Results 21 to 30 of about 276,444 (269)

Bioluminescence-Driven Optogenetics

open access: yesLife, 2020
Bioluminescence-based technologies are among the most commonly used methods to quantify and visualise physiology at the cellular and organismal levels.
Macià Sureda-Vives, Karen S. Sarkisyan
doaj   +1 more source

The Inducible lac Operator-Repressor System Is Functional in Zebrafish Cells

open access: yesFrontiers in Genetics, 2021
BackgroundZebrafish are a foundational model organism for studying the spatio-temporal activity of genes and their regulatory sequences. A variety of approaches are currently available for editing genes and modifying gene expression in zebrafish ...
Sierra S. Nishizaki   +8 more
doaj   +1 more source

Quantitative immunohistochemistry using an antibody-fused bioluminescent protein

open access: yesBioTechniques, 2020
We established a quantitative detection method for immunohistochemistry based on a reference standard light-emitting diode, protein microarray and antibody-fused bioluminescent protein.
Ke-Yong Wang   +6 more
doaj   +1 more source

Construction and application of luminescent strain of Pectobacterium carotovorum harboring luxCDABE operon

open access: yes浙江大学学报. 农业与生命科学版, 2021
Pectobacterium carotovorum is one of the top ten plant bacterial pathogens in the world. In order to facilitate the observation of the pathogen infection process at the early stage and in vivo quantification of bacterial growth without the tissue ...
ZHONG Lingkun   +4 more
doaj   +1 more source

Effect of Prooxidative Natural Products: Comparison of the OSI1 (YKL071w) Promoter Luciferase Construct from Yeast with an Nrf2/Keap Reporter System

open access: yesApplied Sciences, 2020
The oxidative stress response (OSR) in yeast is under the control of oxidation-sensitive cysteines in the Yap1p transcription factor, and fusion of the Yap1p-dependent OS-induced promoter of the YKL071w gene (OSI1) to a luciferase coding sequence makes a
Ivan Schlembach   +5 more
doaj   +1 more source

Detection of Matrix Metalloproteinase Activity by Bioluminescence via Intein-Mediated Biotinylation of Luciferase

open access: yesSensors, 2018
We report bioluminescence analysis of matrix metalloproteinase (MMP) activity in biological substances using a surface-bound luciferase probe. Intein-fused luciferase protein enables site-specific biotinylation of luciferase in the presence of N-terminus
Duc Long Nguyen   +5 more
doaj   +1 more source

Chemical composition and biological activities of the Agaricus mushrooms

open access: yesMongolian Journal of Chemistry, 2014
Two species of Agaricus mushroom grown in Mongolia were analyzed for their element content. Biological activity and chemical components study of Agaricus, grown in the Mongolian flora has been investigated for the first time.
L Munkhgerel   +6 more
doaj   +1 more source

IgE-Crosslinking-Induced Luciferase Expression Test as a Sensitive Indicator of Anisakis Allergy

open access: yesAntibodies
Background: Anisakis allergy has been increasing, and the diagnosis of it is based on specific serum IgE detection. Recently, the IgE-crosslinking-induced luciferase expression (EXiLE) test has been proposed as convenient tool for detecting functionally ...
Haruyo Akiyama   +5 more
doaj   +1 more source

Bioluminescent Enzyme Inhibition Based Assay of Metal Nanoparticles [PDF]

open access: yesЖурнал Сибирского федерального университета: Серия Биология, 2017
The bioluminescent enzymatic bioassays for assessment of nanomaterial biotoxicity using the soluble or immobilized coupled enzyme system of luminous bacteria NAD(P)Н:FMN-oxidoreductase + luciferase (Red + Luc) as a test system were employed in this study.
Yana V. Fritsler   +2 more
doaj  

In vitro–In vivo Hybrid Approach for Studying Modulation of NRF2 in Immortalized Bovine Mammary Cells

open access: yesFrontiers in Animal Science, 2021
Nuclear factor erythroid 2-related factor 2 (NRF2) plays a key role in the response to oxidative stress. Diets containing known NRF2 modulators could be used to minimize oxidative stress in dairy cows.
Hunter R. Ford   +2 more
doaj   +1 more source

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