Frontiers in Bioengineering and Biotechnology, 2022 Recombinant therapeutic proteins (RTPs) are important parts of biopharmaceuticals. Chinese hamster ovary cells (CHO) have become the main cell hosts for the production of most RTPs approved for marketing because of their high-density suspension growth ...
Zheng-Mei Li +7 more
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Advances of Glycometabolism Engineering in Chinese Hamster Ovary Cells
Frontiers in Bioengineering and Biotechnology, 2021 As the most widely used mammalian cell line, Chinese hamster ovary (CHO) cells can express various recombinant proteins with a post translational modification pattern similar to that of the proteins from human cells.
Huan-Yu Zhang +5 more
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Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells
Frontiers in Bioengineering and Biotechnology, 2021 At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to produce RTPs.
Weifeng Li +4 more
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The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism
Frontiers in Bioengineering and Biotechnology, 2022 Recombinant protein production by mammalian cells is the initial step in the manufacture of many therapeutic proteins. Chinese hamster ovary (CHO) cells are the most common host system to produce recombinant therapeutic proteins (RTPs).
Hui-Ning Liu +7 more
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Maltose-binding protein enhances secretion of recombinant human granzyme B accompanied by in vivo processing of a precursor MBP fusion protein [PDF]
, 2010 Background: The apoptosis-inducing serine protease granzyme B (GrB) is an important factor contributing to lysis of target cells by cytotoxic lymphocytes.
Wels, Winfried +9 more
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Screening Strategies for High-Yield Chinese Hamster Ovary Cell Clones
Frontiers in Bioengineering and Biotechnology, 2022 Chinese hamster ovary (CHO) cells are by far the most commonly used mammalian expression system for recombinant expression of therapeutic proteins in the pharmaceutical industry.
Wenwen Yang +8 more
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Improvement of downstream processing of recombinant proteins by means of genetic engineering [PDF]
, 1993 Flaschel E, Friehs K. Improvement of downstream processing of recombinant proteins by means of genetic engineering. Biotechnology Advances. 1993;11(1):31-77.The rapid advancement of genetic engineering has allowed to produce an impressive number of ...
Flaschel, Erwin, Friehs, Karl
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An outlook on coronavirus disease 2019 detection methods
Journal of Pharmaceutical Analysis, 2022 Diagnostic testing plays a fundamental role in the mitigation and containment of coronavirus disease 2019 (COVID-19), as it enables immediate quarantine of those who are infected and contagious and is essential for the epidemiological characterization of
Zahra Hassani Nejad +2 more
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Expression of recombinant multi-coloured fluorescent antibodies in
, 2011 Background Antibody-fluorophore conjugates are invaluable reagents used in contemporary molecular cell biology for imaging, cell sorting and tracking intracellular events.
Durvasula, Ravi V. +26 more
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Engineering a novel endopeptidase based on SARS 3CLpro
BioTechniques, 2009 A 3C-like protease (3CLpro) from the severe acute respiratory syndrome–coronavirus (SARS-CoV) is required for viral replication, cleaving the replicase polyproteins at 11 sites with the conserved Gln↓(Ser, Ala, Gly) sequences. In this study, we developed
Chih-Jung Kuo +3 more
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