Results 1 to 10 of about 5,703 (128)

Fast kinetics of photoprotein emitting species [PDF]

Scientific Reports
Coelenterazine is the most common substrate for light-emitting reactions identified in luminous marine organisms. Among bioluminescent proteins engaging coelenterazine as a luciferin, Ca2+-regulated photoproteins form stable enzyme-substrate complexes ...
Elena V. Eremeeva, Sergey I. Bartsev, Natalia P. Malikova, Willem J. H. van Berkel, Eugene S. Vysotski   +4 more
doaj   +2 more sources

AB-MDMSBA-Not a Synthetic Cannabinoid Receptor Agonist. [PDF]

Pharmacol Res Perspect
This study uses classical in vitro pharmacology approaches to establish that AB‐MDMSBA does not bind to or activate the cannabinoid CB1 receptor and therefore should not be classified as a synthetic cannabinoid receptor agonist. ABSTRACT Synthetic cannabinoids remain one of the most prominent classes of New Psychoactive Substances (NPS).
Patel M, Ganeshan D, Horgan I, Partington H, Glass M.   +4 more
europepmc   +2 more sources

Availability and occurrence of coelenterazine in a Swedish fjord to maintain Amphiura filiformis bioluminescence [PDF]

Scientific Reports
The bioluminescent European brittle star Amphiura filiformis produces blue light at the arm-spine level thanks to a biochemical reaction involving coelenterazine as substrate and a Renilla-like luciferase as an enzyme.
Constance Coubris, Khaled Mirzaei, Laurent Duchatelet, Jérôme Mallefet   +3 more
doaj   +2 more sources

Coelenterazine Analogs for Bioassays and Molecular Imaging [PDF]

Sensors
Coelenterazine (CTZ) is a common substrate of marine luciferases upon emission of bioluminescence (BL) in living organisms. Because CTZ works as a “luminophore” in the process of BL emission, the chemical modification has been centered for improving the ...
Sung-Bae Kim, Genta Kamiya, Tadaomi Furuta, Shojiro A. Maki   +3 more
doaj   +2 more sources

Design of Ctenophore Ca²⁺-Regulated Photoprotein Berovin Capable of Being Converted into Active Protein Under Physiological Conditions: Computational and Experimental Approaches [PDF]

Life
Here, we describe (1) the AlphaFold-based structural modeling approach to identify amino acids of the photoprotein berovin that are crucial for coelenterazine binding, and (2) the production and characterization of berovin mutants with substitutions of ...
Ludmila P. Burakova, Nikita V. Ivanisenko, Natalia V. Rukosueva, Vladimir A. Ivanisenko, Eugene S. Vysotski   +4 more
doaj   +2 more sources

Improved Messenger RNA Stability and Biocompatibility Through Self-Gelatinizable Nucleic Acids. [PDF]

Biotechnol Bioeng
ABSTRACT Recent advances in the chemical synthesis and modification of messenger RNA (mRNA) have generated growing interest in mRNA‐based therapeutics. However, the inherent instability of mRNA in vivo and during storage remains a major challenge, requiring the development of safe and effective delivery systems.
Tanifuji T, Kusamori K, Tanaka C, Sakai H, Itakura S, Nishikawa M.   +5 more
europepmc   +2 more sources

Advanced Bioluminescence Reporter with Engineered Gaussia Luciferase via Sequence-Guided Mutagenesis [PDF]

Biosensors
Gaussia luciferase (GLuc) is the preeminent secreted luciferase widely used in cell-based reporter assays. By employing sequence-guided mutagenesis informed by alignments of diverse copepod luciferase sequences, we identified key amino acids that ...
Vinayakumar Gedi, Eun Hye Kim, Bohyun Oh, Young-Pil Kim   +3 more
doaj   +2 more sources

Insights into the bioluminescence systems of three sea pens (Cnidaria: Anthozoa): from de novo transcriptome analyses to biochemical assays [PDF]

Open Biology
Bioluminescence is the production of visible light by living organisms. It occurs through the oxidation of luciferin substrates catalysed by luciferase enzymes.
Laurent Duchatelet, Gabriela A. Galeazzo, Constance Coubris, Laure Bridoux, René Rezsohazy, Marcelo R. S. Melo, Martin Marek, Danilo T. Amaral, Sam Dupont, Anderson Garbuglio de Oliveira, Jérôme Delroisse   +10 more
doaj   +2 more sources

The key residue responsible for the red shift of bioluminescence spectra of light-sensitive Ca2+-regulated photoproteins of ctenophores [PDF]

Scientific Reports
Isoforms of some ctenophore photoproteins show different maxima of bioluminescence spectra— the ones of mnemiopsin and bolinopsin have λmax at 490 and 500 nm, while λmax for velamin isoforms are at 500 and 508 nm.
Ludmila P. Burakova, Eugene S. Vysotski
doaj   +2 more sources

A brittle star is born: Ontogeny of luminous capabilities in Amphiura filiformis. [PDF]

PLoS ONE
Bioluminescence is the production of visible light by living organisms thanks to a chemical reaction, implying the oxidation of a substrate called luciferin catalyzed by an enzyme, the luciferase.
Constance Coubris, Laurent Duchatelet, Sam Dupont, Jérôme Mallefet   +3 more
doaj   +2 more sources