Directed evolution of enzymes at the crossroads of tradition and innovation
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Research on the visual search behavior and decision-making ability of basketball referees. [PDF]
Wang R, Chen L, Wu Y, Zhang Q.
europepmc +1 more source
Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay +2 more
wiley +1 more source
Checking the rules: Comparing bodychecking, head contact, and injury risk across adolescent girls', boys', and para ice sports in Canada. [PDF]
Heming EE +4 more
europepmc +1 more source
Small RNA pathways in mammalian oocytes
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley +1 more source
The Field of Gender Through Metaphors: The Dilemma of Female and Male Referees in the Minds of Football Fans. [PDF]
Engin SG.
europepmc +1 more source
Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard +12 more
wiley +1 more source
Mental health and officiating performance. [PDF]
Taylor N, Hancock D, Sullivan P.
europepmc +1 more source
Physical Demands of Handball Referees: A Big Data Analysis between the EHF Men's and Women's EUROs 2022. [PDF]
Lozano D +4 more
europepmc +1 more source
The influence of social forces: evidence from the behavior of football referees. [PDF]
Dohmen, Thomas
core

