Results 11 to 20 of about 7,683,438 (307)

SETDB1 knockdown boosts recombinant protein in CHO cells via epigenetic transcriptional silencing [PDF]

AMB Express
Chinese hamster ovary (CHO) cells serve as the predominant mammalian expression system for recombinant protein production. However, clonal heterogeneity and instability in recombinant protein expression remain significant challenges.
Jiang-Tao Lu, Miao Zhang, Yan-Ping Gao, Lu-Lu Yang, Zhao-Ming Cui, Ming-Ming Han, Xi Zhang, Hong-Yan Xu, Xiao-Yin Wang, Le-Le Qiu, Zi-Chun Hua, Tian-Yun Wang, Yan-Long Jia   +12 more
doaj   +2 more sources

Isolation and characterization of Brochothrix phage ADU4

Virus Research, 2022
B. thermosphacta is a psychrotrophic bacterium that often forms the predominant part of the spoilage microflora of aerobically and anaerobically stored meats.
Abdulkerim Karaynir, Hanife Salih, Bülent Bozdoğan, Özgür Güçlü, Dilek Keskin   +4 more
doaj   +1 more source

Progress in fed-batch culture for recombinant protein production in CHO cells

Applied Microbiology and Biotechnology, 2023
Nearly 80% of the approved human therapeutic antibodies are produced by Chinese Hamster Ovary (CHO) cells. To achieve better cell growth and high-yield recombinant protein, fed-batch culture is typically used for recombinant protein production in CHO ...
Wen-Jing Xu, Yan Lin, Chun-liu Mi, Jing Pang, Tian-yun Wang   +4 more
semanticscholar   +1 more source

Improving recombinant protein production by yeast through genome-scale modeling using proteome constraints

Nature Communications, 2022
Eukaryotic cells are used as cell factories to produce and secrete multitudes of recombinant pharmaceutical proteins, including several of the current top-selling drugs.
Feiran Li, Yu Chen, Qi Qi, Yanyan Wang, Le Yuan, Mingtao Huang, I. E. Elsemman, A. Feizi, E. Kerkhoven, J. Nielsen   +9 more
semanticscholar   +1 more source

Impact of the Expression System on Recombinant Protein Production in Escherichia coli BL21

Frontiers in Microbiology, 2021
Recombinant protein production for medical, academic, or industrial applications is essential for our current life. Recombinant proteins are obtained mainly through microbial fermentation, with Escherichia coli being the host most used. In spite of that,
G. Lozano Terol, J. Gallego-Jara, R. A. Sola Martínez, Adrián Martínez Vivancos, M. Cánovas Díaz, T. de Diego Puente   +5 more
semanticscholar   +1 more source

Highly Expressed Soluble Recombinant Anti-GFP VHHs in Escherichia coli via Optimized Signal Peptides, Strains, and Inducers

Frontiers in Molecular Biosciences, 2022
Antigen-binding variable domains of the H chain of heavy-chain antibodies (VHHs), also known as nanobodies (Nbs), are of great interest in imaging technique, disease prevention, diagnosis, and therapy.
Shuangying Chao, Shuangying Chao, Yuhang Liu, Yuhang Liu, Ning Ding, Ning Ding, Yue Lin, Yue Lin, Qian Wang, Qian Wang, Junwen Tan, Junwen Tan, Wei Li, Wei Li, Yang Zheng, Yang Zheng, Xuejun Hu, Xuejun Hu, Junming Li   +18 more
doaj   +1 more source

Challenges Associated With the Formation of Recombinant Protein Inclusion Bodies in Escherichia coli and Strategies to Address Them for Industrial Applications

Frontiers in Bioengineering and Biotechnology, 2021
Recombinant proteins are becoming increasingly important for industrial applications, where Escherichia coli is the most widely used bacterial host for their production. However, the formation of inclusion bodies is a frequently encountered challenge for
Arshpreet Bhatwa, Weijun Wang, Y. Hassan, Nadine Abraham, Xiu-zhen Li, Ting Zhou   +5 more
semanticscholar   +1 more source

Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production

Metabolites, 2022
Komagataella phaffii (formerly known as Pichia pastoris) has become an increasingly important microorganism for recombinant protein production. This yeast species has gained high interest in an industrial setting for the production of a wide range of ...
C. Bustos, J. Quezada, Rhonda Veas, C. Altamirano, S. Braun-Galleani, P. Fickers, J. Berrios   +6 more
semanticscholar   +1 more source

Triple-negative breast cancer: understanding Wnt signaling in drug resistance

Cancer Cell International, 2021
Triple-negative breast cancer (TNBC) is not as prevalent as hormone receptor or HER2-positive breast cancers and all receptor tests come back negative.
Parnaz Merikhian, Mohammad Reza Eisavand, Leila Farahmand   +2 more
doaj   +1 more source

TISIGNER.com: web services for improving recombinant protein production

Nucleic Acids Res., 2021
Experiments that are planned using accurate prediction algorithms will mitigate failures in recombinant protein production. We have developed TISIGNER (https://tisigner.com) with the aim of addressing technical challenges to recombinant protein ...
B. Bhandari, C. Lim, P. Gardner
semanticscholar   +1 more source