Results 1 to 10 of about 417,548 (214)

Luciferase‐Induced Photouncaging: Bioluminolysis

Angewandte Chemie - International Edition, 2019
Abstract Bioluminescence resonance energy transfer (BRET) has been widely used for studying dynamic processes in biological systems such as protein–protein interactions and other signaling events. Aside from acting as a reporter, BRET can also turn on functions in living systems.
Dalu Chang, Eric Lindberg, Suihan Feng
exaly   +4 more sources

Monitoring SARS-CoV-2 Infection Using a Double Reporter-Expressing Virus

Microbiology Spectrum, 2022
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the highly contagious agent responsible for the coronavirus disease 2019 (COVID-19) pandemic.
Kevin Chiem, Jun-Gyu Park, Desarey Morales Vasquez, Richard K. Plemper, Jordi B. Torrelles, James J. Kobie, Mark R. Walter, Chengjin Ye, Luis Martinez-Sobrido   +8 more
doaj   +1 more source

Dopamine-induced arrestin recruitment and desensitization of the dopamine D4 receptor is regulated by G protein-coupled receptor kinase-2

Frontiers in Pharmacology, 2023
The dopamine D4 receptor (D4R) is expressed in the retina, prefrontal cortex, and autonomic nervous system and has been implicated in attention deficit hyperactivity disorder (ADHD), substance use disorders, and erectile dysfunction.
Viktor Burström, Richard Ågren, Nibal Betari, Marta Valle-León, Marta Valle-León, Emilio Garro-Martínez, Francisco Ciruela, Francisco Ciruela, Kristoffer Sahlholm, Kristoffer Sahlholm, Kristoffer Sahlholm, Kristoffer Sahlholm   +11 more
doaj   +1 more source

Comparative Analysis of NanoLuc Luciferase and Alkaline Phosphatase Luminescence Reporter Systems for Phage-Based Detection of Bacteria

Bioengineering, 2022
Reporter phage assays are a promising alternative to culture-based assays for rapidly detecting viable bacteria. The reporter systems used in phage-based detection are typically enzymes and their corresponding substrates that provide a signal following ...
Shalini Wijeratne, Arindam Bakshi, Joey Talbert   +2 more
doaj   +1 more source

A luminescence-based method to assess antigen presentation and antigen-specific T cell responses for in vitro screening of immunomodulatory checkpoints and therapeutics

Frontiers in Immunology, 2023
Investigations into the strength of antigen-specific responses in vitro is becoming increasingly relevant for decision making in early-phase research of novel immunotherapeutic approaches, including adoptive cell but also immune checkpoint inhibitor (ICI)
Jimena Álvarez Freile, Yuzhu Qi, Lisa Jacob, Maria Franceskin Lobo, Harm Jan Lourens, Gerwin Huls, Edwin Bremer   +6 more
doaj   +1 more source

Aberrant Polycystin-1 Expression Results in Modification of Activator Protein-1 Activity, whereas Wnt Signaling Remains Unaffected [PDF]

, 2004
Polycystin-1, the polycystic kidney disease 1 gene product, has been implicated in several signaling complexes that are known to regulate essential cellular functions. We investigated the role of polycystin-1 in Wnt signaling and activator protein-1 (
Arnould, Bhunia, Duyndam, Giles, Grotewold, Huan, Kim, Kimberling, Kugoh, Loghman-Adham, Löhning, Morin, Moser, Muto, Newby, Ouwens, Parnell, Peters, Pritchard, Rivat, Rodova, Saadi-Kheddouci, Scheffers, Shaulian, Smits, Sorenson, Spaargaren, Streets, Sutters, Trudel, Upadhya, van Adelsberg, van Dam, van Dam, van Dam, van Dam, van de Wetering, van de Wetering   +37 more
core   +7 more sources

Reverse Genetic Approaches for the Generation of Full Length and Subgenomic Replicon of EV71 Virus

Frontiers in Microbiology, 2021
Enterovirus 71 (EV71) is a neurotropic pathogen that causes hand, foot, and mouth disease (HFMD) and it has been consistently associated with severe neurological, cardiac, and respiratory complications.
Hang Yang, Xiaohui Zhao, Meng Xun, Chaofeng Ma, Hongliang Wang, Hongliang Wang   +5 more
doaj   +1 more source

Luciferase‐free Luciferin Electrochemiluminescence

Angewandte Chemie International Edition, 2022
AbstractLuciferin is one of Nature's most widespread luminophores, and enzymes that catalyze luciferin luminescence are the basis of successful commercial “glow” assays for gene expression and metabolic ATP formation. Herein we report an electrochemical method to promote firefly's luciferin luminescence in the absence of its natural biocatalyst ...
Mattia Belotti, Mohsen M. T. El‐Tahawy, Li‐Juan Yu, Isabella C. Russell, Nadim Darwish, Michelle L. Coote, Marco Garavelli, Simone Ciampi   +7 more
openaire   +4 more sources

Luciferase Controlled Protein Interactions

Journal of the American Chemical Society, 2021
Protein trafficking and protein-protein interactions (PPI) are central to regulatory processes in cells. Induced dimerization systems have been developed to control PPI and regulate protein trafficking (localization) or interactions. Chemically induced dimerization (CID) has proven to be a robust approach to control protein interactions and ...
Dalu Chang, Suihan Feng, Vladimir Girik, Howard Riezman, Nicolas Winssinger   +4 more
openaire   +3 more sources

Using Biosensors to Study Protein–Protein Interaction in the Hippo Pathway

Frontiers in Cell and Developmental Biology, 2021
The Hippo signaling network is dependent on protein–protein interactions (PPIs) as a mechanism of signal transduction to regulate organ size, cellular proliferation and differentiation, tumorigenesis, and other cellular processes.
Alexander Pipchuk, Xiaolong Yang
doaj   +1 more source