Results 1 to 10 of about 6,471 (200)

Availability and occurrence of coelenterazine in a Swedish fjord to maintain Amphiura filiformis bioluminescence [PDF]

Scientific Reports
The bioluminescent European brittle star Amphiura filiformis produces blue light at the arm-spine level thanks to a biochemical reaction involving coelenterazine as substrate and a Renilla-like luciferase as an enzyme.
Constance Coubris, Khaled Mirzaei, Laurent Duchatelet, Jérôme Mallefet   +3 more
doaj   +3 more sources

Coelenterazine Analogs for Bioassays and Molecular Imaging [PDF]

Sensors
Coelenterazine (CTZ) is a common substrate of marine luciferases upon emission of bioluminescence (BL) in living organisms. Because CTZ works as a “luminophore” in the process of BL emission, the chemical modification has been centered for improving the ...
Sung-Bae Kim, Genta Kamiya, Tadaomi Furuta, Shojiro A. Maki   +3 more
doaj   +6 more sources

BRET-Based Approaches for Ion Channels: Emerging Applications and Future Directions. [PDF]

Arch Pharm (Weinheim)
Bioluminescence resonance energy transfer (BRET) is a versatile method for studying ligand–protein interactions. Its compatibility with high‐throughput screening and real‐time kinetics has made it widely used in drug discovery. Here, we summarize recent applications of BRET‐based methods for studying ligand binding to ion channels, highlighting key ...
Kock N, Heitzer L, Massa J, Taterra M, Bermúdez M, Koch O, Schiedel M.   +6 more
europepmc   +2 more sources

Fast kinetics of photoprotein emitting species [PDF]

Scientific Reports
Coelenterazine is the most common substrate for light-emitting reactions identified in luminous marine organisms. Among bioluminescent proteins engaging coelenterazine as a luciferin, Ca2+-regulated photoproteins form stable enzyme-substrate complexes ...
Elena V. Eremeeva, Sergey I. Bartsev, Natalia P. Malikova, Willem J. H. van Berkel, Eugene S. Vysotski   +4 more
doaj   +2 more sources

AB-MDMSBA-Not a Synthetic Cannabinoid Receptor Agonist. [PDF]

Pharmacol Res Perspect
This study uses classical in vitro pharmacology approaches to establish that AB‐MDMSBA does not bind to or activate the cannabinoid CB1 receptor and therefore should not be classified as a synthetic cannabinoid receptor agonist. ABSTRACT Synthetic cannabinoids remain one of the most prominent classes of New Psychoactive Substances (NPS).
Patel M, Ganeshan D, Horgan I, Partington H, Glass M.   +4 more
europepmc   +2 more sources

CCL19 and CCL21 Display Reciprocal Dependence on R209 in ECL2 of CCR7 for Receptor Activation, Relating to Differences in the Chemokine N-Terminal Region. [PDF]

Basic Clin Pharmacol Toxicol
ABSTRACT CCL19 and CCL21 interact differentially with their shared receptor CCR7. A key amino acid important for CCL19‐mediated receptor activation is arginine 209 (R209) in extracellular loop 2 (ECL2) of CCR7. CCL21 activation of CCR7, in contrast, does not depend on R209.
Rosenkilde MM, Volkman BF, Peterson FC, Veldkamp CT, Hjortø GM.   +4 more
europepmc   +2 more sources

Insights into the bioluminescence systems of three sea pens (Cnidaria: Anthozoa): from de novo transcriptome analyses to biochemical assays [PDF]

Open Biology
Bioluminescence is the production of visible light by living organisms. It occurs through the oxidation of luciferin substrates catalysed by luciferase enzymes.
Laurent Duchatelet, Gabriela A. Galeazzo, Constance Coubris, Laure Bridoux, René Rezsohazy, Marcelo R. S. Melo, Martin Marek, Danilo T. Amaral, Sam Dupont, Anderson Garbuglio de Oliveira, Jérôme Delroisse   +10 more
doaj   +2 more sources

Design of Ctenophore Ca²⁺-Regulated Photoprotein Berovin Capable of Being Converted into Active Protein Under Physiological Conditions: Computational and Experimental Approaches [PDF]

Life
Here, we describe (1) the AlphaFold-based structural modeling approach to identify amino acids of the photoprotein berovin that are crucial for coelenterazine binding, and (2) the production and characterization of berovin mutants with substitutions of ...
Ludmila P. Burakova, Nikita V. Ivanisenko, Natalia V. Rukosueva, Vladimir A. Ivanisenko, Eugene S. Vysotski   +4 more
doaj   +2 more sources

Limitations of the p16-3MR mouse model for detecting and eliminating senescent cells [PDF]

EMBO Reports
The p16-3MR mouse model, designed to express Renilla luciferase, mRFP, and herpes simplex virus 1 thymidine kinase (HSV-TK) under the p16 INK4a promoter, has been widely used to visualize and ablate senescent cells in vivo, but our analyses revealed ...
Nozomi Hori, Shimpei Kawamoto, Ken Uemura, Yumi Kinugasa-Katayama, Yumiko Okumura, Kentaro Tanaka, Jeong Hoon Park, Masahiro Wakita, Daisuke Motooka, Naoko Ohtani, Eiji Hara   +10 more
doaj   +2 more sources

Advanced Bioluminescence Reporter with Engineered Gaussia Luciferase via Sequence-Guided Mutagenesis [PDF]

Biosensors
Gaussia luciferase (GLuc) is the preeminent secreted luciferase widely used in cell-based reporter assays. By employing sequence-guided mutagenesis informed by alignments of diverse copepod luciferase sequences, we identified key amino acids that ...
Vinayakumar Gedi, Eun Hye Kim, Bohyun Oh, Young-Pil Kim   +3 more
doaj   +2 more sources