Results 11 to 20 of about 325,683 (268)

Luciferase-LOV BRET enables versatile and specific transcriptional readout of cellular protein-protein interactions

open access: yeseLife, 2019
Technologies that convert transient protein-protein interactions (PPIs) into stable expression of a reporter gene are useful for genetic selections, high-throughput screening, and multiplexing with omics technologies. We previously reported SPARK (Kim et
Christina K Kim   +3 more
doaj   +1 more source

Bioluminescence-Driven Optogenetics

open access: yesLife, 2020
Bioluminescence-based technologies are among the most commonly used methods to quantify and visualise physiology at the cellular and organismal levels.
Macià Sureda-Vives, Karen S. Sarkisyan
doaj   +1 more source

The Inducible lac Operator-Repressor System Is Functional in Zebrafish Cells

open access: yesFrontiers in Genetics, 2021
BackgroundZebrafish are a foundational model organism for studying the spatio-temporal activity of genes and their regulatory sequences. A variety of approaches are currently available for editing genes and modifying gene expression in zebrafish ...
Sierra S. Nishizaki   +8 more
doaj   +1 more source

An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum [PDF]

open access: yes, 2012
This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum.
Hasenkamp Sandra   +14 more
core   +1 more source

Quantitative immunohistochemistry using an antibody-fused bioluminescent protein

open access: yesBioTechniques, 2020
We established a quantitative detection method for immunohistochemistry based on a reference standard light-emitting diode, protein microarray and antibody-fused bioluminescent protein.
Ke-Yong Wang   +6 more
doaj   +1 more source

Optimisation of bioluminescent reporters for use with mycobacteria [PDF]

open access: yes, 2010
Mycobacterium tuberculosis, the causative agent of tuberculosis, still represents a major public health threat in many countries. Bioluminescence, the production of light by luciferase-catalyzed reactions, is a versatile reporter technology with multiple
Jorge Ripoll   +36 more
core   +1 more source

Construction and application of luminescent strain of Pectobacterium carotovorum harboring luxCDABE operon

open access: yes浙江大学学报. 农业与生命科学版, 2021
Pectobacterium carotovorum is one of the top ten plant bacterial pathogens in the world. In order to facilitate the observation of the pathogen infection process at the early stage and in vivo quantification of bacterial growth without the tissue ...
ZHONG Lingkun   +4 more
doaj   +1 more source

nmarzano/Luciferase-refolding-smFRET_manuscript: nmarzano/Luciferase-refolding-smFRET_manuscript

open access: yes, 2022
Code to analyse and plot single-molecule FRET data contained within the manuscript entitled 'Real-time single-molecule observation of chaperone-assisted protein folding'
nmarzano
core   +1 more source

Effect of Prooxidative Natural Products: Comparison of the OSI1 (YKL071w) Promoter Luciferase Construct from Yeast with an Nrf2/Keap Reporter System

open access: yesApplied Sciences, 2020
The oxidative stress response (OSR) in yeast is under the control of oxidation-sensitive cysteines in the Yap1p transcription factor, and fusion of the Yap1p-dependent OS-induced promoter of the YKL071w gene (OSI1) to a luciferase coding sequence makes a
Ivan Schlembach   +5 more
doaj   +1 more source

The Protein Environment Restricts the Intramolecular Charge Transfer Character of the Luciferine/Luciferase Complex

open access: yes, 2023
The elecronic characterization of the luciferine/luciferase complex is fundamental to tune its photophysical properties and develop more efficient devices based on this luminiscent system.
Henar, Mateo-delaFuente   +3 more
core   +1 more source

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