Results 21 to 30 of about 1,320,877 (307)

Recombinant human preproinsulin expression, purification and reaction with insulin autoantibodies in sera from patients with insulin-dependent diabetes mellitus [PDF]

, 1993
A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant fusion protein with a histidine-hexapeptide and glutathione-S-transferase at its N-terminus and the recombinant human preproinsulin at its C-terminus ...
Amman, Atkinson, Baekkeskov, Baekkeskov, Berthold, Boitard, Bottazzo, Castano, Dean, Frorath, Goedel, Greenbaum, Harrison, Heike Berg, Hopp, Jochen Seissler, Karjalainen, Kaufman, Kuglin, Kuglin, Ludwig Mauch, Michael Walter, Palmer, Patrick, Seissler, Smith, Smith, Snyder, Su, Wolfgang Northemann   +29 more
core   +1 more source

Engineering a novel endopeptidase based on SARS 3CLpro

BioTechniques, 2009
A 3C-like protease (3CLpro) from the severe acute respiratory syndrome–coronavirus (SARS-CoV) is required for viral replication, cleaving the replicase polyproteins at 11 sites with the conserved Gln↓(Ser, Ala, Gly) sequences. In this study, we developed
Chih-Jung Kuo, Yan-Ping Shih, Daphne Kan, Po-Huang Liang   +3 more
doaj   +1 more source

19F NMR spectroscopy monitors ligand binding to recombinantly fluorine-labelled b'x from human protein disulphide isomerase (hPDI) [PDF]

, 2014
We report a protein-observe (19)F NMR-based ligand titration binding study of human PDI b'x with ?-somatostatin that also emphasises the need to optimise recombinant protein fluorination when using 5- or 6-fluoroindole.
Byrne, Crowley, Darby, Denisa Doko, Denisov, Fielding, Freedman, Hatahet, Hatahet, Kirsty L. Richards, Klappa, Koivunen, Mark J. Howard, Michelle L. Rowe, Nakasako, Nguyen, Pirneskoski, Richard A. Williamson, Rose Curtis-Marof, Serve, Tian, Wallis, Wang, Wang, Williamson   +24 more
core   +1 more source

Roles of the AtErcc1 protein in recombination [PDF]

The Plant Journal, 2004
SummaryAtercc1, the recently characterized Arabidopsis homologue of the Ercc1 (Rad10) protein, is a key component of nucleotide excision repair as part of a structure‐specific endonuclease which cleaves 5′ to UV photoproducts in DNA. This endonuclease also acts in removing overhanging non‐homologous DNA ‘tails’ in synapsed recombination intermediates ...
Dubest, Sandra, Gallego, Maria, White, Charles   +2 more
openaire   +3 more sources

Microfluidic chemostatic bioreactor for high-throughput screening and sustainable co-harvesting of biomass and biodiesel in microalgae

Bioactive Materials, 2023
As a renewable and sustainable source for energy, environment, and biomedical applications, microalgae and microalgal biodiesel have attracted great attention.
Guoxia Zheng, Yutong Cui, Ling Lu, Ming Guo, Xuejun Hu, Lin Wang, Shuping Yu, Shenxia Sun, Yuancheng Li, Xingcai Zhang, Yunhua Wang   +10 more
doaj   +1 more source

Russell-like bodies in plant seeds share common features with prolamin bodies and occur upon recombinant protein production [PDF]

, 2019
Although many recombinant proteins have been produced in seeds at high yields without adverse effects on the plant, endoplasmic reticulum (ER) stress and aberrant localization of endogenous or recombinant proteins have also been reported.
Arcalis, Elsa, Avesani, Linda, Bortesi, Luisa, De Meyer, Thomas, de meyer, thomas, Depicker, Anna, Hilscher, Julia, Ibl, Verena, Morandini, Francesca, Pezzotti, Mario, Pum, Dietmar, Rademacher, Thomas, Stoger, Eva, Vitale, Alessandro   +13 more
core   +2 more sources

Multi-Compartment and Multi-Host Vector Suite for Recombinant Protein Expression and Purification

Frontiers in Microbiology, 2018
Recombinant protein expression has become an invaluable tool in basic and applied research. The accumulated knowledge in this field allowed the expression of thousands of protein targets in a soluble, pure, and homogeneous state, essential for ...
Claudia Ortega, Claudia Ortega, Daniel Prieto, Daniel Prieto, Cecilia Abreu, Cecilia Abreu, Pablo Oppezzo, Pablo Oppezzo, Agustín Correa, Agustín Correa   +9 more
doaj   +1 more source

Recombinant Protein Stability in Cyanobacteria [PDF]

ACS Synthetic Biology, 2021
The living cell possesses extraordinary molecular and biochemical mechanisms by which to recognize and efficiently remove foreign, damaged, or denatured proteins. This essential function has been a barrier to the overexpression of recombinant proteins in most expression systems. A notable exception is the overexpression in E.
Xianan Zhang, Nico Betterle, Diego Hidalgo Martinez, Anastasios Melis   +3 more
openaire   +5 more sources

Molecular cloning and expression of novel fibroblast growth factor-2 conjugated with immunodominant domains of pseudomonas exotoxin [PDF]

, 2015
Angiogenesis is very important in cancer growth and metastasis. Basic fibroblast growth factor (bFGF) as one of the most important angiogenesis factors is an attractive target for cancer vaccine.
Farazmandfar, T., Haghighatfard, H., Hakemi, M.G., Jadidi-Niaragh, F., Samaei, N.M., Yamchi, A., Yazdani, Y.   +6 more
core   +1 more source

FASTMAP—a flexible and scalable immunopeptidomics pipeline for HLA- and antigen-specific T-cell epitope mapping based on artificial antigen-presenting cells

Frontiers in Immunology
The study of peptide repertoires presented by major histocompatibility complex (MHC) molecules and the identification of potential T-cell epitopes contribute to a multitude of immunopeptidome-based treatment approaches.
Luisa Weisbrod, Luigi Capriotti, Marco Hofmann, Valerie Spieler, Herbert Dersch, Bernd Voedisch, Peter Schmidt, Susanne Knake   +7 more
doaj   +1 more source