Results 31 to 40 of about 325,683 (268)

Luciferase Assay

, 2021
Luciferase Assay NRE ...
Björn Schnute (11715137)
core   +1 more source

Bioluminescent Enzyme Inhibition Based Assay of Metal Nanoparticles [PDF]

Журнал Сибирского федерального университета: Серия Биология, 2017
The bioluminescent enzymatic bioassays for assessment of nanomaterial biotoxicity using the soluble or immobilized coupled enzyme system of luminous bacteria NAD(P)Н:FMN-oxidoreductase + luciferase (Red + Luc) as a test system were employed in this study.
Yana V. Fritsler, Elena N. Esimbekova, Valentina A. Kratasyuk   +2 more
doaj  

Improvements in Smartphone and Night Vision Imaging Technologies Enable Low Cost, On-Site Assays of Bioluminescent Cells

Frontiers in Bioengineering and Biotechnology, 2021
Technologies enabling on-site environmental detection or medical diagnostics in resource-limited settings have a strong disruptive potential compared to current analytical approaches that require trained personnel in laboratories with immobile, resource ...
Mark Wienhold, Andrew Kirkpatrick, Tingting Xu, Steven Ripp, Steven Ripp, Gary Sayler, Dan Close   +6 more
doaj   +1 more source

Luciferase-based reporter assay as described in Fig 1 at different scales.

, 2021
(PDF)
Zhenzhong Yu (5704235), Reinhard Fischer (182192), Christian Streng (11596833), Julian Ingelfinger (11596845), Kai Leister (11596842), Ramon F. Seibeld (11596836), Olumuyiwa A. Igbalajobi (11596839)   +6 more
core   +1 more source

A Nitrogen Molecular Sensing System, Comprised of the ALLANTOINASE and UREIDE PERMEASE 1 Genes, Can Be Used to Monitor N Status in Rice

Frontiers in Plant Science, 2018
Nitrogen (N) is an essential nutrient for plant growth and development, but its concentration in the soil is often insufficient for optimal crop production.
Dong-Keun Lee, Mark C. F. R. Redillas, Harin Jung, Seowon Choi, Youn Shic Kim, Ju-Kon Kim   +5 more
doaj   +1 more source

Screening of Fungi for Antimycobacterial Activity Using a Medium-Throughput Bioluminescence-Based Assay

Frontiers in Microbiology, 2021
There is a real and urgent need for new antibiotics able to kill Mycobacteria, acid-fast bacilli capable of causing multiple deadly diseases. These include members of the Mycobacterium tuberculosis complex, which causes the lung disease tuberculosis (TB)
Alexander B. J. Grey, Melissa M. Cadelis, Melissa M. Cadelis, Yiwei Diao, Duckchul Park, Thomas Lumley, Bevan S. Weir, Brent R. Copp, Siouxsie Wiles   +8 more
doaj   +1 more source

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

FEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source

The distribution of ATP within tomato (Lycopersicon esculentum Mill.) embryos correlates with germination whee as total ATP concentration does not

, 2002
The distribution of ATP in tomato seeds was visualized by monitoring the luminescence of frozen sections on top of a gel containing all the components of the luciferase reaction, but excluding ATP.
Joosen, R.V.L., Hilhorst, H.W.M., Spoelstra, P.   +2 more
core   +1 more source

Multiplex Hextuple Luciferase Assaying

, 2022
We recently expanded the commonly used dual luciferase assaying method toward multiplex hextuple luciferase assaying, allowing monitoring the activity of five experimental pathways against one control at the same time.
Young, Damian W, Gonzalez, Yezabel, Venken, Koen J T, Chang, Lyra, Gallego-Flores, Tatiana, Sarrion-Perdigones, Alejandro   +5 more
core   +1 more source

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

Molecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, Michael Chopp, Zheng Gang Zhang, Yi Zhang   +12 more
wiley   +1 more source