocols, 2023The ability to manipulate the bacterial genome is an obligatory premise for the study of gene function and regulation in bacterial cells. The λ red recombineering technique allows modification of chromosomal sequences with base-pair precision without the need of intermediate molecular cloning steps.
Nara Figueroa-Bossi +2 more
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The Lancet, 1981
Publisher Summary Plasmids are widely used as vectors and methods of their isolation can vary depending upon the host organism. On the basis of phenotypic effect and organization in the chromosomes, genes can be subdivided into various groups, such as simple genes, complex genes, operons, regulons, and multiple regulons.
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Publisher Summary Plasmids are widely used as vectors and methods of their isolation can vary depending upon the host organism. On the basis of phenotypic effect and organization in the chromosomes, genes can be subdivided into various groups, such as simple genes, complex genes, operons, regulons, and multiple regulons.
openaire +4 more sources
Recombination in yeast and the recombinant DNA technology
Genome, 1989The development of methods to isolate eukaryotic genes, alter these genes in vitro and reintroduce them into the cell has had a major impact on the study of recombination in the yeast Saccharomyces cerevisiae. In this paper we discuss how recombinant DNA techniques have been employed in the study of recombination in yeast and the results that have ...
T D, Petes +9 more
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