Results 41 to 50 of about 228,451 (311)
Bradykinin release avoids high molecular weight kininogen endocytosis. [PDF]
PLoS ONE, 2015 Human H-kininogen (120 kDa) plays a role in many pathophysiological processes and interacts with the cell surface through protein receptors and proteoglycans, which mediate H-kininogen endocytosis.
Igor Z Damasceno +9 more
doaj +1 more source
Awakening dormant glycosyltransferases in CHO cells with CRISPRa [PDF]
Biotechnology and Bioengineering, 2019 AbstractChinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells. Here, we expand the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes.
Karen Julie la Cour Karottki +11 more
openaire +4 more sources
Cell fusion of EnvΔR-expressing CHO cells. [PDF]
, 2016 Lentiviral vectors encoding ecotropic (a) or amphotropic (b) EnvΔR-IRES-EGFP were used to infect CHO cells (green, upper panels), which were then co-cultured with CHO, NIH3T3, or 293T cells labeled with TdTomato (red, middle panels).
Takahiko Hara (365321) +3 more
core +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
Cell-cell fusion activities of XMRV Env and mutants in CHO or CHO cells expressing XPR1. [PDF]
, 2013 (A) Immunostaining of CHO and CHO/XPR1 cells. Cells were fixed, permeabilized, stained for XPR1 using anti-XPR1 and FITC-coupled secondary antibodies, and counterstained using DAPI.
Marceline Côté (328257) +2 more
core +1 more source
Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis
FEBS Letters, EarlyView.Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee +7 more
wiley +1 more source
Тонкие химические технологииObjectives. To develop an effective technology for the cultivation of Chinese hamster ovary (CHO) cells stably producing GamP2C5 antibody which is a component I of the GamCoviMab candidate drug for emergency prevention and therapy of infection caused by
D. S. Polyansky +7 more
doaj +1 more source
NPC2 protein expression in CHO cells. [PDF]
, 2013 Immunohistochemical stain of NPC2 protein in wild CHO cell (a), CHO cells stably transfected with NPC2 (b) and xenograft of CHO cells transfected with NPC2 (c). No papilla was seen in the xenograft, despite abundant NPC2 protein expression.
Farhad Moatamed (146749) +6 more
core +1 more source
Molecular Oncology, EarlyView.Glioma cells mainly express the endothelin receptor EDNRB, while EDNRA is restricted to a perivascular tumor subpopulation. Endothelin signaling reduces glioma cell proliferation while promoting migration and a proneural‐to‐mesenchymal transition associated with poor prognosis. This pathway activates Ca2+, K+, ERK, and STAT3 signalings and is regulated
Donovan Pineau +36 more
wiley +1 more source
Solid Free-form Fabrication Technology and its Application to Bone Tissue Engineering [PDF]
, 2010 11scopu
Jong Young Kim +2 more
core +1 more source