Results 41 to 50 of about 11,605 (288)

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

, 2020
The functioning of bioluminescent systems in most of the known marine organisms is based on the oxidation reaction of the same substrate—coelenterazine (CTZ), catalyzed by luciferase.
Ludmila A. Frank, Eugenia E. Bashmakova, Vasilisa V. Krasitskaya   +2 more
core   +1 more source

Macromolecular assembly of bioluminescent protein nanoparticles for enhanced imaging

Materials Today Bio, 2022
Bioluminescence imaging has advantages over fluorescence imaging, such as minimal photobleaching and autofluorescence, and greater signal-to-noise ratios in many complex environments.
Enya Li, Caroline K. Brennan, Aaron Ramirez, Jo A. Tucker, Nina Butkovich, Vijaykumar S. Meli, Anastasia A. Ionkina, Edward L. Nelson, Jennifer A. Prescher, Szu-Wen Wang   +9 more
doaj   +1 more source

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

FEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source

Group entropy analysis of luciferases.

, 2018
Group entropy analysis of luciferases.
Danielle Leatherby (5714819), Louis Clark (5714816), John Perozich (5714825), Alexander J. Ropelewski (237444), Elizabeth Krilich (5714822)   +4 more
core   +1 more source

Codon-Optimized Luciferase Variants for Mammalian Gene Expression in Culture and in Vivo

Molecular Imaging, 2012
Luciferases have proven to be useful tools in advancing our understanding of biologic processes. Having a multitude of bioluminescent reporters with different properties is highly desirable.
Casey A. Maguire, Johannes C. van der Mijn, Marja H. Degeling, Danielle Morse, Bakhos A. Tannous   +4 more
doaj   +1 more source

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

Molecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, Michael Chopp, Zheng Gang Zhang, Yi Zhang   +12 more
wiley   +1 more source

Establishment of a humanized patient‐derived xenograft mouse model of high‐grade serous ovarian cancer for preclinical evaluation of combination immunotherapy

Molecular Oncology, EarlyView.
We have established a humanized orthotopic patient‐derived xenograft (Hu‐oPDX) mouse model of high‐grade serous ovarian cancer (HGSOC) that recapitulates human tumor–immune interactions. Using combined anti‐PD‐L1/anti‐CD73 immunotherapy, we demonstrate the model's improved biological relevance and enhanced translational value for preclinical ...
Luka Tandaric, Line Bjørge, Martine Rott Lode, Cecilie Fredvik Torkildsen, Pia Aehnlich, Rammah Elnour, Daniela Elena Costea, Lars Andreas Akslen, Liv Cecilie Vestrheim Thomsen, Emmet McCormack, Katrin Kleinmanns   +10 more
wiley   +1 more source

Comparison of luminescence activity of QL-nanoKAZ with other CTZ-utilizing luciferases using aequorin as a light standard.

, 2022
Comparison of luminescence activity of QL-nanoKAZ with other CTZ-utilizing luciferases using aequorin as a light standard.
Mikako Shirouzu (272392), Yuto Sumida (1696852), Jun-ichi Sato (133357), Shun-ichi Sekine (1372875), Yuiko Sahara-Miura (1696846), Yuri Tomabechi (10644646), Satoshi Inouye (203922), Takamitsu Hosoya (205623)   +7 more
core   +1 more source

Structural Comparison of Firefly Luciferase with the Latent Luciferase CG6178

The FASEB Journal, 2015
Firefly luciferase shares high sequence identity to insect fatty acyl‐CoA synthetases (ACSLs). These homologous enzymes operate through a similar two‐step mechanism. In the first step, the substrate is adenylated to form an AMP ester. Both firefly luciferase and ACSLs can accept fatty acid substrates.
Spencer Adams, Markus Bohn, Celia Schiffer, Stephen Miller   +3 more
openaire   +1 more source

CD47 promotes mitogen‐activated protein kinase and epithelial‐to‐mesenchymal transition molecular programs to drive prometastatic phenotypes in non‐small cell lung cancer

Molecular Oncology, EarlyView.
Beyond its role in immune evasion, this study identified that CD47 drives tumor‐intrinsic signaling in non‐small cell lung cancer (NSCLC). Transcriptomic profiling and functional studies revealed that CD47 regulates cell adhesion, migration, and metastasis through an ERK–EMT signaling axis.
Asa P.Y. Lau, Lilian G. Zhai, Ryunosuke Hoshi, Zackary Rousseau, Abraam Zakhary, Yin Fang Wu, Rola M. Saleeb, Heyu Ni, Kelsie L. Thu   +8 more
wiley   +1 more source