The Eukaryotic Mismatch Recognition Complexes Track with the Replisome during DNA Synthesis.
PLoS Genetics, 2015 During replication, mismatch repair proteins recognize and repair mispaired bases that escape the proofreading activity of DNA polymerase. In this work, we tested the model that the eukaryotic mismatch recognition complex tracks with the advancing ...
Joanna E Haye, Alison E Gammie
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Mcm10 promotes rapid isomerization of CMG-DNA for replisome bypass of lagging strand DNA blocks
eLife, 2017 Replicative helicases in all cell types are hexameric rings that unwind DNA by steric exclusion in which the helicase encircles the tracking strand only and excludes the other strand from the ring.
Lance D Langston +7 more
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Where and When Bacterial Chromosome Replication Starts: A Single Cell Perspective
Frontiers in Microbiology, 2018 Bacterial chromosomes have a single, unique replication origin (named oriC), from which DNA synthesis starts. This study describes methods of visualizing oriC regions and the chromosome replication in single living bacterial cells in real-time.
Damian Trojanowski +2 more
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Single-molecule visualization of fast polymerase turnover in the bacterial replisome
eLife, 2017 The Escherichia coli DNA replication machinery has been used as a road map to uncover design rules that enable DNA duplication with high efficiency and fidelity.
Jacob S Lewis +10 more
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Bacteriophage T7 DNA polymerase – sequenase [PDF]
, 2014 An ideal DNA polymerase for chain-terminating DNA sequencing should possess the following features: (1) incorporate dideoxy- and other modified nucleotides at an efficiency similar to that of the cognate deoxynucleotides; (2) high processivity; (3) high ...
Zhu, Bin
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Atomistic Molecular Dynamics Simulations of Mitochondrial DNA Polymerase gamma : Novel Mechanisms of Function and Pathogenesis [PDF]
, 2017 DNA polymerase gamma (Pol gamma) is a key component of the mitochondrial DNA replisome and an important cause of neurological diseases. Despite the availability of its crystal structures, the molecular mechanism of DNA replication, the switch between ...
Euro, Liliya +5 more
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MCM2–7-dependent cohesin loading during S phase promotes sister-chromatid cohesion
eLife, 2018 DNA replication transforms cohesin rings dynamically associated with chromatin into the cohesive form to establish sister-chromatid cohesion. Here, we show that, in human cells, cohesin loading onto chromosomes during early S phase requires the ...
Ge Zheng +3 more
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Geminivirus replication protein decreases PCNA sumoylation at two acceptor sites [PDF]
, 2019 Geminiviruses are plant viruses with circular, single-stranded DNA (ssDNA) genomes that infect a broad range of plants causing substantial crop diseases worldwide.
Arroyo-Mateos, Manuel +9 more
core
Chromosome segregation drives division site selection in Streptococcus pneumoniae. [PDF]
, 2017 Accurate spatial and temporal positioning of the tubulin-like protein FtsZ is key for proper bacterial cell division. <i>Streptococcus pneumoniae</i> (pneumococcus) is an oval-shaped, symmetrically dividing opportunistic human pathogen ...
Kjos, M. +2 more
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Replisome Architecture and Dynamics in Escherichia coli [PDF]
Journal of Biological Chemistry, 2006 The field of DNA replication was launched upon discovery of the first DNA polymerase by Kornberg and Lehman (1, 2). DNA polymerase I displayed the novel and highly exciting property of template-directed enzymatic action, and like a split personality, DNApolymerase I could also degradeDNA, from either direction.
openaire +2 more sources