Results 91 to 100 of about 1,062,480 (303)

ATP-dependent structural change of the eukaryotic clamp-loader protein, replication factor C [PDF]

Proceedings of the National Academy of Sciences, 2000
The eukaryotic DNA sliding clamp that keeps DNA polymerase engaged at a replication fork, called proliferating cell nuclear antigen (PCNA), is loaded onto the 3′ ends of primer DNA through its interaction with a heteropentameric protein complex called replication factor C (RFC). The ATPase activity of RFC is necessary for formation of a functional
Y, Shiomi, J, Usukura, Y, Masamura, K, Takeyasu, Y, Nakayama, C, Obuse, H, Yoshikawa, T, Tsurimoto   +7 more
openaire   +2 more sources

The non-canonical role of Aurora-A in DNA replication

Frontiers in Oncology, 2015
Aurora-A is a well-known mitotic kinase that regulates mitotic entry, spindle formation, and chromosome maturation as a canonical role. During mitosis, Aurora-A protein is stabilized by its phosphorylation at Ser51 via blocking anaphase promoting complex/
Takaaki eTsunematsu, Rieko eArakaki, Akiko eYamada, Naozumi eIshimaru, Yasusei eKudo   +4 more
doaj   +1 more source

A Role of hIPI3 in DNA Replication Licensing in Human Cells. [PDF]

PLoS ONE, 2016
The yeast Ipi3p is required for DNA replication and cell viability in Sacharomyces cerevisiae. It is an essential component of the Rix1 complex (Rix1p/Ipi2p-Ipi1p-Ipi3p) that is required for the processing of 35S pre-rRNA in pre-60S ribosomal particles ...
Yining Huang, Aftab Amin, Yan Qin, Ziyi Wang, Huadong Jiang, Lu Liang, Linjing Shi, Chun Liang   +7 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Disruption of hepatitis C virus RNA replication through inhibition of host protein geranylgeranylation [PDF]

Proceedings of the National Academy of Sciences, 2003
Hepatitis C virus (HCV) RNA replication depends on viral protein association with intracellular membranes, but the influence of membrane composition on viral replication is unclear. We report that HCV RNA replication and assembly of the viral replication complex require geranylgeranylation of one or more host proteins.
Jin, Ye, Chunfu, Wang, Rhea, Sumpter, Michael S, Brown, Joseph L, Goldstein, Michael, Gale   +5 more
openaire   +2 more sources

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

The C, V and W proteins of Nipah virus inhibit minigenome replication

Journal of General Virology, 2008
Nipah virus (NiV) is a recently emergent, highly pathogenic, zoonotic paramyxovirus of the genus Henipavirus. Like the phosphoprotein (P) gene of other paramyxoviruses, the P gene of NiV is predicted to encode three additional proteins, C, V and W.
Katrina, Sleeman, Bettina, Bankamp, Kimberly B, Hummel, Michael K, Lo, William J, Bellini, Paul A, Rota   +5 more
openaire   +2 more sources

Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein.

PLoS Pathogens, 2013
The nonstructural protein NS5A has emerged as a new drug target in antiviral therapies for Hepatitis C Virus (HCV) infection. NS5A is critically involved in viral RNA replication that takes place at newly formed membranes within the endoplasmic reticulum
Dorothee A Vogt, Grégory Camus, Eva Herker, Brian R Webster, Chia-Lin Tsou, Warner C Greene, Tien-Sze Benedict Yen, Melanie Ott   +7 more
doaj   +1 more source

Inhibition of cellular protein secretion by norwalk virus nonstructural protein p22 requires a mimic of an endoplasmic reticulum export signal. [PDF]

, 2013
Protein trafficking between the endoplasmic reticulum (ER) and Golgi apparatus is central to cellular homeostasis. ER export signals are utilized by a subset of proteins to rapidly exit the ER by direct uptake into COPII vesicles for transport to the ...
Crawford, Sue E., Estes, M. K., Guix Arnau, Susana, Katayama, K., Sharp, T. M.   +4 more
core   +2 more sources

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source