Results 111 to 120 of about 1,248,050 (270)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Development Of A Collaborative Recommendation System Based on Singular Value Decomposition (SVD) on E-Commerce Data

open access: yesJournal of Applied Informatics and Computing
Recommendation systems (RS) are vital tools for mitigating information overload and data sparsity challenges in modern e-commerce platforms. This study focuses on developing and evaluating a Collaborative Filtering (CF) model utilizing Singular Value ...
Galih Mahalisa, Silvia Ratna, M. Muflih
doaj   +1 more source

Content Recommendation Systems in Web-Based Mental Health Care: Real-world Application and Formative Evaluation. [PDF]

open access: yesJMIR Form Res, 2023
Chaturvedi A   +11 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Online Resource-Aware Video Content Recommendation in Edge-Caches for Mobile Users

open access: yesIEEE Access
The coupling of content caching at the wireless network edge and video streaming recommendation systems has been thoroughly investigated to enhance the cache hit and improve the user quality of experience (QoE).
Ana Claudia Bastos Loureiro Moncao   +3 more
doaj   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

Design and Implementation of a Tourism Experimentation Platform for Context-Aware and Sustainable Recommendations

open access: yesApplied Sciences
The digitization of tourism has made numerous platforms available, but there remains a significant shortage of tools capable of promoting local events and activities.
Alessandro Abluton   +6 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

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