Results 21 to 30 of about 3,245 (169)
Aequorin as a Useful Calcium-Sensing Reporter in Candida albicans
Journal of Fungi, 2021 In Candida albicans, calcium ions (Ca2+) regulate the activity of several signaling pathways, especially the calcineurin signaling pathway. Ca2+ homeostasis is also important for cell polarization, hyphal extension, and plays a role in contact sensing ...
Dominique Sanglard
doaj +1 more source
Plants, 2021 Increasing evidence indicates that water activated by plasma discharge, termed as plasma-activated water (PAW), can promote plant growth and enhance plant defence responses. Nevertheless, the signalling pathways activated in plants in response to PAW are
Enrico Cortese +8 more
doaj +1 more source
CANARY Technology: Working Principles of Biosensor, Detection of Infectious Pathogens
Вестник войск РХБ защиты, 2020 Reusable biosensors for the detection of biochemical analytes are widely used in clinical and laboratory practice. However, biosensors for the detection of pathogenic microorganisms are still under development or implementation.
A. S. Gorshkov +2 more
doaj +1 more source
Bioluminescence Monitoring of Neuronal Activity in Freely Moving Zebrafish Larvae
Bio-Protocol, 2017 The proof of concept for bioluminescence monitoring of neural activity in zebrafish with the genetically encoded calcium indicator GFP-aequorin has been previously described (Naumann et al., 2010) but challenges remain.
Steven Knafo +4 more
doaj +1 more source
Ca2+ Measurements in Mammalian Cells with Aequorin-based Probes
Bio-Protocol, 2017 Aequorin is a Ca2+ sensitive photoprotein suitable to measure intracellular Ca2+ transients in mammalian cells. Thanks to recombinant cDNAs expression, aequorin can be specifically targeted to various subcellular compartments, thus allowing an accurate ...
Anna Tosatto +2 more
doaj +1 more source
Recombinant aequorin and recombinant semi-synthetic aequorins. Cellular Ca2+ ion indicators [PDF]
Biochemical Journal, 1990 Properties of a recombinant aequorin were investigated in comparison with those of natural aequorin. In chromatographic behaviour the recombinant aequorin did not match any of ten isoaequorins tested, although it was very similar to aequorin J. Its sensitivity to Ca2+ was found to be higher than that of any isoaequorin except aequorin D.
O, Shimomura +3 more
openaire +2 more sources
Red-Shifted Aequorin-Based Bioluminescent Reporters for in Vivo Imaging of Ca Signaling
Molecular Imaging, 2007 Real-time visualization of calcium (Ca 2+ ) dynamics in the whole animal will enable important advances in understanding the complexities of cellular function.
Thomas Curie +3 more
doaj +1 more source
Measurement of Intracellular Calcium Concentration in Pseudomonas aeruginosa
Bio-Protocol, 2016 Characterization of the molecular mechanisms of calcium (Ca2+) regulation of bacterial physiology and virulence requires tools enabling measuring and monitoring the intracellular levels of free calcium (Ca2+in).
Manita Guragain +2 more
doaj +1 more source
Frontiers in Plant Science, 2018 Salinity is one of the formidable environmental factors that affect plant growth and development and constrain agricultural productivity. Experimentally imposed short-term NaCl treatment triggers a transient increase in cytosolic free Ca2+ concentration (
Lulu Liu +11 more
doaj +1 more source
Plant Immune Memory in Systemic Tissue Does Not Involve Changes in Rapid Calcium Signaling
Frontiers in Plant Science, 2021 Upon pathogen recognition, a transient rise in cytoplasmic calcium levels is one of the earliest events in plants and a prerequisite for defense initiation and signal propagation from a local site to systemic plant tissues.
Bernadette Eichstädt +11 more
doaj +1 more source