Results 271 to 280 of about 391,068 (308)
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Experimental Spinal Fusion With Recombinant Human Bone Morphogenetic Protein-2
Spine, 1995Lumbar intertransverse process arthrodesis using recombinant human bone morphogenetic protein-2 was performed in a previously established rabbit model for posterolateral spinal fusion and compared with fusions achieved using autogenous bone graft.To qualitatively compare different recombinant human bone morphogenetic protein-2 dosages and carriers and ...
J H, Schimandle, S D, Boden, W C, Hutton
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Recombinant rubella E1 fusion proteins for antibody screening and diagnosis
Clinical and Diagnostic Virology, 1994Until rubella is eradicated there will be a continuing need for rubella antibody surveillance. Antigen production using recombinant DNA technology may be a viable alternative to traditional techniques of producing antigens for enzyme immunoassays (EIAs).To investigate the potential of bacterial fusion proteins containing rubella E1 protein sequences ...
J, Newcombe +5 more
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The Use of Recombinant Fusion Proteases in the Affinity Purification of Recombinant Proteins
Molecular Biotechnology, 1999In the affinity purification of recombinant fusion proteins, the rate-limiting step is usually the efficient proteolytic cleavage and removal of the affinity tail and the protease from the purified recombinant protein. We have developed a rapid, convenient, and efficient method of affinity purification that can overcome this limitation.
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Production of Recombinant Oxytocin Through Sulfitolysis of Inteincontaining Fusion Protein
Protein & Peptide Letters, 2012An artificial gene consisting of seven copies of an oxytocinoyl-lysine encoding sequence arranged in a tandem was synthesized and inserted downstream of the SspDnaB intein gene in a pTWIN1 plasmid. The corresponding fusion protein Dnab-7oxy contained 16 cysteine residues and formed inclusion bodies when expressed in E. coli.
Roman S, Esipov +3 more
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Fusion tails for the recovery and purification of recombinant proteins
Protein Expression and Purification, 1991Several fusion tail systems have been developed to promote efficient recovery and purification of recombinant proteins from crude cell extracts or culture media. In these systems, a target protein is genetically engineered to contain a C- or N-terminal polypeptide tail, which provides the biochemical basis for specificity in recovery and purification ...
C F, Ford, I, Suominen, C E, Glatz
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Tag Removal by Site-Specific Cleavage of Recombinant Fusion Proteins
2010Where an affinity tag has served its purpose it may become desirable to remove it from the protein of interest. This chapter describes the removal of such fusion partners from the intended protein product by cleavage with site-specific endoproteases. Methods to achieve proteolytic cleavage of the fusion proteins are provided, along with techniques for ...
Adam, Charlton, Michael, Zachariou
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Expression, Purification, and Immobilization of Recombinant Tamavidin 2 Fusion Proteins
2014Tamavidin 2 is a fungal avidin-like protein that binds biotin with high affinity. Unlike avidin or streptavidin, tamavidin 2 in soluble form is produced at high levels in Escherichia coli. In this chapter, we describe a method for immobilization and purification of recombinant proteins with the use of tamavidin 2 as an affinity tag.
Yoshimitsu, Takakura +2 more
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A recombinant fusion protein clears IgA deposits
Nature Reviews Nephrology, 2022openaire +2 more sources
Recombinant TLR-MD-2 fusion proteins
2006The present invention provides recombinant TLR-MD-2 fusion proteins capable of binding to lipopolysaccharide (LPS) containing a TLR-moiety comprising the extracellular domain of a Toll-like receptor (TLR) or a functional derivative thereof, fused with a MD-2-moiety comprising MD-2 or a functional derivative thereof.
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Integrative oncology: Addressing the global challenges of cancer prevention and treatment
Ca-A Cancer Journal for Clinicians, 2022Jun J Mao,, Msce +2 more
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