Results 271 to 280 of about 9,098,225 (351)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Climate-driven fuel-cost-based assessment of raw and torrefied biomass for greenhouse heating across different climatic regions of Korea. [PDF]

Sci Rep
Park S, Oh KC, Kim D.
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

The impact of annual price revision based on external reference pricing on medicines between 2012 and 2023. [PDF]

Health Econ Rev
Mendes JZ, Teixeira I, Guerreiro JP, Dimitrovová K, Leal FS, Rodrigues AT.   +5 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Temporal trends and regional disparities in costs and affordability of non-insulin antidiabetic drugs in China, 2015-2022: a retrospective observational study. [PDF]

BMJ Open
Liu X, Lei F, Shukar S, Liu X, Wei H, Chen C, Fang Y, Yang C.   +7 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Integrating Yield Stability and Gross Revenue: Multi-Year Evaluation of <i>Coffea canephora</i> Genotypes. [PDF]

Plants (Basel)
Kolln AM, Almeida RN, Rocha RB, Partelli FL, Teixeira AL, Araújo LFB, Espindula MC.   +6 more
europepmc   +1 more source

Hypothetical food purchase tasks and eating behavior: A systematized review and future directions. [PDF]

J Exp Anal Behav
Rodriguez Perez M, Hone LSE, Almog S, McVay MA, Dallery J, Berry MS.   +5 more
europepmc   +1 more source