Bioreactor cultivation of CHO DP-12 cells under sodium butyrate treatment – comparative transcriptome analysis with CHO cDNA microarrays [PDF]
, 2011 Klausing S, Krämer O, Noll T. Bioreactor cultivation of CHO DP-12 cells under sodium butyrate treatment – comparative transcriptome analysis with CHO cDNA microarrays. BMC Proceedings. 2011;5(Suppl 8): P98
Sandra Klausing +2 more
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GRP78 protects CHO cells from ribosylation
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2018 d-Ribose (Rib), a reactive glycation compound that exists in organisms, abnormally increases in the urine of diabetic patients and can yield large amounts of advanced glycation end products (AGEs), leading to cell dysfunction. However, whether cellular proteins are sensitive to this type of glycation is unknown.
Wu, Beibei +8 more
openaire +3 more sources
Amplification Using
Current Protocols in Molecular Biology, 2002 AbstractThe ability to select for integration of plasmid DNA into the host chromosome allows the generation of stably transfected cell lines. With transfection of a selectable marker linked to a nonselectable target gene (or by cotransfection of the two unlinked genes), high‐level expression of the desired gene is obtained by selecting for ...
Kingston, Robert E. +3 more
openaire +3 more sources
Scientific Reports, 2018 To characterize a glutamine synthetase (GS)-based selection system, monoclonal antibody (mAb) producing recombinant CHO cell clones were generated by a single round of selection at various methionine sulfoximine (MSX) concentrations (0, 25, and 50 μM ...
S. Noh, Seunghyeon Shin, G. Lee
semanticscholar +1 more source
Bradykinin release avoids high molecular weight kininogen endocytosis. [PDF]
PLoS ONE, 2015 Human H-kininogen (120 kDa) plays a role in many pathophysiological processes and interacts with the cell surface through protein receptors and proteoglycans, which mediate H-kininogen endocytosis.
Igor Z Damasceno +9 more
doaj +1 more source
Recombinant Vgr-1/BMP-6-expressing tumors induce fibrosis and endochondral bone formation in vivo. [PDF]
, 1994 Members of the TGF-beta superfamily appear to modulate mesenchymal differentiation, including the processes of cartilage and bone formation. Nothing is yet known about the function of the TGF-beta-related factor vgr-1, also called bone morphogenetic ...
Derynck, R +5 more
core
Truncated human endothelin receptor A produced by alternative splicing and its expression in melanoma [PDF]
, 1998 In this study, reverse transcriptase polymerase chain reaction was used to amplify human endothelin receptor A (ETA) and ETB receptor mRNA. A truncated ETA receptor transcript with exons 3 and 4 skipped was found.
Berry, PA +5 more
core +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
The polarized expression of Na+,K+-ATPase in epithelia depends on the association between beta-subunits located in neighboring cells [PDF]
, 2005 The polarized distribution of Na+,K+-ATPase plays a paramount physiological role, because either directly or through coupling with co- and countertransporters, it is responsible for the net movement of, for example, glucose, amino acids, Ca2+, K+, Cl ...
Balda, MS +7 more
core
Binding of Plasmodium falciparum to CD36 can be shielded by the glycocalyx [PDF]
, 2017 Background Plasmodium falciparum-infected erythrocytes sequester in the microcirculation due to interaction between surface-expressed parasite proteins and endothelial receptors.
Hempel, Casper +3 more
core +3 more sources