Results 1 to 10 of about 1,309,435 (302)

Leveraging ANXA1 to enhance recombinant protein yields in CHO cells: A UPR-Mediated bioprocessing approach [PDF]

Synthetic and Systems Biotechnology
Chinese hamster ovary (CHO) cells undergo endoplasmic reticulum stress (ERS) during intensive recombinant protein production, triggering the unfolded protein response (UPR) to balance cell survival and protein output.
Qi Zhao, Hui-Jie Zhang, Ming-Ming Han, Jumai Abiti, Yan-Ju Dong, Jia-Ning Wang, Jiang-Tao Lu, Wen Wang, Xi Zhang, Shao-Lei Geng, Le-Le Qiu, Xiao-Yin Wang, Zi-Chun Hua, Tian-Yun Wang, Yan-Long Jia   +14 more
doaj   +2 more sources

SETDB1 knockdown boosts recombinant protein in CHO cells via epigenetic transcriptional silencing [PDF]

AMB Express
Chinese hamster ovary (CHO) cells serve as the predominant mammalian expression system for recombinant protein production. However, clonal heterogeneity and instability in recombinant protein expression remain significant challenges.
Jiang-Tao Lu, Miao Zhang, Yan-Ping Gao, Lu-Lu Yang, Zhao-Ming Cui, Ming-Ming Han, Xi Zhang, Hong-Yan Xu, Xiao-Yin Wang, Le-Le Qiu, Zi-Chun Hua, Tian-Yun Wang, Yan-Long Jia   +12 more
doaj   +2 more sources

Epigenetic regulation of histone methyltransferase SUV39H1 on the expression of recombinant protein in CHO cells [PDF]

Applied Microbiology and Biotechnology
Abstract Histone methylation–mediated epigenetic modification significantly influences gene transcription and expression regulation. This study examined the effects of histone 3 lysine 9 trimethylation (H3K9me3) methyltransferase SUV39H1 and its specific
Lu-Lu Yang, Miao Zhang, Jia-Liang Guo, Ming-Ming Han, Le-Le Qiu, Li-Jie Gao, Liu-Su Cui, Xiao-Yin Wang, Tian-Yun Wang, Yan-Long Jia   +9 more
doaj   +2 more sources

Chemical Chaperones Improve Protein Secretion and Rescue Mutant Factor VIII in Mice with Hemophilia A. [PDF]

, 2012
nefficient intracellular protein trafficking is a critical issue in the pathogenesis of a variety of diseases and in recombinant protein production.
Abriss, Daniela, Milanov, Peter, Pepperkok, Rainer, Quade-Lyssy, Patricia, Roth, Stefanie D., Schüttrumpf, Jörg, Seifried, Erhard, Simpson, Jeremy C., Tonn, Torsten, Ungerer, Christopher   +9 more
core   +23 more sources

Isolation and characterization of Brochothrix phage ADU4

Virus Research, 2022
B. thermosphacta is a psychrotrophic bacterium that often forms the predominant part of the spoilage microflora of aerobically and anaerobically stored meats.
Abdulkerim Karaynir, Hanife Salih, Bülent Bozdoğan, Özgür Güçlü, Dilek Keskin   +4 more
doaj   +1 more source

Highly Expressed Soluble Recombinant Anti-GFP VHHs in Escherichia coli via Optimized Signal Peptides, Strains, and Inducers

Frontiers in Molecular Biosciences, 2022
Antigen-binding variable domains of the H chain of heavy-chain antibodies (VHHs), also known as nanobodies (Nbs), are of great interest in imaging technique, disease prevention, diagnosis, and therapy.
Shuangying Chao, Shuangying Chao, Yuhang Liu, Yuhang Liu, Ning Ding, Ning Ding, Yue Lin, Yue Lin, Qian Wang, Qian Wang, Junwen Tan, Junwen Tan, Wei Li, Wei Li, Yang Zheng, Yang Zheng, Xuejun Hu, Xuejun Hu, Junming Li   +18 more
doaj   +1 more source

Triple-negative breast cancer: understanding Wnt signaling in drug resistance

Cancer Cell International, 2021
Triple-negative breast cancer (TNBC) is not as prevalent as hormone receptor or HER2-positive breast cancers and all receptor tests come back negative.
Parnaz Merikhian, Mohammad Reza Eisavand, Leila Farahmand   +2 more
doaj   +1 more source

Stabilizing and Anti-Repressor Elements Effectively Increases Transgene Expression in Transfected CHO Cells

Frontiers in Bioengineering and Biotechnology, 2022
Chinese hamster ovary (CHO) cells are currently the most widely used host cells for recombinant therapeutic protein (RTP) production. Currently, the RTP yields need to increase further to meet the market needs and reduce costs.
Qin Li, Qin Li, Rui-Fang Yan, Yong-Xiao Yang, Chun-liu Mi, Chun-liu Mi, Yan-long Jia, Tian-Yun Wang, Tian-Yun Wang   +8 more
doaj   +1 more source

Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells

Frontiers in Bioengineering and Biotechnology, 2022
In recent years, mammalian cells have become the primary host cells for the production of recombinant therapeutic proteins (RTPs). Despite that the expression of RTPs in mammalian cells can be improved by directly optimizing or engineering the expression
Mian Wei, Mian Wei, Chun-Liu Mi, Chang-Qin Jing, Tian-Yun Wang   +4 more
doaj   +1 more source

Dual blockade of CD47 and CD24 signaling using a novel bispecific antibody fusion protein enhances macrophage immunotherapy

Molecular Therapy: Oncolytics, 2023
CD47 and its receptor signal regulatory protein α (SIRPα) act as a dominant antiphagocytic, “don’t eat me” signal. Recent studies reveal CD24 as a novel target for cancer immunotherapy by macrophages in ovarian cancer and breast cancer.
Yun Yang, He Wu, Yan Yang, Yan Kang, Runjia He, Bei Zhou, Huaizu Guo, Jing Zhang, Jianqin Li, Chunpo Ge, Tianyun Wang   +10 more
doaj   +1 more source