Results 71 to 80 of about 871 (166)
, 2016 The strain used is JC10289 (AB1157 ΔrecA) engineered with a deletion of the araBAD operon as described in Materials and Methods. Plasmids were constructed to express both the indicated RecA (from the tac promoter, uninduced) and the indicated RecX (from ...
Michael M. Cox (64) +5 more
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Fluorescence microscopy of B. subtilis rec+ and ΔrecX cells expressing CFP-RecA.
, 2012 (A) CFP-RecA in exponentially growing cells. (B–E) Cells at 90 (B), 120 (C), 180 (D) and 210 min (E) after induction of DSBs by 0.15 µM MMC. CFP-RecA threads are visible on the nucleoid (some cells whose outline is unclear are denoted by a dotted line ...
Miriam Kaufenstein (109025) +7 more
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The effect of RecX proteins on ATPase activity of RecA variant proteins.
, 2015 (A) Effects of EcRecX protein. Reactions contained 3 μM RecA variant, 5 μM M13mp18 ssDNA, 0.5 μM SSB, and 3 mM ATP. The ATP hydrolysis by RecA protein was initiated by the addition of ATP and SSB protein. After 7 min, 100 nM of RecX protein was added and
Sindhu Chitteni-Pattu (750595) +5 more
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RecX adopts a tandem repeats of three-helix bundle: insights into RecX inhibition of RecA activities [PDF]
Acta Crystallographica Section A Foundations of Crystallography, 2008 C.-Y. Yang +3 more
openaire +1 more source
Bacillus subtilis RecA accessory proteins at the stage of homology search during natural transformation [PDF]
, 2020 Tesis Doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 29-11-2018Esta tesis tiene embargado el acceso al texto completo hasta el 29-05-2018La transformación natural ...
Serrano Álvarez, Ester
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B. subtilis RecA protein accumulation upon SOS induction in different genetic backgrounds.
, 2012 Cells were grown to OD560 = 0.4 in LB medium and exposed to increasing concentrations of MMC for 30 min. The cells were lysed and equivalent protein amounts subjected to 10% SDS-PAGE, followed by immunoblot transfer (see Materials and Methods). (A) rec+,
Miriam Kaufenstein (109025) +7 more
core +1 more source
Dynamic B. subtilis RecA assembly into SsbA-coated ssDNA.
, 2012 It is proposed that RecO (perhaps in concert with RecR) mediates RecA nucleation, and RecF and RecX modulate RecA filament extension. SsbA binds to ssDNA and limits RecA nucleation (step a). SsbA recruits RecO (RecOREco) onto SsbA-coated ssDNA.
Miriam Kaufenstein (109025) +7 more
core +1 more source
, 2018 Double strand breaks (DSBs) in E. coli chromosome (such as those induced by gamma rays) are repaired by recombination repair, during which a certain amount of DNA gets degraded.
Feliciello I
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Herschel-PACS observations of discs in the η Chamaeleontis association [PDF]
, 2015 Context. Protoplanetary discs are the birthplace of planets. Studying protoplanetary discs is the key to constraining theories of planet formation. By observing dust and gas in associations at different ages we can study the evolution of these discs ...
Kamp, I. +18 more
core +2 more sources
, 2020 Additional file 7 Figure S7. Expression of the target genes of ncRv11706A. (A) Expression of few of the target genes of ncRv11706A which are differentially expressed at persistence day-0 compared to mid-exponential growth phase and various time points of
Rami Balasubramanian (8466450) +2 more
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