Results 31 to 40 of about 7,683,438 (307)

Multi-Compartment and Multi-Host Vector Suite for Recombinant Protein Expression and Purification

Frontiers in Microbiology, 2018
Recombinant protein expression has become an invaluable tool in basic and applied research. The accumulated knowledge in this field allowed the expression of thousands of protein targets in a soluble, pure, and homogeneous state, essential for ...
Claudia Ortega, Claudia Ortega, Daniel Prieto, Daniel Prieto, Cecilia Abreu, Cecilia Abreu, Pablo Oppezzo, Pablo Oppezzo, Agustín Correa, Agustín Correa   +9 more
doaj   +1 more source

Microfluidic chemostatic bioreactor for high-throughput screening and sustainable co-harvesting of biomass and biodiesel in microalgae

Bioactive Materials, 2023
As a renewable and sustainable source for energy, environment, and biomedical applications, microalgae and microalgal biodiesel have attracted great attention.
Guoxia Zheng, Yutong Cui, Ling Lu, Ming Guo, Xuejun Hu, Lin Wang, Shuping Yu, Shenxia Sun, Yuancheng Li, Xingcai Zhang, Yunhua Wang   +10 more
doaj   +1 more source

Recent advances in CHO cell line development for recombinant protein production.

Drug Discovery Today : Technologies, 2020
Recombinant proteins used in biomedical research, diagnostics and different therapies are mostly produced in Chinese hamster ovary cells in the pharmaceutical industry.
B. Tihanyi, L. Nyitray
semanticscholar   +1 more source

Recombinant plasma proteins [PDF]

Vox Sanguinis, 2010
For almost 50 years, the fractionation of human plasma has been the sole possible source of a wide range of therapeutic proteins–such as coagulation factors, anticoagulants, immunoglobulins, and albumin – essential to the treatment of serious congenital or acquired bleeding or immunological diseases. In the last 20 years, the application of recombinant
openaire   +2 more sources

Therapeutic recombinant protein production in plants: Challenges and opportunities

, 2020
Funding information Biotechnology and Biological Sciences Research Council; Margaret Claire Ryan Fellowship Fund at the Yale Jackson Institute for Global Affairs; U.S.
M. B. Burnett, A. C. Burnett
semanticscholar   +1 more source

Recombinant protein expression in Escherichia coli: advances and challenges

Frontiers in Microbiology, 2014
Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform.
Germán L. Rosano, E. Ceccarelli
semanticscholar   +1 more source

Recombination of Fragmented Proteins [PDF]

Chemistry & Biology, 2004
Directed evolution is a powerful method for generating novel molecules with desirable properties. In developing a new sensor to screen for protein-protein interactions, Tafelmeyer et al. report a clever strategy to evolve heterodimeric "split proteins" from a monomer in this issue of Chemistry & Biology.
openaire   +3 more sources

A protocol for recombinant protein quantification by densitometry

MicrobiologyOpen, 2020
The protein purity is generally checked using SDS‐PAGE, where densitometry could be used to quantify the protein bands. In literature, few studies have been reported using image analysis for the quantification of protein in SDS‐PAGE: that is, imaged with
Susana Maria Alonso villela, Hazar Kraïem, B. Bouhaouala-Zahar, C. Bideaux, César Arturo Aceves Lara, L. Fillaudeau   +5 more
semanticscholar   +1 more source

A Brief Reminder of Systems of Production and Chromatography-Based Recovery of Recombinant Protein Biopharmaceuticals

BioMed Research International, 2019
Recombinant proteins are produced for various applications in laboratory and industrial settings. Among them, therapeutic applications have evolved into a mature field in recent years, affecting the face of contemporary medical treatment.
B. Owczarek, Aneta Gerszberg, K. Hnatuszko-Konka   +2 more
semanticscholar   +1 more source

FASTMAP—a flexible and scalable immunopeptidomics pipeline for HLA- and antigen-specific T-cell epitope mapping based on artificial antigen-presenting cells

Frontiers in Immunology
The study of peptide repertoires presented by major histocompatibility complex (MHC) molecules and the identification of potential T-cell epitopes contribute to a multitude of immunopeptidome-based treatment approaches.
Luisa Weisbrod, Luigi Capriotti, Marco Hofmann, Valerie Spieler, Herbert Dersch, Bernd Voedisch, Peter Schmidt, Susanne Knake   +7 more
doaj   +1 more source