Results 31 to 40 of about 2,993 (171)
Correction: Regulation of myocardial contraction as revealed by intracellular Ca2+ measurements using aequorin [PDF]
Journal of Physiological SciencesSatoshi Kurihara, Norio Fukuda
doaj +2 more sources
Aequorin variants with improved bioluminescence properties [PDF]
Protein Engineering Design and Selection, 2009 The photoprotein aequorin has been widely used as a bioluminescent label in immunoassays, for the determination of calcium concentrations in vivo, and as a reporter in cellular imaging. It is composed of apoaequorin (189 amino acid residues), the imidazopyrazine chromophore coelenterazine and molecular oxygen.
E, Dikici +7 more
openaire +2 more sources
STAR Protocols, 2021 Summary: Environmental stimuli evoke transient increases of the cytosolic Ca2+ level. To identify upstream components of Ca2+ signaling, we have optimized two forward genetic screening systems based on Ca2+ reporter aequorin.
Shujing Sun +4 more
doaj +1 more source
The crystal structures of semi‐synthetic aequorins [PDF]
Protein Science, 2005 AbstractThe photoprotein aequorin emits light by an intramolecular reaction in the presence of a trace amount of Ca2+. Semi‐synthetic aequorins, produced by replacing the coelenterazine moiety in aequorin with the analogues of coelenterazine, show widely different sensitivities to Ca2+.
Sachiko, Toma +5 more
openaire +2 more sources
Bio-Protocol, 2021 Calcium signaling is an emerging mechanism by which bacteria respond to environmental cues. To measure the intracellular free-calcium concentration in bacterial cells, [Ca2+]i, a simple spectrofluorometric method based on the chemical probe Fura 2 ...
Simone Trabalza +5 more
doaj +1 more source
Cancer Medicine, Volume 15, Issue 4, April 2026.We developed a novel, noninvasive diagnostic method for colorectal cancer using sensor cells expressing insect ORs that detect volatile organic compounds in urine samples. By analyzing the luminescence responses of sensor cells and applying machine learning, our model achieved 80% sensitivity and an ROC‐AUC of 0.84 in distinguishing colorectal cancer ...
Koji Ando +11 more
wiley +1 more source
The power of ionic movements in plants
New Phytologist, Volume 249, Issue 5, Page 2232-2240, March 2026.Summary The movement of ion‐driven electrogenic events known as plant action potentials in the Venus flytrap Dionaea muscipula has first been recognized in Darwin's time. Besides electrophysiological techniques making use of current‐ and voltage‐recording electrodes, today an ever‐growing spectrum of tools has become available, that report online ...
Rainer Hedrich, Ines Kreuzer
wiley +1 more source
Sensors, 2009 Whole-cell, genetically modified bioreporters are designed to emit detectable signals in response to a target analyte or related group of analytes. When integrated with a transducer capable of measuring those signals, a biosensor results that acts as a ...
Gary S. Sayler +2 more
doaj +1 more source
Imaging malaria parasites across scales and time
Journal of Microscopy, Volume 301, Issue 2, Page 122-150, February 2026.Abstract The idea that disease is caused at the cellular level is so fundamental to us that we might forget the critical role microscopy played in generating and developing this insight. Visually identifying diseased or infected cells lays the foundation for any effort to curb human pathology.
Julien Guizetti
wiley +1 more source
New Phytologist, Volume 249, Issue 4, Page 1644-1658, February 2026.Summary Calcium (Ca2+) signaling is integral to nearly all aspects of plant biology, including development and responses to biotic and abiotic stresses. It operates through two main layers: the generation of Ca2+ signals and their decoding by Ca2+‐binding proteins, which act early in diverse signaling pathways. The system exhibits remarkable robustness
Chunxia Zhang, Yang Song, Jörg Kudla
wiley +1 more source