Results 211 to 220 of about 577,747 (248)

Research Progress Fusion Tags for Recombinant Protein Production

Biotechnology and Applied Biochemistry
ABSTRACTRecombinant proteins are obtained using genetic engineering techniques and are widely used in various fields. Some recombinant proteins are difficult to express, purify, or are unstable or insoluble due to their structural characteristics. In order to address such issues, additional tags are fused at either the N‐ or C‐terminal end of the ...
Jing‐jia Yuan, Shao‐lei Geng, Tian‐yun Wang   +2 more
openaire   +2 more sources

Pathogenicity and immunogenicity of recombinant human retinal S-antigen fusion protein

Current Eye Research, 1992
A full-length cDNA clone to human S-antigen (HS-ag) was isolated from lambda gt 10 human retinal library and expressed as a fusion protein with glutathione S-transferase (GST) in E. Coli. Uveitogenicity and immunogenicity of recombinant GST-HS-ag fusion protein and native HS-ag were compared in EAU-susceptible Lewis rats.
R. Whiston, A. J. Roberts, Dudley C. Dumonde, J. P. Banga, E Kasp, Miles Stanford   +5 more
openaire   +3 more sources

Protein body-inducing fusions for recombinant protein production in plants.

2014
Abstract This chapter focuses on the use of plants as bioreactors for the production of recombinant proteins. The feasibility and promise of the use of protein body-inducing fusion tags in transient expression and in stable transgenic plants (i.e. tobacco) are also discussed.
Rima Menassa, S. Gutiérrez, K. L. Hefferon   +2 more
openaire   +2 more sources

Expression, Purification, and Immobilization of Recombinant Tamavidin 2 Fusion Proteins

2014
Tamavidin 2 is a fungal avidin-like protein that binds biotin with high affinity. Unlike avidin or streptavidin, tamavidin 2 in soluble form is produced at high levels in Escherichia coli. In this chapter, we describe a method for immobilization and purification of recombinant proteins with the use of tamavidin 2 as an affinity tag.
Masako Tsunashima, Yoshimitsu Takakura, Naomi Oka   +2 more
openaire   +3 more sources

A recombinant fusion protein clears IgA deposits

Nature Reviews Nephrology, 2022
openaire   +2 more sources

Recombinant Fusion Proteins Assembling Der p 1 and Der p 2 Allergens from Dermatophagoides pteronyssinus

International Archives of Allergy and Immunology, 2010
Laetitia Bussières, Véronique Bordas-Le Floch, I. Bulder, H. Chabre, E. Nony, Aurélie Lautrette, C. Berrouet, Y. Nguefeu, S. Horiot, V. Baron-Bodo, L. van Overtvelt, Anne Marie De Conti, A. Schlegel, Nicolas Maguet, N. Mouz, P. Lemoine, T. Batard, P. Moingeon   +17 more
semanticscholar   +1 more source

Covalent immobilization of recombinant fusion proteins with hAGT for single molecule force spectroscopy

European Biophysics Journal, 2005
Stefan K. Kufer, Hendrik Dietz, Christian H Albrecht, Kerstin G. Blank, A. Kardinal, M. Rief, H. Gaub   +6 more
semanticscholar   +1 more source

An evaluation of different enzymatic cleavage methods for recombinant fusion proteins, applied on des(1–3)insulin-like growth factor I

Journal of Protein Chemistry, 1992
G. Forsberg, B. Baastrup, Helena Rondahl, E. Holmgren, G. Pohl, M. Hartmanis, M. Lake   +6 more
semanticscholar   +1 more source

EFFECT OF VACCINATIONS WITH RECOMBINANT FUSION PROTEINS ON ANCYLOSTOMA CANINUM HABITAT SELECTION IN THE CANINE INTESTINE

Journal of Parasitology, 2002
P. Hotez, J. Ashcom, Z. Bin, J. Bethony, A. Williamson, J. Hawdon, Jianjun Feng, A. Dobardzic, Ivania M. Rizo, Janelle Bolden, Qun Jin, Wang Yan, R. Dobardzic, Sophia Chung-Debose, M. Crowell, Bennett J D Datu, Angela Delaney, Dilyan Dragonovski, Yang Jiang, Yueyuan Liu, K. Ghosh, A. Loukas, W. Brandt, P. Russell, B. Zook   +24 more
semanticscholar   +1 more source

Recombinant fusion proteins TAT‐Mu, Mu and Mu‐Mu mediate efficient non‐viral gene delivery

Journal of Gene Medicine, 2007
Rukkumani Rajagopalan, Jennifer Xavier, N. Rangaraj, N. Rao, V. Gopal   +4 more
semanticscholar   +1 more source