Results 11 to 20 of about 4,711,608 (247)
Adaptation to suspension growth: analysing the surface of suspension growth adapted Chinese hamster ovary cells [PDF]
, 2013 Many bio-pharmaceutical production processes are based upon the use of mammalian cell lines, such as Chinese hamster ovary (CHO) cells, capable of proliferation as single cells in suspension in a synthetic environment.
Walther, Christa G
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A Mechanistic Dissection of Polyethylenimine Mediated Transfection of Chinese Hamster Ovary Cells [PDF]
, 2013 Biopharmaceutical production through transient gene expression (TGE) is used within industry for the rapid supply of product for early stage testing.
Mozley, O L
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CHO microRNA engineering is growing up : recent successes and future challenges [PDF]
, 2013 microRNAs with their ability to regulate complex pathways that control cellular behavior and phenotype have been proposed as potential targets for cell engineering in the context of optimization of biopharmaceutical production cell lines, specifically of
Grillari, Johannes +12 more
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1H NMR Spectroscopy Profiling of Metabolic Reprogramming of Chinese Hamster Ovary Cells upon a Temperature Shift during Culture [PDF]
, 2013 We report an NMR based approach to determine the metabolic reprogramming of Chinese hamster ovary cells upon a temperature shift during culture by investigating the extracellular cell culture media and intracellular metabolome of CHOK1 and CHO-S cells ...
Mark J. Howard (443860) +14 more
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Журнал микробиологии, эпидемиологии и иммунобиологии, 2019 Aim. In this study we aimed to develop the methodology to change the antigen specificity of chimeric antibodies by replacing the variable region genes in the previously designed universal plasmid constructions pLK DT-17 and pHG DT-17 encoding the DT-17 ...
T. G. Samartseva +5 more
doaj +1 more source
Frontiers in Chemistry, 2021 The α1,6-fucosyltransferase (encoded by FUT8 gene) is the key enzyme transferring fucose to the innermost GlcNAc residue on an N-glycan through an α-1,6 linkage in the mammalian cells.
Ganglong Yang +5 more
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CD300LG improves the cytotoxic activity of CIK
Central European Journal of Immunology, 2017 To investigate the effect of CD300LG-γ induction on the cytotoxic activity of CIK. Eukaryotic expression plasmid hCD300LG-γ/pEGFP-C3, which can express human CD300LG-γ, was constructed and transfected into CHO cells by lipofectamine.
Qiuyue Wang +6 more
doaj +1 more source
Histamine H1 Receptor Down-Regulation Mediated by M3 Muscarinic Acetylcholine Receptor Subtype
Journal of Pharmacological Sciences, 2004 Heterologous down-regulation of histamine H1 receptor (H1R) mediated by muscarinic acetylcholine receptor subtype was investigated using five kinds of Chinese hamster ovary (CHO) cells stably co-expressing the human H1R and one of the five (M1 –M5 ...
Katsuhiro Miyoshi +5 more
doaj +1 more source
Regulated autocrine growth of CHO cells [PDF]
Cytotechnology, 2000 The goal of this work was to engineer a CHO cell line capable ofautocrine growth in a fully defined protein-free medium. Thiswas accomplished by stable integration of the genes encodinginsulin-like growth factor I (IGF-I) and transferrin into thegenome of a CHO-K1 cell line. Thelac operator/repressorsystem was used to regulate the expression of the IGF-
N A, Sunstrom +6 more
openaire +2 more sources
The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism
Frontiers in Bioengineering and Biotechnology, 2022 Recombinant protein production by mammalian cells is the initial step in the manufacture of many therapeutic proteins. Chinese hamster ovary (CHO) cells are the most common host system to produce recombinant therapeutic proteins (RTPs).
Hui-Ning Liu +7 more
doaj +1 more source