Luciferase-free Luciferin Electrochemiluminescence
Angewandte Chemie International Edition, 2022 Luciferin is one of Nature's most widespread luminophores, and enzymes that catalyze luciferin luminescence are the basis of successful commercial “glow” assays for gene expression and metabolic ATP formation.
Simone Ciampi (23286055) +7 more
core +5 more sources
The C-terminal truncated splicing variant of NK1R negatively modulates substance P-stimulated NK1R signaling. [PDF]
FEBS Open BioThe neurokinin 1 receptor exists as full‐length (NK1L) and C‐terminally truncated (NK1S) splice variants. We show that NK1S heterodimerizes with NK1L, impairing Gαq coupling and Ca2+ mobilization while enhancing β‐arrestin1 recruitment. NK1S suppresses substance P‐driven gene expression and cell migration, revealing NK1S as an endogenous biased ...
Nguyen LP +8 more
europepmc +2 more sources
Identification of (20R)-protopanaxadiol from Panax ginseng as a novel anti-SARS-CoV-2 compound. [PDF]
FEBS Open BioWe established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Takeda M +4 more
europepmc +2 more sources
Luciferase-based reporter assay.
, 2021 (A) Luciferase was cloned behind the conJ promoter and transformed into wildtype, the ΔfphA- or the ΔlreA-deletion strains. After 16 h incubation in the dark, luminescence was measured every 10 min for 90 min under the indicated light conditions.
Zhenzhong Yu (5704235) +6 more
core +1 more source
The Protein Environment Restricts the Intramolecular Charge Transfer Character of the Luciferine/Luciferase Complex [PDF]
, 2023 The elecronic characterization of the luciferine/luciferase complex is fundamental to tune its photophysical properties and develop more efficient devices based on this luminiscent system.
Henar, Mateo-delaFuente +3 more
core +1 more source
Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay [PDF]
, 2008 To study the coordination of different lipid synthesis pathways during membrane biogenesis it is useful to work with an experimental system where membrane biogenesis occurs rapidly and in an inducible manner.
Nohturfft, A, Zhang, S
core +1 more source
An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum [PDF]
, 2012 This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum.
Hasenkamp Sandra +15 more
core +1 more source
Bioluminescence-Driven Optogenetics
Life, 2020 Bioluminescence-based technologies are among the most commonly used methods to quantify and visualise physiology at the cellular and organismal levels.
Macià Sureda-Vives, Karen S. Sarkisyan
doaj +1 more source
The Inducible lac Operator-Repressor System Is Functional in Zebrafish Cells
Frontiers in Genetics, 2021 BackgroundZebrafish are a foundational model organism for studying the spatio-temporal activity of genes and their regulatory sequences. A variety of approaches are currently available for editing genes and modifying gene expression in zebrafish ...
Sierra S. Nishizaki +8 more
doaj +1 more source
Quantitative immunohistochemistry using an antibody-fused bioluminescent protein
BioTechniques, 2020 We established a quantitative detection method for immunohistochemistry based on a reference standard light-emitting diode, protein microarray and antibody-fused bioluminescent protein.
Ke-Yong Wang +6 more
doaj +1 more source