Results 1 to 10 of about 228,451 (311)

Retrovectors packaged in CHO cells to generate GLP-1-Fc stable expression CHO cell lines

Electronic Journal of Biotechnology, 2019
Background: Chinese hamster ovary (CHO) cells are the most dependable mammalian cells for the production of recombinant proteins. Replication-incompetent retroviral vector (retrovector) is an efficient tool to generate stable cell lines.
Jing Li, Suzhen Wei, Chunlai Cao, Kangyue Chen, Hua He, Guoquan Gao   +5 more
doaj   +3 more sources

Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells [PDF]

Scientific Reports
To meet the requirements of the biopharmaceutical industry, improving the yield of recombination therapeutic protein (RTP) from Chinese hamster ovary (CHO) cells is necessary.
Ting-Ting Xu, Yan-Fang Wang, Jing-Jia Yuan, Chun-Liu Mi, Shao-Lei Geng, Xiao-yin Wang, Tian-Yun Wang   +6 more
doaj   +2 more sources

Epigenetic regulation of histone methyltransferase SUV39H1 on the expression of recombinant protein in CHO cells [PDF]

Applied Microbiology and Biotechnology
Abstract Histone methylation–mediated epigenetic modification significantly influences gene transcription and expression regulation. This study examined the effects of histone 3 lysine 9 trimethylation (H3K9me3) methyltransferase SUV39H1 and its specific
Lu-Lu Yang, Miao Zhang, Jia-Liang Guo, Ming-Ming Han, Le-Le Qiu, Li-Jie Gao, Liu-Su Cui, Xiao-Yin Wang, Tian-Yun Wang, Yan-Long Jia   +9 more
doaj   +2 more sources

An Easy And Fast Method For Production Of Chinese Hamster Ovary Cell Line Expressing And Secreting Human Recombinant Activin A [PDF]

Cell Journal, 2020
Objective Growth factors are key elements of embryonic stem cell (ESC) research. Cell line development in eukaryotes is a time-consuming procedure which usually takes 12-18 months. Here, we report an easy and fast method with which production of Chinese
Hassan Rassouli, Ali Sayadmanesh, Siamak Rezaeiani, Zahra Ghezelayagh, Mohammad Reza Gharaati, Tahamtani Yaser   +5 more
doaj   +1 more source

Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice

Life, 2022
Angiogenesis is a process to generate new blood vessels from pre-existing vessels and to maintain vessels, and plays critical roles in normal development and disease.
Chisato Watanabe, Hirotoshi Shibuya, Yusuke Ichiyama, Eiichi Okamura, Setsuko Tsukiyama-Fujii, Tomoyuki Tsukiyama, Shoma Matsumoto, Jun Matsushita, Takuya Azami, Yoshiaki Kubota, Masahito Ohji, Fumihiro Sugiyama, Satoru Takahashi, Seiya Mizuno, Masaru Tamura, Ken-ichi Mizutani, Masatsugu Ema   +16 more
doaj   +1 more source

Production of Monoclonal Antibody Anti-NS1 Induced by DENV3 Indonesian Clinical Isolate to Detect Native NS1 Antigen of all DENV Serotypes [PDF]

Journal of Clinical and Diagnostic Research, 2018
Introduction: Dengue Virus (DENV) infection is a mosquito-borne disease, the most severe manifestation of the infection could lead to a lethal Dengue Haemorrhagic Fever (DHF) and/or Dengue Shock Syndrome (DSS).
Tika Widayanti, Beti Ernawati Dewi, Sabar Pambudi   +2 more
doaj   +1 more source

Comparison of Human Factor IX Abundance and mRNA Expression Levels In Stable Insect and Mammalian Cells [PDF]

Journal of Cell and Molecular Research, 2017
In the production of recombinant proteins, the selection of an expression system is very important. Although CHO cells are used as mammalian expression system, the ability of insect Schneider line 2 (S2) cells as a new expression system for the high ...
Jafar Vatandoost, Shadi Tirdad
doaj   +1 more source

Caspase-7 deficiency in Chinese hamster ovary cells reduces cell proliferation and viability

Biological Research, 2020
Background Chinese hamster ovary (CHO) cells are the most commonly used mammalian host cell in the commercial-scale production of biopharmaceutical proteins. Modification of genes involved in apoptosis may improve the productivity of CHO cells. Executive
Fatemeh Safari, Safar Farajnia, Abbas Behzad Behbahani, Habib Zarredar, Mazyar Barekati-Mowahed, Hesam Dehghani   +5 more
doaj   +1 more source

Establishment of a novel anti-TROP2 monoclonal antibody TrMab-29 for immunohistochemical analysis

Biochemistry and Biophysics Reports, 2021
TROP2 is a type I transmembrane glycoprotein originally identified in human trophoblast cells that is overexpressed in several types of cancer. To better understand the role of TROP2 in cancer, we herein aimed to develop a sensitive and specific anti ...
Yusuke Sayama, Mika K. Kaneko, Junko Takei, Hideki Hosono, Masato Sano, Teizo Asano, Yukinari Kato   +6 more
doaj   +1 more source

Towards Tumor Targeting via Invasive Assay Using Magnetospirillum magneticum

Frontiers in Microbiology, 2021
Magnetospirillum magneticum (AMB-1) are a species of magnetotactic bacteria (MTB) that are capable of orienting along the earth’s magnetic field lines through their organelles called magnetosomes.
Marvin Xavierselvan, Heena R. Divecha, Mamta Hajra, Sushila Silwal, Isaac Macwan   +4 more
doaj   +1 more source