Results 31 to 40 of about 12,852,402 (297)

A theoretical estimate for nucleotide sugar demand towards Chinese Hamster Ovary cellular glycosylation [PDF]

, 2016
Glycosylation greatly influences the safety and efficacy of many of the highest-selling recombinant therapeutic proteins (rTPs). In order to define optimal cell culture feeding strategies that control rTP glycosylation, it is necessary to know how ...
Jimenez del Val, I, Kontoravdi, C, Polizzi, K   +2 more
core   +1 more source

USING OF UNIVERSAL PLASMID CONSTRUCTIONS FOR DESIGN OF RECOMBINANT ANTIBODIES WITH DEFINED SPECIFICITY IN EUKARYOTIC CELLS

Журнал микробиологии, эпидемиологии и иммунобиологии, 2019
Aim. In this study we aimed to develop the methodology to change the antigen specificity of chimeric antibodies by replacing the variable region genes in the previously designed universal plasmid constructions pLK DT-17 and pHG DT-17 encoding the DT-17 ...
T. G. Samartseva, A. S. Oksanich, N. F. Gavrilova, I. V. Yakovleva, V. V. Sviridov, V. V. Zverev   +5 more
doaj   +1 more source

High-Level Expression of Various Apolipoprotein (a) Isoforms by "Transferrinfection". The Role of Kringle IV Sequences in the Extracellular Association with Low-Density Lipoprotein [PDF]

, 1994
Characterization of the assembly of lipoprotein(a) [Lp(a)] is of fundamental importance to understanding the biosynthesis and metabolism of this atherogenic lipoprotein. Since no established cell lines exist that express Lp(a) or apolipoprotein(a) [apo(a)
Azrolan N., Boerwinkle E., Brunner C., Chiesa G., Chung B. Y., Cotten M., Deutscher S. L., Deutscher S. L., Dieplinger H., Edelberg J. M., Green S., Griffiths G., Guevara J., Jr., Huby T., Ichinose A., Kamboh M. I., Koschinsky M. L., Koschinsky M. L., Koschinsky M. L., Kostner G. M., Kraft H- G., Kraft H. G., Krempler F., Krempler F., Lackner C., Linton M. F., Malgaretti N., McGregor G. R., McLean J. W., Menzel H. J., Phillips M. L., Rader D. J., Sambrook J., Sandholzer C., Scanu A. M., Selden R. F., Sommer A., Stanley P., Trieu V. N., Utermann G., Utermann G., Utermann G., Wagner E., White A. L., Zatloukal K., Zechner R„   +45 more
core   +1 more source

CD300LG improves the cytotoxic activity of CIK

Central European Journal of Immunology, 2017
To investigate the effect of CD300LG-γ induction on the cytotoxic activity of CIK. Eukaryotic expression plasmid hCD300LG-γ/pEGFP-C3, which can express human CD300LG-γ, was constructed and transfected into CHO cells by lipofectamine.
Qiuyue Wang, Yuqing Liu, Ying Chen, Ke Wang, Wensheng Xie, Dapeng Wei, Lijuan Hu   +6 more
doaj   +1 more source

Glycoproteomic Characterization of FUT8 Knock-Out CHO Cells Reveals Roles of FUT8 in the Glycosylation

Frontiers in Chemistry, 2021
The α1,6-fucosyltransferase (encoded by FUT8 gene) is the key enzyme transferring fucose to the innermost GlcNAc residue on an N-glycan through an α-1,6 linkage in the mammalian cells.
Ganglong Yang, Qiong Wang, Lijun Chen, Michael J. Betenbaugh, Hui Zhang, Hui Zhang   +5 more
doaj   +1 more source

Histamine H1 Receptor Down-Regulation Mediated by M3 Muscarinic Acetylcholine Receptor Subtype

Journal of Pharmacological Sciences, 2004
Heterologous down-regulation of histamine H1 receptor (H1R) mediated by muscarinic acetylcholine receptor subtype was investigated using five kinds of Chinese hamster ovary (CHO) cells stably co-expressing the human H1R and one of the five (M1 –M5 ...
Katsuhiro Miyoshi, Nozomi Kawakami, Yousuke Wakayama, Norimasa Izumi, Shuhei Horio, Hiroyuki Fukui   +5 more
doaj   +1 more source

Enzyme capacity-based genome scale modelling of CHO cells

bioRxiv, 2019
Chinese hamster ovary (CHO) cells are most prevalently used for producing recombinant therapeutics in biomanufacturing. Recently, more rational and systems approaches have been increasingly exploited to identify key metabolic bottlenecks and engineering ...
Hock Chuan Yeo, Jongkwang Hong, Meiyappan Lakshmanan, Dong-Yup Lee   +3 more
semanticscholar   +1 more source

Cell Propagation of Cholera Toxin CTA ADP-Ribosylating Factor by Exosome Mediated Transfer [PDF]

, 2018
In this study, we report how the cholera toxin (CT) A subunit (CTA), the enzyme moiety responsible for signaling alteration in host cells, enters the exosomal pathway, secretes extracellularly, transmits itself to a cell population.
Boussadia, Zaira, Carollo, Maria, Fabbri, Alessia, Falchi, Mario, Fecchi, Katia, Fiani, Maria Luisa, Gallina, Angelo, Palermo, Angela, Parisi, Sofia, Pasquini, Luca, Sargiacomo, Massimo, Zanetti, Cristiana   +11 more
core   +1 more source

A metabolic network-based approach for developing feeding strategies for CHO cells to increase monoclonal antibody production

bioRxiv, 2019
Chinese hamster ovary (CHO) cells are the main workhorse in the biopharmaceutical industry for the production of recombinant proteins, such as monoclonal antibodies.
Hamideh Fouladiha, S. Marashi, F. Torkashvand, F. Mahboudi, N. Lewis, B. Vaziri   +5 more
semanticscholar   +1 more source

The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism

Frontiers in Bioengineering and Biotechnology, 2022
Recombinant protein production by mammalian cells is the initial step in the manufacture of many therapeutic proteins. Chinese hamster ovary (CHO) cells are the most common host system to produce recombinant therapeutic proteins (RTPs).
Hui-Ning Liu, Hui-Ning Liu, Wei-Hua Dong, Wei-Hua Dong, Yan Lin, Zhao-Hui Zhang, Tian-Yun Wang, Tian-Yun Wang   +7 more
doaj   +1 more source