Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Using life-history traits to explain bird population responses to changing weather variability [PDF]
Bird population dynamics are expected to change in response to increased weather variability, an expression of climate change. The extent to which species are sensitive to effects of weather on survival and reproduction depends on their life-history ...
Braak, C.J.F., ter +4 more
core +2 more sources
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu +10 more
wiley +1 more source
Trace minerals profile of blood serum and estrual mucus in repeat breeder Kankrej cows
Aim: The study was carried out with an idea to ascertain involvement of trace minerals in failure of conception in Knakrej cow. Materials and Methods: The animals under study were comprised of 10 normal (Group-I) and 20 repeat breeder (Group-II) Kankrej ...
N F Chaudhari +5 more
doaj +1 more source
Differential coupling of gibberellin responses by Rht-B1c suppressor alleles and Rht-B1b in wheat highlights a unique role for the DELLA N-terminus in dormancy [PDF]
During the Green Revolution, substantial increases in wheat (Triticum aestivum) yields were realized, at least in part, through the introduction of the Reduced height (Rht)-B1b and Rht-D1b semi-dwarfing alleles.
Chandler, Peter Michael +3 more
core +1 more source
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao +3 more
wiley +1 more source
Perbedaan Viskositas Mucous Serviks dan Tipologi Ferning pada Sapi Aceh Repeat Breeding dan Fertil
ABSTRAK. Efisiensi reproduksi ternak sangat ditentukan berdasarkan deteksi estrus. Terdapat beberapa indikator estrus yang dapat dijadikan patokan deteksi estrus diantaranya keluarnya mucous serviks. Mucous serviks akan diamati sifat fisiknya diantaranya
Cut Nila Thasmi +7 more
doaj +1 more source
Cisgenesis, a new tool for traditional plant breeding, should be exempted from the regulation on genetically modified organisms in a step by step approach [PDF]
Modern potato breeding requires over 100,000 seedlings per new variety. Main reasons are (1) the increasing number of traits that have to be combined in this tetraploid vegetatively propagated crop, and (2) an increasing number of traits (e.g ...
Jacobsen, E., Schouten, H.J.
core +2 more sources
Repeated superovulation treatments in Kivircik ewes during breeding and nonbreeding seasons
The present study compared superovulation and embryo recovery rates applied repeatedly during breeding and nonbreeding seasons in the same 20 Kivircik sheep. Trials 1 and 3 were performed during the breeding season and the trial 2 was performed in the nonbreeding season.
Ustuner, Burcu +7 more
openaire +3 more sources
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source

