Correction of the neuropathogenic human apolipoprotein E4 (APOE4) gene to APOE3 in vitro using synthetic RNA/DNA oligonucleotides (chimeraplasts) [PDF]
, 2005 Apolipoprotein E (apoE) is a multifunctional circulating 34-kDa protein, whose gene encodes single-nucleotide polymorphisms linked to several neurodegenerative diseases.
Dickson, JG +3 more
core +1 more source
Cloning of CHO Cells, Productivity and Genetic Stability—A Discussion
, 2017 While many perceive mammalian cell culture-based manufacturing for biopharmaceuticals an established technology, numerous open questions remain to be solved.
F. Wurm, Maria J. Wurm
semanticscholar +1 more source
Metabolic engineering of CHO cells for the development of a robust protein production platform
PLoS ONE, 2017 Chinese hamster ovary (CHO) cells are the most preferred mammalian host used for the bio-pharmaceutical production. A major challenge in metabolic engineering is to balance the flux of the tuned heterogonous metabolic pathway and achieve efficient ...
Sanjeev Gupta +7 more
semanticscholar +1 more source
The Era of Gene Therapy: The Advancement of Lentiviral Vectors and Their Pseudotyping
VirusesOver 35 years of history, the field of gene therapy has undergone much progress. The initial concept—the replacement of dysfunctional genes with correct ones—has advanced to the next stage and reached the level of precise genome editing.
Bat-Erdene Jargalsaikhan +2 more
doaj +1 more source
Modulation of the Plasma Kallikrein-Kinin System Proteins Performed by Heparan Sulfate Proteoglycans
Frontiers in Physiology, 2017 Human plasma kallikrein-kinin system proteins are related to inflammation through bradykinin. In the proximity of its target cells, high molecular weight kininogen (H-kininogen) is the substrate of plasma kallikrein, which releases bradykinin from H ...
Guacyara Motta, Ivarne L. S. Tersariol
doaj +1 more source
Biological Research, 2022 Background Chinese hamster ovary cell line has been used routinely as a bioproduction factory of numerous biopharmaceuticals. So far, various engineering strategies have been recruited to improve the production efficiency of this cell line such as ...
Fatemeh Safari, Bahman Akbari
doaj +1 more source
A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins [PDF]
, 2001 Fucans, a family of sulfated polysaccharides present in brown seaweed, have several biological activities. Their use as drugs would offer the advantage of no potential risk of contamination with viruses or particles such as prions.
Carvalho, L.c.m. +7 more
core +2 more sources
The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells
Nature Communications, 2019 Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution.
Weihua Tian +17 more
semanticscholar +1 more source
Biotechnology and Bioengineering, 2016 The most striking characteristic of CHO cells is their adaptability, which enables efficient production of proteins as well as growth under a variety of culture conditions, but also results in genomic and phenotypic instability.
J. Feichtinger +18 more
semanticscholar +1 more source