KCS1 and VIP1, the genes encoding yeast phosphoinositol pyrophosphate synthases, are required for Ca2+‐mediated response to dimethylsulfoxide (DMSO) [PDF]
FEBS Open BioDimethylsulfoxide (DMSO) is widely used as a solvent or as a carrier when screening for biologic activity of various chemicals, but results need to be interpreted carefully due to its intrinsic toxicity.
Larisa Ioana Gogianu +4 more
doaj +3 more sources
Shedding Light on Calcium Dynamics in the Budding Yeast: A Review on Calcium Monitoring with Recombinant Aequorin [PDF]
MoleculesRecombinant aequorin has been extensively used in mammalian and plant systems as a powerful tool for calcium monitoring. While aequorin has also been widely applied in yeast research, a notable gap exists in the literature regarding comprehensive reviews
Larisa Ioana Gogianu +2 more
doaj +2 more sources
Red-Shifted Aequorin Variants Incorporating Non-Canonical Amino Acids: Applications in In Vivo Imaging [PDF]
PLoS ONE, 2016 The increased importance of in vivo diagnostics has posed new demands for imaging technologies. In that regard, there is a need for imaging molecules capable of expanding the applications of current state-of-the-art imaging in vivo diagnostics.
Laura Rowe +2 more
exaly +2 more sources
PLoS ONE, 2011 Bioluminescence recording of Ca(2+) signals with the photoprotein aequorin does not require radiative energy input and can be measured with a low background and good temporal resolution.
Juan Llopis
exaly +3 more sources
Fast kinetics of photoprotein emitting species [PDF]
Scientific ReportsCoelenterazine is the most common substrate for light-emitting reactions identified in luminous marine organisms. Among bioluminescent proteins engaging coelenterazine as a luciferin, Ca2+-regulated photoproteins form stable enzyme-substrate complexes ...
Elena V. Eremeeva +4 more
doaj +2 more sources
Acidic Stress Induces Cytosolic Free Calcium Oscillation, and an Appropriate Low pH Helps Maintain the Circadian Clock in Arabidopsis [PDF]
PlantsAcidic stress is a formidable environmental factor that exerts adverse effects on plant growth and development, ultimately leading to a potential reduction in agricultural productivity.
Wei Chen +5 more
doaj +2 more sources
Systematic functional evaluation of CNGA1 missense variants associated with retinitis pigmentosa [PDF]
Molecular MedicineBackground Missense variants are frequently classified as variants of uncertain significance (VUS) according to the guidelines of the American College of Medical Genetics and Genomics and the Association of Molecular Pathology (ACMG/AMP).
Peggy Reuter +10 more
doaj +2 more sources
Suppression of Contraction Raises Calcium Ion Levels in the Heart of Zebrafish Larvae [PDF]
BiosensorsZebrafish larvae have emerged as a valuable model for studying heart physiology and pathophysiology, as well as for drug discovery, in part thanks to its transparency, which simplifies microscopy.
Antonio Martinez-Sielva +5 more
doaj +2 more sources
Regulation of myocardial contraction as revealed by intracellular Ca2+ measurements using aequorin [PDF]
Journal of Physiological SciencesOf the ions involved in myocardial function, Ca2+ is the most important. Ca2+ is crucial to the process that allows myocardium to repeatedly contract and relax in a well-organized fashion; it is the process called excitation–contraction coupling.
Satoshi Kurihara, Norio Fukuda
doaj +2 more sources
Analysis of abiotic and biotic stress-induced Ca2+ transients in the crop species Solanum tuberosum [PDF]
Scientific ReportsSecondary messengers, such as calcium ions (Ca2+), are integral parts of a system that transduces environmental stimuli into appropriate cellular responses.
Annelotte van Dieren +4 more
doaj +2 more sources