Results 261 to 270 of about 391,068 (308)

Architecting Multicompartmentalized, Giant Vesicles with Recombinant Fusion Proteins

Biomacromolecules
We present a straightforward strategy for constructing giant, multicompartmentalized vesicles using recombinant fusion proteins. Our method leverages the self-assembly of globule-zipper-elastin-like polypeptide fusion protein complexes in aqueous conditions, eliminating the need for organic solvents and chemical conjugation.
Jooyong Shin, Biswajit Saha, Hoyong Chung, Yeongseon Jang   +3 more
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Self-cleaving fusion tags for recombinant protein production

Biotechnology Letters, 2011
Fusion expression is a common practice for recombinant protein production. Some fusion tags confer solubility on the target protein whereas others provide affinity handles that facilitate purification. However, the tag usually needs to be removed from the final product, which involves using expensive proteases or hazardous chemicals and requires ...
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Antigenicity of a recombinant Ro (SS‐A) fusion protein

Arthritis & Rheumatism, 1990
AbstractThe antigenicity of the 60‐kd human Ro (SS‐A) synthesized in vitro from its complementary DNA as a β‐galactosidase fusion protein (β‐gal—Ro) was evaluated by Western blotting. In this analysis, almost all the anti‐Ro (SS‐A)‐positive sera that bound β‐gal‐Ro also bound affinity‐purified 60‐kd human Ro (SS‐A) (P > 0.005).
J A, James, W D, Dickey, A, Fujisaku, C A, O'Brien, S L, Deutscher, J D, Keene, J B, Harley   +6 more
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Recombinant fusion protein of albumin-retinol binding protein inactivates stellate cells

Biochemical and Biophysical Research Communications, 2012
Quiescent pancreatic- (PSCs) and hepatic- (HSCs) stellate cells store vitamin A (retinol) in lipid droplets via retinol binding protein (RBP) receptor and, when activated by profibrogenic stimuli, they transform into myofibroblast-like cells which play a key role in the fibrogenesis.
Soyoung, Choi, Sangeun, Park, Suhyun, Kim, Chaeseung, Lim, Jungho, Kim, Dae Ryong, Cha, Junseo, Oh   +6 more
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Recombinant peptide fusion proteins enable palladium nanoparticle growth

Materials Letters, 2019
Abstract The soluble fraction of bacterial lysates containing repeat units of a metal binding domain (Pd4) fused to the carrier protein Green Fluorescent Protein (GFPuv) was used to direct palladium nanoparticle synthesis on the order of 2 nm. Characterization confirmed the synthesis of highly ordered materials, as evident by microscopy and elemental
Mosleh, Imann, Benamara, Mourad, Greenlee, Lauren, Beyzavi, M. Hassan, Beitle, Robert   +4 more
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Different Approaches to Stabilize a Recombinant Fusion Protein

Nature Biotechnology, 1989
We have used a fusion protein between staphylococcal protein A and E. coli β–galactosidase as a model system to investigate different approaches to stabilize recombinant gene products. First, growth conditions were adapted to preferentially produce insoluble inclusion bodies.
Halldis Hellebust, Maria Murby, Lars Abrahmsén, Mathias Uhlén, Sven-Olof Enfors   +4 more
openaire   +1 more source

Research Progress Fusion Tags for Recombinant Protein Production

Biotechnology and Applied Biochemistry
ABSTRACTRecombinant proteins are obtained using genetic engineering techniques and are widely used in various fields. Some recombinant proteins are difficult to express, purify, or are unstable or insoluble due to their structural characteristics. In order to address such issues, additional tags are fused at either the N‐ or C‐terminal end of the ...
Jing‐jia Yuan, Shao‐lei Geng, Tian‐yun Wang   +2 more
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Recombinant peptide fusion construction for protein‐templated catalytic palladium nanoparticles

Biotechnology Progress, 2020
Abstract Although peptide‐enabled synthesis of nanostructures has garnered considerable interest for use in catalytic applications, it has so far been achieved mostly via Fmoc based solid phase peptide synthesis.
Rita Tejada‐Vaprio, Imann Mosleh, Rudra Palash Mukherjee, Hazim Aljewari, McKinzie Fruchtl, Ahmed Elmasheiti, Nicholas Bedford, Lauren Greenlee, M. Hassan Beyzavi, Robert Beitle   +9 more
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Staphylokinase as a Plasminogen Activator Component in Recombinant Fusion Proteins

Applied and Environmental Microbiology, 1999
ABSTRACT The plasminogen activator staphylokinase (SAK) is a promising thrombolytic agent for treatment of myocardial infarction. It can specifically stimulate the thrombolysis of both erythrocyte-rich and platelet-rich clots.
S J, Szarka, E G, Sihota, H R, Habibi, S, Wong   +3 more
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OPTIMIZING PROTEIN PURIFICATION FOR RNA-BINDING RECOMBINANT FUSION PROTEINS

2021
There has been great interest in delivering short interfering RNA (siRNA) and microRNA (miRNA) for therapeutic applications. However, the delivery of small RNAs remains challenging due to its inefficient cellular uptake and instability under physiological conditions.
openaire   +1 more source