Results 11 to 20 of about 955,297 (305)

A live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells

open access: yesNature Communications, 2020
Small extracellular vesicles called exosomes affect multiple autocrine and paracrine cellular phenotypes. Understanding the function of exosomes requires a variety of tools, including live imaging.
B. Sung   +8 more
semanticscholar   +1 more source

Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus

open access: yesBMC Developmental Biology, 2001
BackgroundSeveral Cre reporter strains of mice have been described, in which a lacZ gene is turned on in cells expressing Cre recombinase, as well as their daughter cells, following Cre-mediated excision of a loxP-flanked transcriptional "stop" sequence.
S. Srinivas   +6 more
semanticscholar   +1 more source

Comparative Analysis of NanoLuc Luciferase and Alkaline Phosphatase Luminescence Reporter Systems for Phage-Based Detection of Bacteria

open access: yesBioengineering, 2022
Reporter phage assays are a promising alternative to culture-based assays for rapidly detecting viable bacteria. The reporter systems used in phage-based detection are typically enzymes and their corresponding substrates that provide a signal following ...
Shalini Wijeratne   +2 more
doaj   +1 more source

Ziołowe terapie ojca Klimuszki

open access: yesHerbalism, 2015
Franciszkanin o. Czesław Andrzej Klimuszko (1905–1980) wyróżnia się wśród innych polskich autorytetów w dziedzinie ziołolecznictwa. Był fenomenem, jakiego nie da się porównać z żadnym lekarzem naturalistą.
Krzysztof Kamiński
doaj   +1 more source

Characterization of the human GnRH neuron developmental transcriptome using a GNRH1-TdTomato reporter line in human pluripotent stem cells

open access: yesDisease Models & Mechanisms, 2020
Gonadotropin-releasing hormone (GnRH) neurons provide a fundamental signal for the onset of puberty and subsequent reproductive functions by secretion of gonadotropin-releasing hormone.
Carina Lund   +10 more
doaj   +1 more source

PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells

open access: yeseLife, 2022
Prime editing is a recently developed CRISPR/Cas9 based gene engineering tool that allows the introduction of short insertions, deletions, and substitutions into the genome. However, the efficiency of prime editing, which typically achieves editing rates
Dorottya Anna Simon   +6 more
doaj   +1 more source

A systematic evaluation of the design and context dependencies of massively parallel reporter assays

open access: yesNature Methods, 2020
Massively parallel reporter assays (MPRAs) functionally screen thousands of sequences for regulatory activity in parallel. To date, there are limited studies that systematically compare differences in MPRA design.
Jason C. Klein   +7 more
semanticscholar   +1 more source

MAVS-Based Reporter Systems for Real-Time Imaging of EV71 Infection and Antiviral Testing

open access: yesViruses, 2023
Enterovirus consists of a variety of viruses that could cause a wide range of illness in human. The pathogenesis of these viruses remains incompletely understood and no specific treatment is available.
Xiaozhen Li   +7 more
doaj   +1 more source

Analysis of media reports to understand tobacco industry tactics in undermining tobacco tax policies in Bangladesh

open access: yesTobacco Induced Diseases, 2018
Background Mass media plays an important role in influencing tobacco control policies. The tobacco industry also uses the mass media to promote their products and undermine tobacco control policies, in particular tobacco tax policies.
Sushanta Kumar Singha
doaj   +1 more source

The activation of the Sox2 RR2 pluripotency transcriptional reporter in human breast cancer cell lines is dynamic and labels cells with higher tumorigenic potential

open access: yesFrontiers in Oncology, 2014
The striking similarity displayed at the mechanistic level between tumorigenesis and the generation of induced pluripotent stem cells and the fact that genes and pathways relevant for embryonic development are reactivated during tumor progression ...
Juan Manuel eIglesias   +20 more
doaj   +1 more source

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