Results 31 to 40 of about 1,320,877 (307)

Protection and Immune Responses Elicited by rSAG1-PLGA Nanoparticles in C57BL/6 Against Toxoplasma gondii [PDF]

open access: yesJournal of Medical Microbiology and Infectious Diseases, 2021
Introduction: This study aimed to evaluate rSAG1-PLGA efficacy as a particulate vaccine in conferring protection against Toxoplasma gondii infection in C57BL/6 mice.
Mojgan Allahyari   +3 more
doaj   +2 more sources

Investigation into the pharmacodynamics and pharmacokinetics of recombinant human interferon alfa-2b vaginal suppository following process optimization in chinese rhesus macaque

open access: yesScientific Reports
Recombinant human interferon Alfa-2b vaginal suppository is a gynecological preparation mainly made of interferon, commonly used to treat diseases related to viral infections such as cervical erosion. As a recombinant protein drug, it is important to pay
Xueyan Zhang   +3 more
doaj   +1 more source

Effective Doses of Recombinant Human Bone Morphogenetic Protein-2 in Experimental Spinal Fusion [PDF]

open access: yes, 1996
Study Design Nineteen dogs underwent L4-L5 intertransverse process fusions with either 58 μg, 115 μg, 230 μg, 460 μg, or 920 μg of recombinant human bone morphogenetic protein-2 carried by a polylactic acid polymer.
Dawson, Edgar G.   +7 more
core   +1 more source

C2238/αANP modulates apolipoprotein E through Egr-1/miR199a in vascular smooth muscle cells in vitro [PDF]

open access: yes, 2015
Subjects carrying the T2238C ANP gene variant have a higher risk to suffer a stroke or myocardial infarction. The mechanisms through which T2238C/αANP exerts detrimental vascular effects need to be fully clarified.
Bianchi, Franca   +9 more
core   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

open access: yesFEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee   +3 more
wiley   +1 more source

Expression of functional recombinant human tissue transglutaminase (TG2) using the bac-to-bac baculovirus expression system [PDF]

open access: yes, 2016
Purpose: Tissue transglutaminase (TG2) is a unique multifunctional enzyme. The enzyme possesses enzymatic activities such as transamidation/crosslinking and non-enzymatic functions such as cell migration and signal transduction.
Azari, S., Kalhor, H.R., Yazdani, Y.
core   +2 more sources

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

open access: yesFEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa   +5 more
wiley   +1 more source

Glycolytic enzymes - novel carbohydrate binding proteins for glycoprotein analysis [PDF]

open access: yes, 2011
•The cloning, expression, purification and characterisation of recombinant prokaryotic glycolytic enzymes •The mutagenesis of prokaryotic glycolytic enzymes to generate novel recombinant carbohydrate binding proteins •The characterisation of the ...
Clarke, Paul A.   +3 more
core  

Recombinant protein expression in biofilms

open access: yesAIMS Microbiology, 2019
Biofilm research is usually focused on the prevention or control of biofilm formation. Recently, the significance of the biofilm mode of growth in biotechnological applications received increased attention. Since biofilm reactors show many advantages over suspended cell reactors, especially in their higher biomass density and operational stability ...
Alexandra Soares   +3 more
openaire   +3 more sources

Recombining DNA by Protein Swivels [PDF]

open access: yesStructure, 2011
Two new reports on serine recombinases, one of a crystal snapshot in an alternate rotational conformer poised for DNA cleavage (Keenholtz et al., 2011), and a second employing single-DNA molecule approaches (Bai et al., 2011), provide strong support for the subunit rotation model for exchanging DNA strands.
Johnson, Reid C., McLean, Meghan M.
openaire   +2 more sources

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