Results 11 to 20 of about 417,647 (313)

A High-Content Screen for the Identification of Plant Extracts with Insulin Secretion-Modulating Activity

open access: yesPharmaceuticals, 2021
Bioactive plant compounds and extracts are of special interest for the development of pharmaceuticals. Here, we describe the screening of more than 1100 aqueous plant extracts and synthetic reference compounds for their ability to stimulate or inhibit ...
Roland Hager   +10 more
doaj   +1 more source

Streamlined and Robust Stage-Specific Profiling of Gametocytocidal Compounds Against Plasmodium falciparum

open access: yesFrontiers in Cellular and Infection Microbiology, 2022
Malaria elimination is dependent on the ability to target both the pathogenic and transmissible stages of the human malaria parasite, Plasmodium falciparum.
Janette Reader   +3 more
doaj   +1 more source

Triggering cell death in cancers using self-illuminating nanocomposites

open access: yesFrontiers in Chemistry, 2022
Bioinspired photocatalysis has resulted in efficient solutions for many areas of science and technology spanning from solar cells to medicine. Here we show a new bioinspired semiconductor nanocomposite (nanoTiO2-DOPA-luciferase, TiDoL) capable of ...
Tijana Rajh   +6 more
doaj   +1 more source

Synthesis of IFN-β by Virus-Infected Chicken Embryo Cells Demonstrated with Specific Antisera and a New Bioassay [PDF]

open access: yes, 2004
Transcripts of interferon-α(IFN-α) and IFN-β genes are present in virus-infected chicken cells, but because of a lack of appropriate assays and reagents, it was unclear if biologically active IFN-β is secreted.
Harlin, Olof   +4 more
core   +1 more source

Luciferase-LOV BRET enables versatile and specific transcriptional readout of cellular protein-protein interactions

open access: yeseLife, 2019
Technologies that convert transient protein-protein interactions (PPIs) into stable expression of a reporter gene are useful for genetic selections, high-throughput screening, and multiplexing with omics technologies. We previously reported SPARK (Kim et
Christina K Kim   +3 more
doaj   +1 more source

Bioluminescence-Driven Optogenetics

open access: yesLife, 2020
Bioluminescence-based technologies are among the most commonly used methods to quantify and visualise physiology at the cellular and organismal levels.
Macià Sureda-Vives, Karen S. Sarkisyan
doaj   +1 more source

The Inducible lac Operator-Repressor System Is Functional in Zebrafish Cells

open access: yesFrontiers in Genetics, 2021
BackgroundZebrafish are a foundational model organism for studying the spatio-temporal activity of genes and their regulatory sequences. A variety of approaches are currently available for editing genes and modifying gene expression in zebrafish ...
Sierra S. Nishizaki   +8 more
doaj   +1 more source

Quantitative immunohistochemistry using an antibody-fused bioluminescent protein

open access: yesBioTechniques, 2020
We established a quantitative detection method for immunohistochemistry based on a reference standard light-emitting diode, protein microarray and antibody-fused bioluminescent protein.
Ke-Yong Wang   +6 more
doaj   +1 more source

Evidence for the role of proteoglycans in cation-mediated gene transfer [PDF]

open access: yes, 1996
We report evidence that gene complexes, consisting of polycations and plasmid DNA enter cells via binding to membrane-associated proteoglycans. Treatment of HeLa cells with sodium chlorate, a potent inhibitor of proteoglycan sulfation, reduced luciferase
Baldeschwieler, John D.   +1 more
core   +1 more source

Construction and application of luminescent strain of Pectobacterium carotovorum harboring luxCDABE operon

open access: yes浙江大学学报. 农业与生命科学版, 2021
Pectobacterium carotovorum is one of the top ten plant bacterial pathogens in the world. In order to facilitate the observation of the pathogen infection process at the early stage and in vivo quantification of bacterial growth without the tissue ...
ZHONG Lingkun   +4 more
doaj   +1 more source

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