FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Mine ventilation system reliability evaluation based on a Markov chain. [PDF]
Sci Rep, 2022 Liu L, Liu J, Zhou Q.
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Clinical Validation of Novel Digital Measures: Statistical Methods for Reliability Evaluation. [PDF]
Digit Biomark, 2023 Ratitch B +5 more
europepmc +1 more source
FEBS Open Bio, EarlyView.14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa +2 more
wiley +1 more source
Research on three-state reliability evaluation method of high reliability system based on multi-source prior information. [PDF]
PeerJ Comput Sci, 2023 Huang J, Huang Z, Zhan X.
europepmc +1 more source
Digital twins to accelerate target identification and drug development for immune‐mediated disorders
FEBS Open Bio, EarlyView.Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley +1 more source
EMUs Apron Board Structural Design Based on Weld Fatigue Life Analysis
机车电传动, 2015 This article took CJ-2 EMUs apron board as a research example, and adopted FE-safe verity module equivalent effective stress method to analyze weld fatigue based on main S-N curve. The analyzed and optimized result will be a reference for the reliability
WANG Guangming +4 more
doaj
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Cryogenic Reliability Evaluation of Glass Fabric-Reinforced Composites Using Novel Slip-Prevention Method. [PDF]
Materials (Basel), 2022 Jeong YC +4 more
europepmc +1 more source