Results 31 to 40 of about 3,245 (169)
Measuring [Ca2+] in the endoplasmic reticulum with aequorin [PDF]
Cell Calcium, 2002 The photoprotein aequorin was the first probe used to measure specifically the [Ca(2+)] inside the lumen of the endoplasmic reticulum ([Ca(2+)](ER)) of intact cells and it provides values for the steady-state [Ca(2+)](ER), around 500 microM, that closely match those obtained now by other procedures.
Álvarez Martín, Javier +1 more
openaire +3 more sources
Frontiers in Plant Science, 2014 Calcium (Ca2+) is a second messenger involved in many plant signalling processes. Biotic and abiotic stimuli induce Ca2+ signals within plant cells, which, when decoded, enable these cells to adapt in response to environmental stresses. Multiple examples
Tou Cheu eXiong +5 more
doaj +1 more source
STAR Protocols, 2021 Summary: Environmental stimuli evoke transient increases of the cytosolic Ca2+ level. To identify upstream components of Ca2+ signaling, we have optimized two forward genetic screening systems based on Ca2+ reporter aequorin.
Shujing Sun +4 more
doaj +1 more source
Bio-Protocol, 2021 Calcium signaling is an emerging mechanism by which bacteria respond to environmental cues. To measure the intracellular free-calcium concentration in bacterial cells, [Ca2+]i, a simple spectrofluorometric method based on the chemical probe Fura 2 ...
Simone Trabalza +5 more
doaj +1 more source
Smart Molecules, EarlyView.Nonthermal ultrasound transforms acoustic energy into diverse therapeutic bioeffects through cavitation, mechanical stimulation, and physicochemical activation. This review discusses how acoustic parameters, biological mechanisms, and responsive materials interact to influence nonthermal ultrasound‐enabled cancer therapies. Mechanism‐guided engineering
Liu Wang +5 more
wiley +1 more source
The crystal structures of semi‐synthetic aequorins [PDF]
Protein Science, 2005 AbstractThe photoprotein aequorin emits light by an intramolecular reaction in the presence of a trace amount of Ca2+. Semi‐synthetic aequorins, produced by replacing the coelenterazine moiety in aequorin with the analogues of coelenterazine, show widely different sensitivities to Ca2+.
Sachiko, Toma +5 more
openaire +2 more sources
New Phytologist, EarlyView.Model of the cell wall damage (CWD)‐responsive signaling network leading to camalexin biosynthesis. Summary Plant cell walls constitute dynamic barriers that are essential for defense against pathogens. Beyond a structural role in blocking pathogen infection, innate mechanisms monitor cell wall integrity (CWI) in Arabidopsis thaliana and trigger local ...
Richard Noi Morton +9 more
wiley +1 more source
Sensors, 2009 Whole-cell, genetically modified bioreporters are designed to emit detectable signals in response to a target analyte or related group of analytes. When integrated with a transducer capable of measuring those signals, a biosensor results that acts as a ...
Gary S. Sayler +2 more
doaj +1 more source
Aequorin variants with improved bioluminescence properties [PDF]
Protein Engineering Design and Selection, 2009 The photoprotein aequorin has been widely used as a bioluminescent label in immunoassays, for the determination of calcium concentrations in vivo, and as a reporter in cellular imaging. It is composed of apoaequorin (189 amino acid residues), the imidazopyrazine chromophore coelenterazine and molecular oxygen.
E, Dikici +7 more
openaire +2 more sources
A tomato MIK2‐clade receptor is involved in the perception of a Fusarium‐derived elicitor
New Phytologist, Volume 251, Issue 5, Page 2777-2798, September 2026.Fusarium elicitor responsivness and MIK2‐clade proteins are conserved across plant families. Summary Plants continuously monitor their environment to adapt to threats affecting health and fitness. Cell‐surface receptors detect conserved microbe‐associated molecular patterns (MAMPs) or endogenous immunogenic signals to activate signaling pathways that ...
Julian Maroschek +6 more
wiley +1 more source