Results 31 to 40 of about 1,871,408 (339)

Automated and closed clinical-grade manufacturing protocol produces potent NK cells against neuroblastoma cells and AML blasts

Scientific Reports
Natural killer (NK) cells are a promising allogeneic immunotherapy option due to their natural ability to kill tumor cells, and due to their apparent safety.
Farhana Jahan, Leena Penna, Annu Luostarinen, Laurens Veltman, Heidi Hongisto, Kaarina Lähteenmäki, Sabine Müller, Seppo Ylä-Herttuala, Matti Korhonen, Kim Vettenranta, Anita Laitinen, Urpu Salmenniemi, Erja Kerkelä   +12 more
doaj   +1 more source

Modifications in FLAP's second cytosolic loop influence 5‐LOX interaction, inhibitor binding, and leukotriene formation

FEBS Letters, EarlyView.
The enzyme 5‐lipoxygenase (5‐LOX) catalyzes the first step in the biosynthesis of leukotrienes (LTs) involved in inflammatory pathophysiology. After cellular stimulation, 5‐LOX translocates to the nucleus, interacting with the 5‐LOX‐activating protein (FLAP) to form LTA4 from arachidonic acid (AA).
Erik Romp, Katharina Rataj, Stefanie König, Marcia E. Newcomer, Oliver Werz, Ulrike Garscha   +5 more
wiley   +1 more source

Hyperoxia in extreme hemodilution [PDF]

, 2002
Intraoperative surgical blood loss is initially replaced by infusion of red cell-free, cristalloidal or colloidal solutions. When normovolemia is maintained the ensuing dilutional anemia is compensated by an increase of cardiac output and of arterial ...
Habler, Oliver, Kemming, Gregor, Kleen, Martin, Zwissler, Bernhard   +3 more
core   +1 more source

B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses

FEBS Letters, EarlyView.
B cells sense external mechanical forces and convert them into biochemical signals through mechanotransduction. Understanding how malignant B cells respond to physical stimuli represents a groundbreaking area of research. This review examines the key mechano‐related molecules and pathways in B lymphocytes, highlights the most relevant techniques to ...
Marta Sampietro, Marco Cellani, Cristina Scielzo   +2 more
wiley   +1 more source

Standing-wave-excited multiplanar fluorescence in a laser scanning microscope reveals 3D information on red blood cells [PDF]

, 2014
Standing-wave excitation of fluorescence is highly desirable in optical microscopy because it improves the axial resolution. We demonstrate here that multiplanar excitation of fluorescence by a standing wave can be produced in a single-spot laser ...
Amor, Rumelo, Amos, William Bradshaw, Mahajan, Sumeet, McConnell, Gail   +3 more
core   +2 more sources

Systematic literature review evaluating evidence and mechanisms of action for platelet-rich plasma as an antibacterial agent

Journal of Cardiothoracic Surgery, 2021
Platelet rich plasma or PRP is a supraphysiologic concentrate of platelets derived by centrifugation and separation of whole blood components. Along with platelets and plasma, PRP contains various cell types including white blood cells (WBC)/leukocytes ...
Dalip Sethi, Kimberly E. Martin, Sangeeta Shrotriya, Bethany L. Brown   +3 more
doaj   +1 more source

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source

Polymerized bovine hemoglobin solution as a replacement for allogeneic red blood cell transfusion after cardiac surgery: Results of a randomized, double-blind trial [PDF]

, 2002
Background: Blood loss leading to reduced oxygen-carrying capacity is usually treated with red blood cell transfusions. This study examined the hypothesis that a hemoglobin-based oxygen-carrying solution can serve as an initial alternative to red blood ...
Cohen, Marc, Comunale, Mark E., Doblar, Dennis, Goodnough, Lawrence T., Gratz, Irwin, Greilich, Philip E., Jahr, Jonathan S., Levy, Jerrold H., Parr, Grant V.S., Silvay, George, Stewart, Robert W., Vlahakes, Gus J., Wahr, Joyce, Williams, John   +13 more
core   +1 more source

Establishing a GMP-compliant manufacturing process and phase-appropriate analytics for early development of a FiCAR T-cell product with a novel CAR spacer

Scientific Reports
There is a growing demand for chimeric antigen receptor (CAR) -T cells for clinical trials. Consequently, new centers capable of manufacturing advanced therapy medicinal products (ATMPs) are needed.
Annu Luostarinen, Arja Vuorela, Erja Kerkelä, Mimmi Patrikoski, Annika Kotovuori, Jan Koski, Jonna Ahoniemi, Kaarina Lähteenmäki, Jenni Lehtisalo, Terhi Oja, Henrik Paavilainen, Anu Autio, Marie Nyman, Veera Nikoskelainen, Virginie Kergourlay, Endrit Elbasani, Bert van Veen, Anil Thotakura, Hector Monzo, Päivi M. Ojala, Matti Korhonen, Heidi Hongisto, Anita Laitinen   +22 more
doaj   +1 more source

SF3B1 deficiency impairs human erythropoiesis via activation of p53 pathway: implications for understanding of ineffective erythropoiesis in MDS

Journal of Hematology & Oncology, 2018
Background SF3B1 is a core component of splicing machinery. Mutations in SF3B1 are frequently found in myelodysplastic syndromes (MDS), particularly in patients with refractory anemia with ringed sideroblasts (RARS), characterized by isolated anemia ...
Yumin Huang, John Hale, Yaomei Wang, Wei Li, Shijie Zhang, Jieying Zhang, Huizhi Zhao, Xinhua Guo, Jing Liu, Hongxia Yan, Karina Yazdanbakhsh, Gang Huang, Christopher D. Hillyer, Narla Mohandas, Lixiang Chen, Ling Sun, Xiuli An   +16 more
doaj   +1 more source