Results 161 to 170 of about 1,066,221 (297)

Characterization of endogenous promoters of GapC1 and GS for recombinant protein expression in Phaeodactylum tricornutum. [PDF]

Microbiologyopen, 2021
Erdene-Ochir E, Shin BK, Huda MN, Lee EH, Song DG, Jung C, Pan CH.   +6 more
europepmc   +1 more source

Soft Neural Interfaces for Circuit‐Level Analysis of Magnetogenetic Deep Brain Stimulation in Parkinson's Disease Models

Advanced Healthcare Materials, EarlyView.
ABSTRACT Magnetogenetic deep brain stimulation (MG‐DBS) represents a wireless neuromodulation that has demonstrated long‐lasting behavioral benefits in Parkinson's disease models. However, the circuit‐level mechanisms underlying these therapeutic effects have remained uncharacterized due to limitations of conventional neural interfaces.
Jakyoung Lee, Yeongdo Lee, Enji Kim, Hunkyu Seo, Myoungjae Oh, Hanho Joo, Somin Lee, Jae‐Hyun Lee, Hyun Ho Jung, Minsuk Kwak, Jang‐Ung Park   +10 more
wiley   +1 more source

Nanotherapies for Atherosclerosis: Targeting, Catalysis, and Energy Transduction

Advanced Healthcare Materials, EarlyView.
Atherosclerosis management is hindered by poor drug targeting and plaque heterogeneity. Nanotechnology overcomes these barriers via three core strategies: (1) target‐engineered nanocarriers that achieve lesion‐specific precision via ligand modification, biomimetic camouflage, stimuli‐responsive release, and self‐propelling nanomotors; (2) catalytic ...
Yuqi Yang, Jingguo Qu, Tonghuan Xiao, Xinyang Han, Michael Tin‐Cheung Ying   +4 more
wiley   +1 more source

A review of alternative promoters for optimal recombinant protein expression in baculovirus-infected insect cells. [PDF]

Protein Expr Purif, 2021
Grose C, Putman Z, Esposito D.
europepmc   +1 more source

Plant‐Produced Viral Nanoparticles Decorated with Nanobodies Against HER2 Improve Retention and Recruitment of Immune Cells in Solid Tumors

Advanced Healthcare Materials, EarlyView.
Potato virus X is a filamentous RNA plant virus that can be engineered into a molecular tool for cancer therapy. We produced genetically‐encoded virus‐derived nanoparticles decorated with nanobodies targeting cancer cell receptors, epidermal growth factor receptor (EGFR) and human epidermal growth factor 2 (HER2).
Enrique Lozano‐Sanchez, Miguel A. Moreno‐Gonzalez, Fernando Merwaiss, Nicole F. Steinmetz, José‐Antonio Daròs   +4 more
wiley   +1 more source

Choice of selectable marker affects recombinant protein expression in cells and exosomes. [PDF]

J Biol Chem, 2021
Guo C, Fordjour FK, Tsai SJ, Morrell JC, Gould SJ.   +4 more
europepmc   +1 more source

A Physiological Microfluidic Blood–Brain‐Barrier Model for In Vitro Study of Nanoparticle Trafficking and Accumulation

Advanced Healthcare Materials, EarlyView.
A human microfluidic blood‐brain barrier (mBBB) model enables spatially resolved comparison of nanoparticle trafficking. Extracellular vesicles (EVs), liposomes, and nanoplastics exhibit distinct transport and disruption behaviors, revealing that membrane composition and uptake pathways govern BBB interaction.
Bryan B. Nguyen, Neona M. Lowe, Sophia Kellogg, Kuan‐Wei Huang, Hannah O'Toole, Elizabeth J. Hale, Venktesh S. Shirure, Bhupinder S. Shergill, Steven C. George, Randy P. Carney   +9 more
wiley   +1 more source

EXPRESSION AND PURIFICATION OF RECOMBINANT PHENYLALANINE AMMONIA-LYASE FROM PETROSELINUM CRISPUM

Studia Universitatis Babes-Bolyai Chemia, 2016
In the present study the molecular cloning, expression and purification of recombinant PcPAL, with a cleavable N-terminal His-tag is described. The PcPAL gene was cloned into pET-19b vector and transformed to different E.coli host cells.
Norbert Artur DIMA, Alina FILIP, László Csaba BENCZE, Márk OLÁH, Péter SÁTORHELYI, Beáta G. VÉRTESSY, László POPPE, Csaba PAIZS   +7 more
doaj  

3D Soft Hydrogels Induce Human Mesenchymal Stem Cells “Deep” Quiescence

Advanced Healthcare Materials, EarlyView.
Three‐dimensional soft hydrogels mimicking the bone marrow niche induce deep quiescence in human mesenchymal stem cells. Unlike 2D culture, 3D matrices halt proliferation, regulate cell‐cycle and quiescence markers, and downregulate mTORC1 signaling, preserving stem cell phenotype and therapeutic potential ex vivo.
David Boaventura Gomes, Timo Rademakers, Ana Filipa Henriques Lourenço, Andrea Calore, Clarissa Tomasina, Denis van Beurden, Jinjie Venema, Bryan Chömpff, Hong Liu, Nicole Bouvy, Sandra Camarero‐Espinosa, Lorenzo Moroni   +11 more
wiley   +1 more source

Insights into the Performance of CusF as a Solubility Tag for Recombinant Protein Expression. [PDF]

Int J Mol Sci
Oscorbin IP, Smertina MA, Kunova MS, Filipenko ML.   +3 more
europepmc   +1 more source