Results 21 to 30 of about 4,074 (239)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

High-quality development path for methanol and reconfiguration of its storage and transportation system driven by the “dual carbon” goals

You-qi chuyun
ObjectiveAmid the “carbon peaking and carbon neutrality” strategy and energy structure transformation, China faces challenges such as new energy power consumption hurdles and low utilization rates of traditional oil and gas pipelines.
Shangxin ZHAO
doaj   +1 more source

Cross-country pipeline inspection data analysis and testing of probabilistic degradation models

Journal of Pipeline Science and Engineering, 2021
Pipelines are the most efficient and safest means for the transportation of oil, gas, and refined petroleum products. Potentially severe consequences of pipeline failures make reliability and risk assessment an essential aspect of safe operation. However,
Faisal Khan, Rioshar Yarveisy, Rouzbeh Abbassi   +2 more
doaj   +1 more source

Karl Popper and the Mechanisms of Hydrogen Embrittlement

Advanced Engineering Materials, EarlyView.
Representation of the beginning of loss of ductility rather than embrittlement. Small concentrations of hydrogen in a diffusible form within iron are well‐established to harm the mechanical integrity of steels. There are theories that attempt to explain the pernicious role of hydrogen.
H. K. D. H. Bhadeshia
wiley   +1 more source

OntOMat: Toward Ontology‐Based Product and Process Design Engineering and Optimization Solutions Fueling Circular Value Chains

Advanced Engineering Materials, EarlyView.
The OntOMat ontology establishes a structured framework for polymer matrix fiber reinforced composite materials, integrating manufacturing processes, characterization methods, and multiscale design through the VDI/VDE 3682 formalized process description standard.
Nicolas Christ, Jörg Hohe, Martin Kördel, Michael Giese, Niklas Toffert, Christian Seidel, Olaf Rehme, Patrik Schneider, Oliver Focke, Elie Abi Aoun, Claas Falldorf, Beñat Gutierrez, Alexandra Kühn, Sven Torstrick-von der Lieth, Christoph Herden, Erik Johannsen, Jan‐Uwe R. Schmidt, Felix Winkelmann, Andreas Schott, Daniel Schott   +19 more
wiley   +1 more source

Integration of OpenCV‐Based Microscopic Adhesive Volume Measurement Into a Pyiron Workflow for Automated Data Analysis

Advanced Engineering Materials, EarlyView.
Residual adhesive after electrode loading in adhesive‐assisted resistance spot welding is quantified through a traceable experimental‐to‐digital workflow. Chromatic confocal topography provides calibrated surface‐height data, while OpenCV detects the electrode imprint and integrates adhesive height into comparable volume metrics.
Sung‐Min Wi, Jiangdong Zhao
wiley   +1 more source

Advances in Sustainable and Wearable Textile Based Soft Robotics

Advanced Functional Materials, EarlyView.
This Review examines advances in wearable textile‐based soft robotics, focusing on sustainable materials, integrated sensing, and scalable actuation. It discusses manufacturing and system integration across healthcare, assistive robotics, prosthetics, and human–machine interfaces, and highlights key challenges in circular design, including life‐cycle ...
Zahir Abbas, Marzia Dulal, Sophia K. Novoselov, Victoria K. Novoselov, Kostya S. Novoselov, Shaila Afroj, Nazmul Karim   +6 more
wiley   +1 more source

Economic evaluation and optimization of repurposing in-service refined oil pipelines for gaseous CO2 transport

You-qi chuyun
ObjectiveRepurposing in-service refined oil pipelines for gaseous CO2 transport is a highly efficient strategy for large-scale CO2 delivery. However, its economic viability depends on variables such as repurposing schemes and carbon subsidies, yet a ...
Jianfeng MAN, Xin OUYANG, Yiting DONG, Weibin WANG, Chong CHAI, Wenlong JIA, Sheng SUN, Zichao HU   +7 more
doaj   +1 more source

Stability of Medicinal Nanoemulsions to Spaceflight: Insights From the StarMed Experiment

Advanced Healthcare Materials, EarlyView.
Chitosan‐coated melatonin nanoemulsions (MN) recovered from a sounding rocket mission preserve droplet size and controlled‐release behavior, whereas uncoated nanoemulsions show ageing‐driven droplet growth and increased accessible drug fraction. Simple polymer coatings thus enhance the flight resilience of liquid space medicines under cumulative launch
Modupe Adebowale, Nguyen Van Duc Long, Philip M. Williams, Jens Hauslage, Volker Hessel   +4 more
wiley   +1 more source