Results 101 to 110 of about 35,153 (171)

Development of a loop‐mediated isothermal amplification‐lateral flow dipstick (LAMP‐LFD) assay and on‐site rapid detection evaluation for Rodentibacter heylii and Rodentibacter pneumotropicus

Animal Models and Experimental Medicine, EarlyView.
This study established a closed‐tube LAMP‐LFD assay for detecting Rodentibacter heylii and Rodentibacter pneumotropicus. The method exhibited high sensitivity (detection limits of 10−5 ng/μL for R. heylii and 10−4 ng/μL for R. pneumotropicus), high specificity, reliable performance with clinical samples, and effective validation in experimental ...
Huiqiong Yan, Sisi Chen, Yuhan Gan, Jing Xing, Yufang Feng, Xin Wen, Jie Fang, Jiangtao Du, Shasang Zhou, Honggang Guo, Xiaoyin Jin, Zhiyuan Wang, Junhao Tao, Lingqun Lu, Qingming Kong, Huazhong Ying, Wei Han, Fangwei Dai   +17 more
wiley   +1 more source

Programmable molecular microscopy: CRISPR/Cas fluorescent probes revolutionizing spatiotemporal genomic imaging. [PDF]

Theranostics
Zhong XY, Yang YX, Xiong YF, Ye GC, Gong X, Zhong ML, He HD, Wang SG, Xia QD.   +8 more
europepmc   +1 more source

Therapeutic Applications of Stimuli‐Based Release and Engineering of Extracellular Vesicles

Advanced NanoBiomed Research, EarlyView.
This review summarizes the effects of endogenous and exogenous stimuli, their effects on the natural release of extracellular vesicles, as well as their uptake and release. It also gives an overview of stimuli‐responsive EVs and their therapeutic applications. Extracellular vesicles (EVs), nano‐ to microsized lipid bilayer membrane‐bound particles, are
Gloria Kemunto, Kristen Dellinger
wiley   +1 more source

Development of Recombinase Polymerase Amplification and CRISPR-Cas12a-Enhanced Isothermal Amplification Assays for Strongyloides stercoralis DNA Detection: A Pilot Study. [PDF]

Am J Trop Med Hyg
Lontuo-Fogang R, Bennuru S, Nutman TB.
europepmc   +1 more source

Tensile Stimulation in Biorelevant Culture Conditions Enhances MSC and TPSC Tenogenesis on Aligned Electrospun Scaffolds

Advanced NanoBiomed Research, EarlyView.
Biomimetic electrospun scaffold incorporating GDF‐7‐loaded mesoporous silica nanoparticles, combined with mechanical stimulation and physiological oxygen tension, guides tenogenic differentiation of mesenchymal stromal cell and tendon progenitor stem cell. This integrated approach enhances cell proliferation, matrix deposition, and tendon‐specific gene
Vera Citro, Aldo R. Boccaccini, Nicholas R. Forsyth, Tina P. Dale   +3 more
wiley   +1 more source

Assembly of Protein‐DNA Framework Nanostructures: Structurally Defining Protein‐DNA Interfaces With Aptamer

Angewandte Chemie, EarlyView.
Rationally designed DNA multivalent aptamers direct specific protein binding to drive self‐assembly into protein‐DNA frameworks (PDFs). Structurally defined protein‐aptamer interfaces organize proteins into triangles, linear and ladderlike chains, and periodic two‐dimensional arrays, enabling precise control over orientation, connectivity, and overall ...
Zhe Zhang, Xuanyu Nan, Zhengyu Huang, Jin Jin, Cheng Tian, Lian Chen, Xiaoli Hu, Huawei He, Yuhe Renee Yang, Cheng Zhi Huang, Chengde Mao, Hua Zuo   +11 more
wiley   +2 more sources

Revealing the inherent design principles of the genetic code via an error correcting code representation. [PDF]

Sci Rep
Aharon A, Polak P, Yaari G.
europepmc   +1 more source

Nonviral Gold Nanoparticle‐Mediated Delivery of CRISPR‐Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells

Advanced NanoBiomed Research, EarlyView.
CRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair   +8 more
wiley   +1 more source

Unambiguous chromosome identification by multicolour fluorescence in situ hybridisation reveals the genome organisation and evolution of sheepgrass (Leymus chinensis). [PDF]

BMC Plant Biol
Sun M, Hu S, Li T, Lv N, Han J, Li Y, Lu J, Wang Z, He T, Sun M, Qi Z, Xiong Z, Wu L.   +12 more
europepmc   +1 more source

Rewriting Tumor Immunity With Bacterial Membrane Vesicles: A Versatile and Potent Therapeutic Strategy

Advanced NanoBiomed Research, EarlyView.
Bacterial membrane vesicles (BMVs) emerge as promising platforms for cancer immunotherapy owing to their intrinsic adjuvant properties and tunable cargo delivery capabilities. Their ability to modulate the tumor microenvironment, enhance antitumor immune responses, and support personalized therapeutic strategies highlights their growing potential as ...
Md Sifat Rahi, Md Mijanur Rahman, Asma Talukder, Urooj Yousaf Virk, Jennifer C. Wilson, Nigel McMillan, Ming Q. Wei   +6 more
wiley   +1 more source