Physiological aspects underpinning recombinant protein production in Escherichia coli [PDF]
, 2011 Many biopharmaceutical projects require the production of recombinant protein in a bacterial host. Conventional procedures used for recombinant protein production (RPP) involve the rapid synthesis of the target protein.
Alfasi, Sara Nuri
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The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism
Frontiers in Bioengineering and Biotechnology, 2022 Recombinant protein production by mammalian cells is the initial step in the manufacture of many therapeutic proteins. Chinese hamster ovary (CHO) cells are the most common host system to produce recombinant therapeutic proteins (RTPs).
Hui-Ning Liu +7 more
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Maltose-binding protein enhances secretion of recombinant human granzyme B accompanied by in vivo processing of a precursor MBP fusion protein [PDF]
, 2010 Background: The apoptosis-inducing serine protease granzyme B (GrB) is an important factor contributing to lysis of target cells by cytotoxic lymphocytes.
Wels, Winfried +14 more
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An outlook on coronavirus disease 2019 detection methods
Journal of Pharmaceutical Analysis, 2022 Diagnostic testing plays a fundamental role in the mitigation and containment of coronavirus disease 2019 (COVID-19), as it enables immediate quarantine of those who are infected and contagious and is essential for the epidemiological characterization of
Zahra Hassani Nejad +2 more
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Improvement of downstream processing of recombinant proteins by means of genetic engineering [PDF]
, 1993 Flaschel E, Friehs K. Improvement of downstream processing of recombinant proteins by means of genetic engineering. Biotechnology Advances. 1993;11(1):31-77.The rapid advancement of genetic engineering has allowed to produce an impressive number of ...
Flaschel, Erwin, Friehs, Karl
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Screening Strategies for High-Yield Chinese Hamster Ovary Cell Clones
Frontiers in Bioengineering and Biotechnology, 2022 Chinese hamster ovary (CHO) cells are by far the most commonly used mammalian expression system for recombinant expression of therapeutic proteins in the pharmaceutical industry.
Wenwen Yang +8 more
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Expression of recombinant multi-coloured fluorescent antibodies in
, 2011 Background Antibody-fluorophore conjugates are invaluable reagents used in contemporary molecular cell biology for imaging, cell sorting and tracking intracellular events.
Durvasula, Ravi V. +26 more
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Engineering a novel endopeptidase based on SARS 3CLpro
BioTechniques, 2009 A 3C-like protease (3CLpro) from the severe acute respiratory syndrome–coronavirus (SARS-CoV) is required for viral replication, cleaving the replicase polyproteins at 11 sites with the conserved Gln↓(Ser, Ala, Gly) sequences. In this study, we developed
Chih-Jung Kuo +3 more
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Recombinant human preproinsulin expression, purification and reaction with insulin autoantibodies in sera from patients with insulin-dependent diabetes mellitus [PDF]
, 1993 A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant fusion protein with a histidine-hexapeptide and glutathione-S-transferase at its N-terminus and the recombinant human preproinsulin at its C-terminus ...
Seißler, Jochen +4 more
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Multi-Compartment and Multi-Host Vector Suite for Recombinant Protein Expression and Purification
Frontiers in Microbiology, 2018 Recombinant protein expression has become an invaluable tool in basic and applied research. The accumulated knowledge in this field allowed the expression of thousands of protein targets in a soluble, pure, and homogeneous state, essential for ...
Claudia Ortega +9 more
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