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Recombinase polymerase amplification in minimally buffered conditions

open access: yesBiosensors and Bioelectronics, 2022
Application of recombinase polymerase amplification (RPA) for pH-based detection of DNA amplification has been investigated. Commercial RPA kits from TwistDx are modified to minimize their pH buffering capacity. Due to the RPA's unique biochemistry, removal of tris from the amplification kit is not enough to lower the buffering capacity of the RPA ...
Carlotta Guiducci   +2 more
exaly   +3 more sources

Solvent engineering studies on recombinase polymerase amplification [PDF]

open access: yesJournal of Bioscience and Bioengineering, 2021
Recombinase polymerase amplification (RPA) is a technique that is used to specifically amplify a target nucleic acid sequence. Unlike the polymerase chain reaction (PCR), RPA is performed at a constant temperature between 37 and 42°C. Therefore, it can be potentially used for the onsite detection of various pathogens when combined with DNA extraction ...
Kiyoshi Yasukawa   +2 more
exaly   +3 more sources
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การตรวจไวรัสมะเร็งเม็ดเลือดขาวในแมวที่รวดเร็วด้วยวิธี recombinase polymerase amplification (RPA)

2023
โรคติดเชื้อไวรัสมะเร็งเม็ดเลือดขาวในแมว (Feline Leukemia Virus : FeLV) เป็นโรคติดเชื้อไวรัสในแมวที่สำคัญที่เป็นสาเหตุของโรคมะเร็งเม็ดเลือดขาว และ มะเร็งต่อมน้ำเหลือง การตรวจหา FeLV RNA  และโปรไวรัส ด้วยวิธีทางอณูชีววิทยามีความสำคัญในการแยกระยะต่างๆของโรค ซึ่งวิธี Rapid immunochromatographic assay ที่นิยมใช้ตรวจแอนติเจนนั้นสามารถตรวจได้เพียงการติดเช ...
openaire   +1 more source

A recombinase polymerase amplification assay for the diagnosis of atypical pneumonia

Analytical Biochemistry, 2018
Pneumonia is one of the most common and potentially lethal infectious conditions worldwide. Streptococcus pneumoniae is the pathogen most frequently associated with bacterial community-acquired pneumonia, while Legionella pneumophila is the major cause for local outbreaks of legionellosis.
Kersting, Sebastian   +3 more
openaire   +2 more sources

Recombinase Polymerase Amplification

2020
Alangar Ishwara Bhat, Govind Pratap Rao
openaire   +1 more source

Rapid detection of (DIV1) by recombinase polymerase amplification

Journal of Virological Methods, 2022
Guanghua Huang, Xinxian Wei
exaly  

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