Results 21 to 30 of about 341,486 (310)

Optimisation of bioluminescent reporters for use with mycobacteria [PDF]

open access: yes, 2010
Mycobacterium tuberculosis, the causative agent of tuberculosis, still represents a major public health threat in many countries. Bioluminescence, the production of light by luciferase-catalyzed reactions, is a versatile reporter technology with multiple
Jorge Ripoll   +36 more
core   +1 more source

Construction and application of luminescent strain of Pectobacterium carotovorum harboring luxCDABE operon

open access: yes浙江大学学报. 农业与生命科学版, 2021
Pectobacterium carotovorum is one of the top ten plant bacterial pathogens in the world. In order to facilitate the observation of the pathogen infection process at the early stage and in vivo quantification of bacterial growth without the tissue ...
ZHONG Lingkun   +4 more
doaj   +1 more source

The distribution of ATP within tomato (Lycopersicon esculentum Mill.) embryos correlates with germination whee as total ATP concentration does not [PDF]

open access: yes, 2002
The distribution of ATP in tomato seeds was visualized by monitoring the luminescence of frozen sections on top of a gel containing all the components of the luciferase reaction, but excluding ATP.
Joosen, R.V.L.   +2 more
core   +1 more source

Effect of Prooxidative Natural Products: Comparison of the OSI1 (YKL071w) Promoter Luciferase Construct from Yeast with an Nrf2/Keap Reporter System

open access: yesApplied Sciences, 2020
The oxidative stress response (OSR) in yeast is under the control of oxidation-sensitive cysteines in the Yap1p transcription factor, and fusion of the Yap1p-dependent OS-induced promoter of the YKL071w gene (OSI1) to a luciferase coding sequence makes a
Ivan Schlembach   +5 more
doaj   +1 more source

Multiplex Hextuple Luciferase Assaying [PDF]

open access: yes, 2022
We recently expanded the commonly used dual luciferase assaying method toward multiplex hextuple luciferase assaying, allowing monitoring the activity of five experimental pathways against one control at the same time.
Young, Damian W   +5 more
core   +1 more source

nmarzano/Luciferase-refolding-smFRET_manuscript: nmarzano/Luciferase-refolding-smFRET_manuscript

open access: yes, 2022
Code to analyse and plot single-molecule FRET data contained within the manuscript entitled 'Real-time single-molecule observation of chaperone-assisted protein folding'
nmarzano
core   +1 more source

Molecular detection of bioluminescent dinoflagellates in surface waters of the Patagonian Shelf during early austral summer 2008 [PDF]

open access: yes, 2014
We investigated the distribution of bioluminescent dinoflagellates in the Patagonian Shelf region using “universal” PCR primers for the dinoflagellate luciferase gene.
Martha Valiadi (576318)   +24 more
core   +3 more sources

Dual-Luciferase-Based Fast and Sensitive Detection of Malaria Hypnozoites for the Discovery of Antirelapse Compounds

open access: yes, 2020
Efforts to eradicate Plasmodium vivax malaria are hampered by the presence of hypnozoites, persisting stages in the liver that can reactivate after prolonged periods of time enabling further transmission and causing renewed disease.
Anne-Marie Zeeman (276913)   +7 more
core   +1 more source

Detection of Matrix Metalloproteinase Activity by Bioluminescence via Intein-Mediated Biotinylation of Luciferase

open access: yesSensors, 2018
We report bioluminescence analysis of matrix metalloproteinase (MMP) activity in biological substances using a surface-bound luciferase probe. Intein-fused luciferase protein enables site-specific biotinylation of luciferase in the presence of N-terminus
Duc Long Nguyen   +5 more
doaj   +1 more source

A multifunctional, synthetic Gaussia princeps luciferase reporter for live imaging of Candida albicans infections [PDF]

open access: yes, 2009
Peer ...
Rachini, Anna   +12 more
core   +1 more source

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