Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Simulación de una red de acelerómetros para el monitoreo del estado de la malla vial en un sector de la ciudad de Bogotá, un enfoque de aplicación de ITS e IOT [PDF]
Trabajo de InvestigaciónBasados en la investigación de los enfoques de IoT e ITS y todos sus componentes, se propuso una simulación de un escenario, para monitorear el estado de la malla vial de un sector de Bogotá mediante una red de acelerómetros, para
Ramos-Arévalo, Pedro Felipe +1 more
core
The Cu(OTf)2 catalysed microwave assisted synthesis of a new scaffold, 7-aryl-7,8-dihydropyrido[4,3-c]pyridazin-5(6H)-one [PDF]
The synthesis of novel 7-aryl-7,8-dihydropyrido[4,3-c]pyridazin-5(6H)-ones is described including a one-step Mannich-type reaction followed by intramolecular ring closure of ethyl 3-methylpyridazine-4- carboxylate and aldimines, catalysed by the Lewis ...
De Coen, Laurens +5 more
core +2 more sources
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Clasificación de la fracción fina de materiales para base granular provenientes de fuentes de material del municipio de Cundinamarca, a partir de su valor de azul de metileno y equivalente de arena. [PDF]
Trabajo de investigaciónEn el campo de la ingeniería de pavimentos, los agregados pétreos constituyen un pilar importante en la estructura de un proyecto vial.
Olivos-Velásquez, Mayra Alejandra +1 more
core
New views on the emission and structure of the solar transition region
The Sun is the only star that we can spatially resolve and it can be regarded as a fundamental plasma laboratory of astrophysics. The solar transition region (TR), the layer between the solar chromosphere and corona, plays an important role in solar wind
Aiouaz +192 more
core +1 more source
The Structure and Dynamics of the Upper Chromosphere and Lower Transition Region as Revealed by the Subarcsecond VAULT Observations [PDF]
The Very high Angular resolution ULtraviolet Telescope (VAULT) is a sounding rocket payload built to study the crucial interface between the solar chromosphere and the corona by observing the strongest line in the solar spectrum, the Ly-a line at 1216 ...
A. Vourlidas +61 more
core +2 more sources
ErB4 and NdB4 nanostructured powders are produced by mechanochemical synthesis. 5 h mechanical alloying and 4 M HCl acid leaching are used in the production. ErB4 and NdB4 powders exhibit maximum magnetization of 0.4726 emu g−1 accompanied with an antiferromagnetic‐to‐paramagnetic phase transition at about TN = 18 K and 0.132 emu g−1 with a maximum at ...
Burçak Boztemur +5 more
wiley +1 more source
En este ensayo se enfoca la evidencia documental y arqueológica de la comunicación andino-mesoamericana, a través de la navegación oceánica en el Pacifico ecuatorial, que en la conquista española será la principal vía de ingreso al imperio de los incas ...
Gerardo Castro Espinoza
doaj +1 more source
Dynamic Precipitation during High‐Pressure Torsion of a Magnesium–Manganese Alloy
An ultrafine‐grained alloy is produced by high‐pressure torsion of solutionized Mg–1.35 wt% Mn. Precipitation of nanometer‐scale Mn particles during deformation provides pinning sites. This prevents the formation of a bimodal grain structure and results in a finer grain size than for pure Mg.
Julian M. Rosalie, Anton Hohenwarter
wiley +1 more source

