Results 1 to 10 of about 7,683,339 (208)
Frontiers in Bioengineering and Biotechnology, 2022 Recombinant therapeutic proteins (RTPs) are important parts of biopharmaceuticals. Chinese hamster ovary cells (CHO) have become the main cell hosts for the production of most RTPs approved for marketing because of their high-density suspension growth ...
Zheng-Mei Li +7 more
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New tools for recombinant protein production in Escherichia coli: A 5‐year update
Protein Science, 2019 The production of proteins in sufficient amounts is key for their study or use as biotherapeutic agents. Escherichia coli is the host of choice for recombinant protein production given its fast growth, easy manipulation, and cost‐effectiveness.
Eduardo A Ceccarelli, Rosano German L
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Perfusion mammalian cell culture for recombinant protein manufacturing – A critical review
Biotechnology Advances, 2018 The manufacturing of recombinant protein is traditionally divided in two main steps: upstream (cell culture and synthesis of the target protein) and downstream (purification and formulation of the protein into a drug substance or drug product).
Massimo Morbidelli +2 more
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Protein Expression and PurificationE. coli plays a substantial role in recombinant protein production. Its importance increased with the discovery of recombinant DNA technology and the subsequent production of the first recombinant insulin in E. coli. E.
Özlem Kaplan, Ibrahim İncir
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Comparison of Yeasts as Hosts for Recombinant Protein Production
Microorganisms, 2018 Recombinant protein production emerged in the early 1980s with the development of genetic engineering tools, which represented a compelling alternative to protein extraction from natural sources.
Frederico Mendonça Bahia, Parachin N S
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Polyionic Tags as Enhancers of Protein Solubility in Recombinant Protein Expression
Microorganisms, 2018 Since the introduction of recombinant protein expression in the second half of the 1970s, the growth of the biopharmaceutical field has been rapid and protein therapeutics has come to the foreground.
Vasiliki Paraskevopoulou +1 more
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Leveraging ANXA1 to enhance recombinant protein yields in CHO cells: A UPR-Mediated bioprocessing approach [PDF]
Synthetic and Systems BiotechnologyChinese hamster ovary (CHO) cells undergo endoplasmic reticulum stress (ERS) during intensive recombinant protein production, triggering the unfolded protein response (UPR) to balance cell survival and protein output.
Qi Zhao +14 more
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Bioengineering, 2018 The production of high yields of soluble recombinant protein is one of the main objectives of protein biotechnology. Several factors, such as expression system, vector, host, media composition and induction conditions can influence recombinant protein ...
Barry Ryan +2 more
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Open Biology, 2016 The ease of genetic manipulation, low cost, rapid growth and number of previous studies have made Escherichia coli one of the most widely used microorganism species for producing recombinant proteins.
Baolei Jia, Che Ok Jeon
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Epigenetic regulation of histone methyltransferase SUV39H1 on the expression of recombinant protein in CHO cells [PDF]
Applied Microbiology and BiotechnologyAbstract Histone methylation–mediated epigenetic modification significantly influences gene transcription and expression regulation. This study examined the effects of histone 3 lysine 9 trimethylation (H3K9me3) methyltransferase SUV39H1 and its specific
Lu-Lu Yang +9 more
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