Results 281 to 290 of about 262,160 (309)

Affinity Fusion Strategies for Detection, Purification, and Immobilization of Recombinant Proteins

Protein Expression and Purification, 1997
Genetic engineering enables the construction of gene man Genome Project (5), efficient and robust production and purification strategies are necessary (6). For fusions resulting in fusion proteins having the combined properties of the original gene products.
J, Nilsson, S, Ståhl, J, Lundeberg, M, Uhlén, P A, Nygren   +4 more
openaire   +2 more sources

Recombinant rubella E1 fusion proteins for antibody screening and diagnosis

Clinical and Diagnostic Virology, 1994
Until rubella is eradicated there will be a continuing need for rubella antibody surveillance. Antigen production using recombinant DNA technology may be a viable alternative to traditional techniques of producing antigens for enzyme immunoassays (EIAs).To investigate the potential of bacterial fusion proteins containing rubella E1 protein sequences ...
J, Newcombe, W, Starkey, S, Al-Mumin, A I, Knight, J M, Best, P G, Sanders   +5 more
openaire   +2 more sources

Recombinant Human Hb‐SOD Fusion Proteins

2013
Hemoglobin (Hb) can produce reactive oxygen species, including superoxide anions, which are intrinsically toxic. Superoxide dismutase (SOD) is present in red blood cells (RBCs) and provides important protection against such oxidative stress. Upon hemolysis, Hb becomes released from the RBCs and the normal protection systems involving SOD and catalase ...
Marie Grey, Khuanpiroon Ratanasopa, Leif Bülow   +2 more
openaire   +1 more source

Population pharmacokinetics of recombinant factor VIII Fc fusion protein

Clinical Pharmacology in Drug Development, 2014
AbstractPopulation pharmacokinetics (PK) of FVIII activity‐time profiles following recombinant factor VIII Fc fusion protein (rFVIIIFc) and recombinant factor VIII (rFVIII) dosing were evaluated in previously treated patients with severe hemophilia A (from two clinical trials).
Ivan, Nestorov, Srividya, Neelakantan, Thomas M, Ludden, Shuanglian, Li, Haiyan, Jiang, Mark, Rogge   +5 more
openaire   +2 more sources

Expression, Purification, and Immobilization of Recombinant Tamavidin 2 Fusion Proteins

2014
Tamavidin 2 is a fungal avidin-like protein that binds biotin with high affinity. Unlike avidin or streptavidin, tamavidin 2 in soluble form is produced at high levels in Escherichia coli. In this chapter, we describe a method for immobilization and purification of recombinant proteins with the use of tamavidin 2 as an affinity tag.
Yoshimitsu, Takakura, Naomi, Oka, Masako, Tsunashima   +2 more
openaire   +2 more sources

Tag Removal by Site-Specific Cleavage of Recombinant Fusion Proteins

2010
Where an affinity tag has served its purpose it may become desirable to remove it from the protein of interest. This chapter describes the removal of such fusion partners from the intended protein product by cleavage with site-specific endoproteases. Methods to achieve proteolytic cleavage of the fusion proteins are provided, along with techniques for ...
Adam, Charlton, Michael, Zachariou
openaire   +2 more sources

A recombinant fusion protein clears IgA deposits

Nature Reviews Nephrology, 2022
openaire   +2 more sources

Magnetic Affinity Separation of Recombinant Fusion Proteins

Isolation and separation of proteins is an important procedure in all areas of biosciences and biotechnology. Currently recombinant fusion proteins are produced routinely; in order to simplify their isolation from complex samples, magnetic separation techniques can be efficiently used. This short review paper shows examples of the most important fusion
SAFARİK, Ivo, SAFARİKOVA, Mirka
openaire   +1 more source

Applications of novel affinity cassette methods: use of peptide fusion handles for the purification of recombinant proteins

Journal of Molecular Recognition, 2001
Milton T W Hearn
exaly  

Recombinant EphB4-HSA Fusion Protein

2020
openaire   +1 more source