Results 1 to 10 of about 10,758 (111)

Mapping the structural dynamics of red- and blue-emitting beetle luciferases revealed by HDX-MS [PDF]

Life Science Alliance
Using HDX-MS, this study demonstrates that conformational flexibility in beetle luciferases governs bioluminescence color, revealing how active site dynamics and domain-specific mutations fine-tune red- and blue-shifted light emission. Beetle luciferases
Abdul-Rahman Kharbatli, Juliana C Ferreira, Nathan M Lui, Rawdah Karwt, Shaolong Zhu, Liaqat Ali, Wael M Rabeh   +6 more
doaj   +2 more sources

Red-shifted d-luciferin analogues and their bioluminescence characteristics [PDF]

RSC Chemical Biology
d-Luciferin (d-LH2) is the most used substrate for beetle luciferases in various bioluminescence applications. Here, we successfully synthesized six d-LH2 analogues including 5′,7′-dimethoxy-d-LH2 and 7′-methylnaphthol-d-LH2 as novel compounds.
Pratchaya Watthaisong, Chadaporn Kantiwiriyawanitch, Watcharapa Jitkaroon, Aisaraphon Phintha, Ittiphat Klayparn, Narin Lawan, Philaiwarong Kamutira, Daisuke Sasaki, Surawit Visitsatthawong, Somchart Maenpuen, Ruchanok Tinikul, Jeerus Sucharitakul, Ryo Nishihara, Kazuki Niwa, Yoshihiro Nakajima, Yoshihiro Ohmiya, Pimchai Chaiyen   +16 more
doaj   +2 more sources

Selection and Engineering of Novel Brighter Bioluminescent Reporter Gene and Color- Tuning Luciferase for pH-Sensing in Mammalian Cells [PDF]

Biosensors
Firefly luciferases have been extensively used for bioanalytical applications, including their use as bioluminescent reporters, biosensors, and for bioimaging biological and pathological processes.
Vanessa R. Bevilaqua, Gabriel F. Pelentir, Moema A. Hausen, Eliana A. R. Duek, Vadim R. Viviani   +4 more
doaj   +2 more sources

Coelenterazine Analogs for Bioassays and Molecular Imaging [PDF]

Sensors
Coelenterazine (CTZ) is a common substrate of marine luciferases upon emission of bioluminescence (BL) in living organisms. Because CTZ works as a “luminophore” in the process of BL emission, the chemical modification has been centered for improving the ...
Sung-Bae Kim, Genta Kamiya, Tadaomi Furuta, Shojiro A. Maki   +3 more
doaj   +2 more sources

Effects of surfactants on luminescence intensity and duration of the minimal luciferase picALuc [PDF]

Biophysics and Physicobiology
Bioluminescence is an oxidation-mediated chemical reaction involving the luciferase enzyme and the luciferin substrate. To date, various artificial luciferases have been developed, and their luminescence properties have been investigated.
Yuki Ohmuro-Matsuyama, Kento Motoyama, Genta Kamiya, Ryogo Takai, Nobuo Kitada, Mitsuru Hattori, Shojiro A. Maki, Takeharu Nagai, Tadaomi Furuta   +8 more
doaj   +2 more sources

Applications of bioluminescence-mediated photodynamic therapy (BL-PDT) for the treatment of deep-seated tumours [PDF]

Discover Oncology
Bioluminescence, the light generated through biochemical reactions involving luciferases in living organisms, has been the focus of extensive research due to its diverse applications.
Mpho Mohlongo, Blassan P. George, Heidi Abrahamse   +2 more
doaj   +2 more sources

An optimized luciferin formulation for NanoLuc-based in vivo bioluminescence imaging [PDF]

Scientific Reports
Bioluminescence imaging (BLI) is widely used in preclinical biomedical research for noninvasive tracking of cell populations and biochemical events in vivo.
Chao Gao, Yan Wu, Connor Fitzgerald, Hui Wang, Tim Ugo, Tetsuo Uyeda, Wenhui Zhou, Yichi Su, Thomas A. Kirkland, Michael Z. Lin   +9 more
doaj   +2 more sources

Construction of Staphylococcus aureus bioluminescence tracing system based on Antares2

陆军军医大学学报, 2023
Objective To construct a new fusion luciferase-labeled Staphylococcus aureus (S. aureus) tracing system. Methods An S. aureus tracer strain was constructed by using DNA homologous recombination technique.
HU Zhen, SHANG Weilong, RAO Xiancai
doaj   +1 more source

Substrate cooperativity in marine luciferases. [PDF]

PLoS ONE, 2012
Marine luciferases are increasingly used as reporters to study gene regulation. These luciferases have utility in bioluminescent assay development, although little has been reported on their catalytic properties in response to substrate concentration ...
George Tzertzinis, Ezra Schildkraut, Ira Schildkraut   +2 more
doaj   +1 more source

Bioluminescence Color-Tuning Firefly Luciferases: Engineering and Prospects for Real-Time Intracellular pH Imaging and Heavy Metal Biosensing

Biosensors, 2022
Firefly luciferases catalyze the efficient production of yellow-green light under normal physiological conditions, having been extensively used for bioanalytical purposes for over 5 decades.
Vadim R. Viviani, Gabriel F. Pelentir, Vanessa R. Bevilaqua   +2 more
doaj   +1 more source