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Biochemistry
Homologous recombination is a vital biological process for DNA repair, genomic stability, and genetic diversity, driven by the RecA/Rad51 recombinase family. However, as a T4 bacteriophage recombinase homologous to RecA/Rad51, UvsX has limited in vitro performance during recombinase polymerase amplification (RPA) due to ATP utilization and DNA affinity.
Lin Zhang +6 more
openaire +2 more sources
Homologous recombination is a vital biological process for DNA repair, genomic stability, and genetic diversity, driven by the RecA/Rad51 recombinase family. However, as a T4 bacteriophage recombinase homologous to RecA/Rad51, UvsX has limited in vitro performance during recombinase polymerase amplification (RPA) due to ATP utilization and DNA affinity.
Lin Zhang +6 more
openaire +2 more sources
[Recombinase Polymerase Amplification and its Applications in Parasite Detection].
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 2016Recombinase polymerase amplification (RPA) is a recently -developed isothermal nucleic-acid-amplification technology that is based on the nucleic acid replication mechanism in T4 bacteriophage. With this technique, nucleic-acid templates can be amplified to measurable levels within 20 min at 37-42 °C. The.
Wen-bin, ZHENG +4 more
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Overview and Future Perspectives of Microfluidic Digital Recombinase Polymerase Amplification (dRPA)
Critical Reviews in Analytical Chemistry, 2022exaly
Recombinase polymerase amplification assays for the identification of pork and horsemeat
Food Chemistry, 2020Olaf Piepenburg, Ahmed Abd El Wahed
exaly
Label-free recombinase polymerase amplification with hyperspectral digital optofluidics
Optics in Health Care and Biomedical Optics XIII, 2023Tianqi Zhou +7 more
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