Results 251 to 260 of about 262,160 (309)

Transposase‐Assisted Donor Tethering Boosts Large‐Fragment HDR in Plants

Advanced Science, EarlyView.
A transposase‐assisted donor tethering strategy is developed to enhance homology‐directed repair in plants. By recruiting donor DNA to double‐strand breaks and synergizing with repair pathway reprogramming and transcription‐coupled donor design, this system markedly improves large‐fragment targeted insertion efficiency, providing a robust platform for ...
Sha Wei, Kai Zhang, Shuke Deng, Jinjie Chen, Xiaomei Huang, Jingfei Guo, Yuqing Wu, Yingjie Guo, Zhen Liang   +8 more
wiley   +1 more source

Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6 in male mice. [PDF]

Bone Res
Pata M, Pham DNT, Lacombe J, Reddy BA, Kim YW, Ahmed AGA, Murshed M, Ferron M.   +7 more
europepmc   +1 more source

The landscape of antibody production systems: recombinant expression for research, diagnostics and therapy. [PDF]

Front Bioeng Biotechnol
Wenzel EV, Al-Halabi-Frenzel L, Demiral S, Frenzel A, Gnann DC, Keitel MC, Lehmler N, Mukherjee T, Rauls J, Schirrmann T, Stańczyk PG, Steinke S, Terbrack J, Westerhoff S, Dübel S, Schubert M, Hust M, Bertoglio F.   +17 more
europepmc   +1 more source

The p40 fusion domain as a scaffold for producing functional inclusion bodies. [PDF]

Appl Microbiol Biotechnol
Vijayakumar S, Khetan R, Sunna A.
europepmc   +1 more source

Viral vector-based imaging uncovers infection-induced re-localization of pepino mosaic virus proteins. [PDF]

J Virol
Zhang J, Li Z, He H, Zhou X, Li F.
europepmc   +1 more source

Nonunion After Ankle Arthrodesis: A Contemporary Review. [PDF]

Foot Ankle Int
Rizk RC, Liebmann K, Rivera CA, Rodriguez-Materon S.   +3 more
europepmc   +1 more source

Deep Learning-Enabled Engineering of a Hyper-Stable and Soluble MPB70-83 Antigen for Sensitive Bovine Tuberculosis Surveillance. [PDF]

Microb Biotechnol
Wang WH, Ge JZ, Xie YY, Song GD, Liu Y, Song YJ, Li RG, Li RS, Wang ZQ, Liu XM, Guo SS, Li J, Chen S, Li N, Zheng F, Chu Y.   +15 more
europepmc   +1 more source

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Enhancement of the solubility of recombinant proteins by fusion with a short-disordered peptide

Journal of Microbiology, 2022
In protein biotechnology, large soluble fusion partners are widely utilized for increased yield and solubility of recombinant proteins. However, the production of additional large fusion partners poses an additional burden to the host, leading to a decreased protein yield. In this study, we identified two highly disordered short peptides that were able
Dokyun Na, Na Dokyun
exaly   +3 more sources

Production of recombinant proteins in Escherichia coli tagged with the fusion protein CusF3H+

Protein Expression and Purification, 2017
Recombinant protein expression in the bacterium Escherichia coli still is the number one choice for large-scale protein production. Nevertheless, many complications can arise using this microorganism, such as low yields, the formation of inclusion bodies, and the requirement for difficult purification steps.
TERESA Vargas-Cortez, JOSÉ Ruben Morones-Ramírez, Isaias Balderas-Rentería   +2 more
exaly   +3 more sources

Recombinant avidin and avidin–fusion proteins

Biomolecular Engineering, 1999
Both chicken egg-white avidin and its bacterial relative streptavidin are well known for their extraordinary high affinity with biotin (Kd approximately 10(-15) M). They are widely used as tools in a number of affinity-based separations, in diagnostic assays and in a variety of other applications. These methods have collectively become known as (strept)
K J, Airenne, V S, Marjomäki, M S, Kulomaa   +2 more
openaire   +2 more sources