Results 11 to 20 of about 3,245 (169)

Analysis of abiotic and biotic stress-induced Ca2+ transients in the crop species Solanum tuberosum [PDF]

Scientific Reports
Secondary messengers, such as calcium ions (Ca2+), are integral parts of a system that transduces environmental stimuli into appropriate cellular responses.
Annelotte van Dieren, Roland E. Schwarzenbacher, Sophia Sonnewald, Andras Bittner, Ute C. Vothknecht   +4 more
doaj   +2 more sources

Synthesis and Biological Activity of 2,6-Disubstituted and 2,6,9-Trisubstituted 7-Deazapurine Nucleobases. [PDF]

ChemMedChem
Four series of 20 (het)aryl substituted 7‐deazapurine nucleobases were synthesized. Several potent antagonists of all four subtypes of adenosine receptors were identified by functional assay. High‐throughput screening of the IMTM compound library for modulatory activity on adenosine receptors (ARs) identified two deazapurine hits based on 2,6‐diaryl‐7 ...
Šinkevičiūtė U, Burgetová Ječmeňová K, Gurská S, Poštová Slavětínská L, Baran V, Polishchuk P, Džubák P, Hocek M, Hajdúch M, Tichý M.   +9 more
europepmc   +2 more sources

Jasmonate primes plant responses to extracellular ATP. [PDF]

Plant J
SUMMARY Extracellular ATP (eATP) signaling in Arabidopsis thaliana is mediated by the purinoceptor P2K1. Previous studies have clarified that the downstream transcriptional responses to eATP involve jasmonate (JA)‐based signaling components such as the JA receptor (COI1) and JA‐responsive bHLH transcription factors (MYCs).
Jewell JB, Carlton AS, Tolley JP, Bartley LE, Tanaka K.   +4 more
europepmc   +2 more sources

Resveratrol Affects Cell Activities, Induces Apoptosis and Regulates AMPK Signaling Pathway in Pleural Mesothelioma Cells. [PDF]

FASEB J
This graphical abstract presents the study of Resveratrol as a potential adjuvant therapy for Pleural Mesothelioma (PM), focusing on the epithelioid and biphasic histotypes using IST‐MES 2 and MSTO‐211H cell lines. Following 24 h of cell seeding, the cells were treated with Resveratrol, and various analyses were conducted to assess its effects.
Iaquinta MR, De Pace R, Benkhalqui A, Pesaresi C, Patergnani S, Righes G, Pinton P, Tognon M, Martini F, Mazzoni E.   +9 more
europepmc   +2 more sources

High-Vigor Maize Seeds Resist Fusarium graminearum Infection through Stronger Ca²⁺ Signaling

Agriculture, 2022
Seeds with high vigor have strong resistance to various adverse environmental conditions. However, little is known about how seed vigor affects the resistance of seeds to biotic stress.
Baokuan Xu, Xiyan Liu, Xuejiao Song, Qifang Guo, Yongqi Yin, Chunqing Zhang, Yan Li   +6 more
doaj   +1 more source

Cardioluminescence in Transgenic Zebrafish Larvae: A Calcium Imaging Tool to Study Drug Effects and Pathological Modeling

Biomedicines, 2021
Zebrafish embryos and larvae have emerged as an excellent model in cardiovascular research and are amenable to live imaging with genetically encoded biosensors to study cardiac cell behaviours, including calcium dynamics. To monitor calcium ion levels in
Manuel Vicente, Jussep Salgado-Almario, Michelle M. Collins, Antonio Martínez-Sielva, Masafumi Minoshima, Kazuya Kikuchi, Beatriz Domingo, Juan Llopis   +7 more
doaj   +1 more source

Development of visible light-sensitive human neuropsin (OPN5) via single amino acid substitution. [PDF]

FEBS Lett
The present study determines a key amino acid residue, Lys91, for defining UV sensitivity of human OPN5. Heterologous action spectroscopy of the wild type and K91 mutants of OPN5 in HEK293T cells reveals that substitution of Lys91 with neutral (alanine) or acidic amino acids (glutamic or aspartic acids) causes substantial shifts in spectral sensitivity
Sakai Y, McDowell RJ, Lucas RJ.
europepmc   +2 more sources

Aequorin mutants with increased thermostability [PDF]

Analytical and Bioanalytical Chemistry, 2014
Bioluminescent labels can be especially useful for in vivo and live animal studies due to the negligible bioluminescence background in cells and most animals, and the non-toxicity of bioluminescent reporter systems. Significant thermal stability of bioluminescent labels is essential, however, due to the longitudinal nature and physiological temperature
Xiaoge, Qu, Laura, Rowe, Emre, Dikici, Mark, Ensor, Sylvia, Daunert   +4 more
openaire   +2 more sources

Chiral deaza-coelenterazine analogs for probing a substrate-binding site in the Ca2+-binding photoprotein aequorin.

PLoS ONE, 2021
The Ca2+-binding photoprotein aequorin is a complex of apoAequorin (apoprotein) and (S)-2-peroxycoelenterazine. Aequorin can be regenerated by the incubation of apoAequorin with coelenterazine and molecular oxygen (O2).
Satoshi Inouye, Yuto Sumida, Yuri Tomabechi, Jumpei Taguchi, Mikako Shirouzu, Takamitsu Hosoya   +5 more
doaj   +1 more source

Imaging of Endoplasmic Reticulum Ca2+ in the Intact Pituitary Gland of Transgenic Mice Expressing a Low Affinity Ca2+ Indicator

Frontiers in Endocrinology, 2021
The adenohypophysis contains five secretory cell types (somatotrophs, lactotrophs, thyrotrophs, corticotrophs, and gonadotrophs), each secreting a different hormone, and controlled by different hypothalamic releasing hormones (HRHs).
Jonathan Rojo-Ruiz, Paloma Navas-Navarro, Lucía Nuñez, Javier García-Sancho, María Teresa Alonso   +4 more
doaj   +1 more source