Results 21 to 30 of about 2,993 (171)
CANARY Technology: Working Principles of Biosensor, Detection of Infectious Pathogens
Вестник войск РХБ защиты, 2020 Reusable biosensors for the detection of biochemical analytes are widely used in clinical and laboratory practice. However, biosensors for the detection of pathogenic microorganisms are still under development or implementation.
A. S. Gorshkov +2 more
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Recombinant aequorin and recombinant semi-synthetic aequorins. Cellular Ca2+ ion indicators [PDF]
Biochemical Journal, 1990 Properties of a recombinant aequorin were investigated in comparison with those of natural aequorin. In chromatographic behaviour the recombinant aequorin did not match any of ten isoaequorins tested, although it was very similar to aequorin J. Its sensitivity to Ca2+ was found to be higher than that of any isoaequorin except aequorin D.
O, Shimomura +3 more
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Bioluminescence Monitoring of Neuronal Activity in Freely Moving Zebrafish Larvae
Bio-Protocol, 2017 The proof of concept for bioluminescence monitoring of neural activity in zebrafish with the genetically encoded calcium indicator GFP-aequorin has been previously described (Naumann et al., 2010) but challenges remain.
Steven Knafo +4 more
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Ca2+ Measurements in Mammalian Cells with Aequorin-based Probes
Bio-Protocol, 2017 Aequorin is a Ca2+ sensitive photoprotein suitable to measure intracellular Ca2+ transients in mammalian cells. Thanks to recombinant cDNAs expression, aequorin can be specifically targeted to various subcellular compartments, thus allowing an accurate ...
Anna Tosatto +2 more
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Red-Shifted Aequorin-Based Bioluminescent Reporters for in Vivo Imaging of Ca Signaling
Molecular Imaging, 2007 Real-time visualization of calcium (Ca 2+ ) dynamics in the whole animal will enable important advances in understanding the complexities of cellular function.
Thomas Curie +3 more
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Measurement of Intracellular Calcium Concentration in Pseudomonas aeruginosa
Bio-Protocol, 2016 Characterization of the molecular mechanisms of calcium (Ca2+) regulation of bacterial physiology and virulence requires tools enabling measuring and monitoring the intracellular levels of free calcium (Ca2+in).
Manita Guragain +2 more
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Measuring [Ca2+] in the endoplasmic reticulum with aequorin [PDF]
Cell Calcium, 2002 The photoprotein aequorin was the first probe used to measure specifically the [Ca(2+)] inside the lumen of the endoplasmic reticulum ([Ca(2+)](ER)) of intact cells and it provides values for the steady-state [Ca(2+)](ER), around 500 microM, that closely match those obtained now by other procedures.
Álvarez Martín, Javier +1 more
openaire +3 more sources
Frontiers in Plant Science, 2018 Salinity is one of the formidable environmental factors that affect plant growth and development and constrain agricultural productivity. Experimentally imposed short-term NaCl treatment triggers a transient increase in cytosolic free Ca2+ concentration (
Lulu Liu +11 more
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Plant Immune Memory in Systemic Tissue Does Not Involve Changes in Rapid Calcium Signaling
Frontiers in Plant Science, 2021 Upon pathogen recognition, a transient rise in cytoplasmic calcium levels is one of the earliest events in plants and a prerequisite for defense initiation and signal propagation from a local site to systemic plant tissues.
Bernadette Eichstädt +11 more
doaj +1 more source
Frontiers in Plant Science, 2014 Calcium (Ca2+) is a second messenger involved in many plant signalling processes. Biotic and abiotic stimuli induce Ca2+ signals within plant cells, which, when decoded, enable these cells to adapt in response to environmental stresses. Multiple examples
Tou Cheu eXiong +5 more
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