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Current Protocols in Molecular Biology, 2006
AbstractThe study of protein en masse, or functional proteomics, depends on the availability of full‐length cDNAs in appropriate expression‐ready plasmid vectors for protein expression and functional analysis. Recombinational cloning is a universal cloning technique based on site‐specific recombination that is independent of the insert DNA sequence to ...
Jaehong, Park, Joshua, Labaer
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AbstractThe study of protein en masse, or functional proteomics, depends on the availability of full‐length cDNAs in appropriate expression‐ready plasmid vectors for protein expression and functional analysis. Recombinational cloning is a universal cloning technique based on site‐specific recombination that is independent of the insert DNA sequence to ...
Jaehong, Park, Joshua, Labaer
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2008
Although substantial advances have been made in mycobacterial genetics over the past 15 yr, manipulation of mycobacterial genomes and Mycobacterium tuberculosis in particular, continues to be hindered by problems of relatively poor DNA uptake, slow growth rate, and high levels of illegitimate recombination.
Julia C, van Kessel, Graham F, Hatfull
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Although substantial advances have been made in mycobacterial genetics over the past 15 yr, manipulation of mycobacterial genomes and Mycobacterium tuberculosis in particular, continues to be hindered by problems of relatively poor DNA uptake, slow growth rate, and high levels of illegitimate recombination.
Julia C, van Kessel, Graham F, Hatfull
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2013
Homologous recombination is a complex process that has several important roles: repair of broken DNA, repair of disintegrated replication forks and generation of genetic diversity. Two independent recombination pathways, RecBCD and RecF(OR), operate in Escherichia coli. Both pathways are involved in the initial stages of recombination i.e.
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Homologous recombination is a complex process that has several important roles: repair of broken DNA, repair of disintegrated replication forks and generation of genetic diversity. Two independent recombination pathways, RecBCD and RecF(OR), operate in Escherichia coli. Both pathways are involved in the initial stages of recombination i.e.
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Clinical Reviews in Allergy & Immunology, 2001
In the near future, it can be anticipated that recombinant allergens will be used extensively for research purposes, including structural analysis of allergens, determination of T-cell function, and in animal and human experiments on allergen-induced inflammatory reactions.
C, Grégoire, M D, Chapman
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In the near future, it can be anticipated that recombinant allergens will be used extensively for research purposes, including structural analysis of allergens, determination of T-cell function, and in animal and human experiments on allergen-induced inflammatory reactions.
C, Grégoire, M D, Chapman
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Pediatrics In Review, 1991
Cloning and expression of the human gene encoding erythropoietin has resulted in the availability of recombinant erythropoietin for clinical and laboratory investigation. Results of such investigations are clarifying the mechanisms that regulate production of erythropoietin in health and disease.
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Cloning and expression of the human gene encoding erythropoietin has resulted in the availability of recombinant erythropoietin for clinical and laboratory investigation. Results of such investigations are clarifying the mechanisms that regulate production of erythropoietin in health and disease.
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Immunology Today, 1992
The ability of lymphocyte receptor V, D and J gene segments to rearrange generates much of the receptor diversity that is the hallmark of the immune system. Naturally, the mechanisms of immunoglobulin and T-cell receptor gene recombination are of enormous interest.
F W, Alt +5 more
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The ability of lymphocyte receptor V, D and J gene segments to rearrange generates much of the receptor diversity that is the hallmark of the immune system. Naturally, the mechanisms of immunoglobulin and T-cell receptor gene recombination are of enormous interest.
F W, Alt +5 more
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Russian Journal of Bioorganic Chemistry, 2007
An artificial gene encoding oxyntomodulin was obtained using chemical and enzymatic methods and cloned into Escherichia coli. A recombinant plasmid was constructed containing a hybrid oxyntomodulin gene and Ssp dnaB intein from Synechocystis sp. The expression of the resulting hybrid gene in E.
V N, Stepannenko +4 more
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An artificial gene encoding oxyntomodulin was obtained using chemical and enzymatic methods and cloned into Escherichia coli. A recombinant plasmid was constructed containing a hybrid oxyntomodulin gene and Ssp dnaB intein from Synechocystis sp. The expression of the resulting hybrid gene in E.
V N, Stepannenko +4 more
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