Improving recombinant protein production in CHO cells using the CRISPR-Cas system
Biotechnology Advances, 2023 Chinese hamster ovary (CHO) cells are among the most widely used mammalian cell lines in the biopharmaceutical industry. Therefore, it is not surprising that significant efforts have been made around the engineering of CHO cells using genetic engineering
Ali Kerem Kalkan, Fahreddin Palaz
exaly +2 more sources
Heparan sulfate and control of cell division: adhesion and proliferation of mutant CHO-745 cells lacking xylosyl transferase [PDF]
Brazilian Journal of Medical and Biological Research, 2001 We have examined the role of cell surface glycosaminoglycans in cell division: adhesion and proliferation of Chinese hamster ovary (CHO) cells.
C.R.C. Franco +7 more
doaj +3 more sources
Retrovectors packaged in CHO cells to generate GLP-1-Fc stable expression CHO cell lines
Electronic Journal of Biotechnology, 2019 Background: Chinese hamster ovary (CHO) cells are the most dependable mammalian cells for the production of recombinant proteins. Replication-incompetent retroviral vector (retrovector) is an efficient tool to generate stable cell lines.
Jing Li +5 more
doaj +3 more sources
Glycoengineering in CHO Cells: Advances in Systems Biology [PDF]
Biotechnology Journal, 2018 For several decades, glycoprotein biologics have been successfully produced from Chinese hamster ovary (CHO) cells. The therapeutic efficacy and potency of glycoprotein biologics are often dictated by their post‐translational modifications, particularly glycosylation, which unlike protein synthesis, is a non‐templated process. Consequently, both native
Vijay Tejwani +3 more
openaire +4 more sources
The GalNAc-type O-Glycoproteome of CHO Cells Characterized by the SimpleCell Strategy [PDF]
Molecular & Cellular Proteomics, 2014 The Chinese hamster ovary cell (CHO) is the major host cell factory for recombinant production of biological therapeutics primarily because of its “human-like” glycosylation features.
Alder Schulz, Morten +12 more
core +2 more sources
An Easy And Fast Method For Production Of Chinese Hamster Ovary Cell Line Expressing And Secreting Human Recombinant Activin A [PDF]
Cell Journal, 2020 Objective Growth factors are key elements of embryonic stem cell (ESC) research. Cell line development in eukaryotes is a time-consuming procedure which usually takes 12-18 months. Here, we report an easy and fast method with which production of Chinese
Hassan Rassouli +5 more
doaj +1 more source
Progress in fed-batch culture for recombinant protein production in CHO cells
Applied Microbiology and Biotechnology, 2023 Nearly 80% of the approved human therapeutic antibodies are produced by Chinese Hamster Ovary (CHO) cells. To achieve better cell growth and high-yield recombinant protein, fed-batch culture is typically used for recombinant protein production in CHO ...
Wen-Jing Xu +4 more
semanticscholar +1 more source
Metabolic Profiling of CHO Cells during the Production of Biotherapeutics
Cells, 2022 As indicated by an ever-increasing number of FDA approvals, biotherapeutics constitute powerful tools for the treatment of various diseases, with monoclonal antibodies (mAbs) accounting for more than 50% of newly approved drugs between 2014 and 2018 ...
Mathilde Coulet +3 more
semanticscholar +1 more source
Production of Monoclonal Antibody Anti-NS1 Induced by DENV3 Indonesian Clinical Isolate to Detect Native NS1 Antigen of all DENV Serotypes [PDF]
Journal of Clinical and Diagnostic Research, 2018 Introduction: Dengue Virus (DENV) infection is a mosquito-borne disease, the most severe manifestation of the infection could lead to a lethal Dengue Haemorrhagic Fever (DHF) and/or Dengue Shock Syndrome (DSS).
Tika Widayanti +2 more
doaj +1 more source