Results 41 to 50 of about 183,834 (247)

Genetic attenuation of ALDH1A1 increases metastatic potential and aggressiveness in colorectal cancer

Molecular Oncology, EarlyView.
Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell marker in several malignancies. We established a novel epithelial cell line from rectal adenocarcinoma with unique overexpression of this enzyme. Genetic attenuation of ALDH1A1 led to increased invasive capacity and metastatic potential, the inhibition of proliferation activity, and ultimately ...
Martina Poturnajova, Zuzana Kozovska, Ondrej Pos, Kristina Pavlov, Sachin Gulati, Peter Makovicky, Kristina Jakic, Monika Burikova, Eva Sedlackova, Barbora Svitkova, Silvia Tyciakova, Vojtech Bystry, Nicolas Blavet, Boris Tichy, Matej Hrnciar, Jaroslav Budis, Miroslav Tomas, Peter Dubovan, Georgina Kolnikova, Veronika Repaska, Nikoleta Mojzesova, Eva Zomborska, Daniel Pindak, Michal Mego, Tomas Szemes, Miroslava Matuskova   +25 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Effects of nozzle orientation and spacing on flow characteristics of twin rectangular jets

Nihon Kikai Gakkai ronbunshu, 2023
The effect of the nozzle orientation and the nozzle spacing on the flow characteristics of twin rectangular jets were studied experimentally. The nozzle spacing ratio S/de of a rectangular nozzle with an aspect ratio of 2 was varied from 2.75 to 6.875 ...
Koichi HAYASHIDA, Takahiro KIWATA
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Free Vibration Analysis Of Isotropic Rectangular Plates On Winkler Foundation Using Differential Transform Method

International Journal of Applied Mechanics and Engineering, 2013
A differential transform method (DTM) is used to analyze free transverse vibrations of isotropic rectangular plates resting on a Winkler foundation. Two opposite edges of the plates are assumed to be simply supported.
Y. Kumar
doaj   +1 more source

Numerical Study on Deformation and Failure Characteristics of Rectangular Roadway

Geofluids, 2023
In the practical processes of coal mines and tunnel, rectangular roadways are widely used in the underground environment. However, the stress distribution law of the rock mass around the rectangular roadways is relatively complicated that is influenced ...
Xuejia Li, Baoyang Wu, Yanzhao Zhu, Junting Guo, Zhaolong Li   +4 more
doaj   +1 more source

Rectangular R-Transform as the Limit of Rectangular Spherical Integrals [PDF]

Journal of Theoretical Probability, 2011
In this paper, we connect rectangular free probability theory and spherical integrals. In this way, we prove the analogue, for rectangular or square non-Hermitian matrices, of a result that Guionnet and Maida proved for Hermitian matrices in 2005. More specifically, we study the limit, as $n,m$ tend to infinity, of the logarithm (divided by $n$) of the
openaire   +2 more sources

Evidence of Iron Accumulation in Cerebral Adrenoleukodystrophy: A Potential Novel Disease Mechanism

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT In this first application of Quantitative Susceptibility Mapping Source Separation to cerebral adrenoleukodystrophy, we uncovered alterations in iron and myelin within lesions and normal appearing white matter. As validation, we demonstrate abnormal iron accumulation in those same compartments within primary brain tissue.
Christina L. Nemeth, Mert Sisman, Jinwei Zhang, Hyeong‐geol Shin, Xu Li, Bela Turk, Ali Fatemi, Thanh Nguyen, Eric Mallack   +8 more
wiley   +1 more source

PENGEMBANGAN LKS BERCIRIKAN GUIDED DISCOVERY LEARNING PADA MATERI SEGI EMPAT UNTUK SISWA KELAS VII MTS

Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan, 2016
The objective of the study is to the produce students’ worksheet using the Guided Discovery Learning it is valid, practical and effective. This is a research and developmental design which adopted the model of Plomp.
Ismatul Maula, Subanji Subanji, Sudirman Sudirman   +2 more
doaj   +1 more source