Results 21 to 30 of about 228,451 (311)
Optimization of human interferon beta protein expression in Chinese hamster ovary cells [PDF]
Journal of Shahrekord University of Medical Sciences, 2021 Background and aims: Human interferon beta-1a (hIFNβ-1a) is a 22.5-kDa glycoprotein used to treat diseases such as multiple sclerosis (MS). Because of appropriate post-translation modifications, protein isolation, and lack of toxicity in Chinese hamster ...
Nasrin Xodadadi +3 more
doaj +1 more source
Regulated autocrine growth of CHO cells [PDF]
Cytotechnology, 2000 The goal of this work was to engineer a CHO cell line capable ofautocrine growth in a fully defined protein-free medium. Thiswas accomplished by stable integration of the genes encodinginsulin-like growth factor I (IGF-I) and transferrin into thegenome of a CHO-K1 cell line. Thelac operator/repressorsystem was used to regulate the expression of the IGF-
N A, Sunstrom +6 more
openaire +2 more sources
Separation of CHO cells using hydrocyclones [PDF]
Cytotechnology, 2007 Hydrocyclones are simple and robust separation devices with no moving parts. In the past few years, their use in animal cell separation has been proposed. In this work, the use of different hydrocyclone configurations for Chinese hamster ovary (CHO) cell separation was investigated following an experimental design.
Rodrigo C V, Pinto +2 more
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High Throughput miRNA Screening Identifies miR-574-3p Hyperproductive Effect in CHO Cells
Biomolecules, 2021 CHO is the cell line of choice for the manufacturing of many complex biotherapeutics. The constant upgrading of cell productivity is needed to meet the growing demand for these life-saving drugs.
Živa Švab +7 more
doaj +1 more source
Role of Ku80-dependent end-joining in delayed genomic instability in mammalian cells surviving ionizing radiation. [PDF]
, 2010 Ionizing radiation induces delayed destabilization of the genome in the progenies of surviving cells. This phenomenon, which is called radiation-induced genomic instability, is manifested by delayed induction of radiation effects, such as cell death ...
Suzuki, Keiji +2 more
core +1 more source
The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism
Frontiers in Bioengineering and Biotechnology, 2022 Recombinant protein production by mammalian cells is the initial step in the manufacture of many therapeutic proteins. Chinese hamster ovary (CHO) cells are the most common host system to produce recombinant therapeutic proteins (RTPs).
Hui-Ning Liu +7 more
doaj +1 more source
Characterisation of hepcidin response to holotransferrin treatment in CHO TRVb-1 cells [PDF]
, 2015 Iron overload coupled with low hepcidin levels are characteristics of hereditary haemochromatosis. To understand the role of transferrin receptor (TFR) and intracellular iron in hepcidin secretion, Chinese hamster ovary transferrin receptor variant (CHO ...
Garcia, M +22 more
core +1 more source
CHO Lec8 cells lack DCIR binding glycans. [PDF]
, 2013 (A) DCIR binding to CHO cells. Binding of DCIR-Fc beads to CHO and CHO Lec8 cells was measured by flow cytometry. Percentage of cells that bound the CLR-Fc beads is depicted. One representative experiment out of 6 is shown.
Johan Garssen (278720) +7 more
core +1 more source
PLoS ONE, 2012 Cell microparticles (MPs) released in the extracellular milieu can embark plasma membrane and intracellular components which are specific of their cellular origin, and transfer them to target cells.
Gaëlle Gonzalez +5 more
doaj +1 more source
Glycoengineering in CHO Cells: Advances in Systems Biology [PDF]
Biotechnology Journal, 2018 For several decades, glycoprotein biologics have been successfully produced from Chinese hamster ovary (CHO) cells. The therapeutic efficacy and potency of glycoprotein biologics are often dictated by their post‐translational modifications, particularly glycosylation, which unlike protein synthesis, is a non‐templated process. Consequently, both native
Vijay Tejwani +3 more
openaire +2 more sources