Results 141 to 150 of about 3,681,265 (301)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Strengthening Rural Health With N-of-1 Trials and Single-Case Experimental Designs. [PDF]

Aust J Rural Health
Nikles J, McDonald S.
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Sample size determination across study designs in health professions education research: a methodology guide. [PDF]

F1000Res
Naqvi WM, Divvi A, Kengadaran S, Pashine AA, Baladaniya M, Patel A, Kandakurthi PK, Muthukrishnan R.   +7 more
europepmc   +1 more source

Optimal discrimination designs [PDF]

We consider the problem of constructing optimal designs for model discrimination between competing regression models. Various new properties of optimal designs with respect to the popular T-optimality criterion are derived, which in many circumstances ...
Dette, Holger, Titoff, Stefanie
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Evaluating Traditional Bonesetting Interventions: A Methodological Review and Framework for Clinical Research. [PDF]

Cureus
Hua JA, Shan BH, Na T, Mu WD, Qi DY, Damba TA, Zagdsuren B, Ganbat D.   +7 more
europepmc   +1 more source

Outperforming completely randomized designs. [PDF]

Bi-randomization designs have become increasingly popular in industry because some of the factors under investigation are often hard-to-change. It is well-known that the resulting compound symmetric error structure not only affects estimation and ...
Goos, Peter, Vandebroek, Martina
core  

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Reliability of Difference Scores Obtained From Nested Data Within a Multivariate Generalizability Theory Framework. [PDF]

Educ Psychol Meas
Karatoprak Ersen R, Lee WC, Yarbrough DB.   +2 more
europepmc   +1 more source